FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003127416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003127416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2827315
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC898893.179306161499514TruSeq Adapter, Index 3 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC247740.8762377025552511TruSeq Adapter, Index 3 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAACTCGTATGCC116410.41173339369684664TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC50060.1770584459106962TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAACTCGTATGC31900.11282789501700376TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCACCTCGTATGC31890.11279252577091692TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)