FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153525
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA43122.808663084188243No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG42412.7624165445367206No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC38232.490148184334799No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG27391.7840742550073279No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT25041.6310047223579223No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG25001.6283992835043153No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC22541.4681647940074907No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG22361.4564403191662596No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC21841.4225696140693698No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC21781.4186614557889594No Hit
TGTAAAACGACGGCCAGTTGAGAGGGACTGGAGAAAGGGAACTTACAGAG19901.296205829669435No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC19701.2831786354014003No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC19021.2388861748900832No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA18881.229767138902459No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC18121.1802638006839277No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT17941.1685393258426966No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC17661.1503012538674484No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG15451.006350757205667No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA15210.9907181240840254No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA15010.9776909298159908No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG14640.9535906204201271No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA14100.9184171958964338No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC13820.9001791239211856No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG12700.8272268360201921No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG12210.7953102100635075No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC12130.7900993323562937No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC12060.7855398143624817No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC11880.7738153395212506No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC11480.7477609509851816No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT11320.7373391955707539No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC11170.727568799869728No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC11040.7191011235955056No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG10890.7093307278944797No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC10650.6936980947728383No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT10160.6617814688161537No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA9560.6226998860120502No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC9460.6161862888780328No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC9430.6142322097378277No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCCT9140.5953427780491777No Hit
TGTAAAACGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC8910.5803615046409379No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG8840.5758019866471259No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG8570.5582152743852793No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA8490.5530043966780654No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA8470.5517016772512621No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT8350.5438853606904412No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA8000.5210877707213809No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT8000.5210877707213809No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG7960.5184823318677739No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7880.5132714541605602No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA7850.511317375020355No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA7840.5106660153069532No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT7830.5100146555935515No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA7750.5048037778863378No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT7700.5015469793193291No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC7390.4813548282038756No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG7120.463768115942029No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG6880.4481354828203875No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC6610.43054877055854096No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG6570.4279433317049341No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6380.4155674971503012No Hit
TGTAAAACGACGGCCAGTCCTCACTCCCCACCCAGGATCTGGGCACTGGA6360.4142647777234978No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT6360.4142647777234978No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT6260.40775118058948057No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG6210.40449438202247195No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6180.4025403028822668No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT6140.39993486402865985No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG6090.3966780654616512No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT5790.3771372740595994No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG5750.37453183520599254No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA5730.37322911577918905No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA5670.3693209574987787No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT5630.36671551864517177No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA5580.36345872007816316No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC5540.3608532812245563No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC5520.3595505617977528No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5500.35824784237094937No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5480.3569451229441459No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG5310.3458720078163166No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA5220.340009770395701No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG5060.3295880149812734No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT5000.3256798567008631No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT4710.306790425012213No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4660.3035336264452044No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG4440.2892037127503664No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT4370.2846441947565543No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4270.27813059762253706No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG4100.2670574824947077No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT4020.2618466047874939No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGCCCGGATGT3950.2572870867936818No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG3880.25272756879986974No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG3870.252076209086468No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC3840.2501221299462628No Hit
TGTAAAACGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC3800.2475166910926559No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG3740.24360853281224557No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA3480.2266731802638007No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3460.22537046083699724No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGGACCCAGAG3270.21299462628236446No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT3210.20908646800195407No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT3080.20061879172773167No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTGGGCTCACT2950.1921511154535092No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT2860.18628887803289368No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT2840.18498615860609022No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA2840.18498615860609022No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCACGGCCAGGTGT2810.18303207946588504No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT2810.18303207946588504No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT2770.18042664061227814No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC2720.1771698420452695No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2720.1771698420452695No Hit
TGTAAAACGACGGCCAGTCAACCTCAGGCGATCCACCCGCCTCGGCCTCC2650.1726103240514574No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2530.1647940074906367No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGCACTCACGCAA2510.16349128806383326No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG2500.16283992835043154No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2490.1621885686370298No Hit
TGTAAAACGACGGCCAGTGGAACTGGTGGGCAGTGTCCCAAGAGGTGGGG2370.15437225207620908No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTTACT2280.14851001465559358No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2260.14720729522879009No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC2240.14590457580198665No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2220.1446018563751832No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG2180.14199641752157627No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCGTCTACACGAGA2110.13743689952776422No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG2090.13613418010096073No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC2090.13613418010096073No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2050.13352874124735384No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG2030.1322260218205504No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC2010.13092330239374694No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2000.13027194268034523No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG1950.1270151441133366No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1950.1270151441133366No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCCAAAGTGCTGGG1900.12375834554632795No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA1750.11398794984530207No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGTAAAACGACGG1750.11398794984530207No Hit
TGTAAAACGACGGCCAGTACCTGAATCTTACTAAGCGCCTGGATCTATTG1700.11073115127829343No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA1690.11007979156489171No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT1610.1048689138576779No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT1590.10356619443087445No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1580.10291483471747273No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1560.10161211529066928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)