FastQCFastQC Report
Mon 3 Apr 2023
EGAF00003211195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003211195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69124788
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGG1855190.2683827399224718No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCT1429690.20682739743086084No Hit
CTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGC1371480.19840639511256078No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1277100.18475282701771178No Hit
CTCTGCTCTTCTCCAGAAGGCACCACCACAGCTGCCCACTTCTGGAAGGT1134830.1641712087420796No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT1025330.14833029216668267No Hit
CTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTG1005000.14538923432213638No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA1002940.1450912225582522No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC853200.12342894997377787No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA838100.12124449481132585No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG784610.11350631556367305No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGT750550.10857899484624821No Hit
GCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACT738010.10676488440007946No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG719800.10413051827370523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)