FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138504
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT33972.4526367469531567No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT32862.372494657194016No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT30732.218708484953503No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT20181.4569976318373477No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG19801.429561601108993No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT19471.4057355744238433No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT18881.36313752671403No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC18641.345809507306648No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT15941.1508692889736036No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA14171.0230751458441634No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT13450.9710910876220181No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC13290.9595390746837635No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT13110.9465430601282273No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT12960.9357130479986138No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT12630.911887021313464No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT12510.903223011609773No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT12240.8837289897764685No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT11230.810806908103737No Hit
CAGGAAANNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT9080.6555767342459423No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT8920.6440247213076878No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA8280.5978166695546699No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA8190.5913186622769018No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG8080.5833766533818517No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT8030.5797666493386472No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC7530.543666608906602No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT7500.5415006064806793No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7460.5386126032461157No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA7370.5321145959683475No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT7310.527782591116502No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA7040.5082885692831977No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA6380.46063651591289784No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA5290.3819384277710391No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA5230.37760642291919366No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA5160.3725524172587073No Hit
CAGGAAANNGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC4900.3537803962340438No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT4850.3501703921908393No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT4750.3429503841044302No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4440.3205683590365621No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT4420.3191243574192803No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT4180.30179633801189854No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT3690.2664182983884942No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3640.2628082943452897No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3580.25847628949344426No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3540.25558828625888064No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3510.2534222838329579No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3380.2440362733206261No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3310.2389822676601398No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA3300.23826026685149887No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3230.2332062611910125No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3200.2310402587650898No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3170.22887425633916708No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3130.2259862531046035No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3080.22237624906139894No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA3060.22093224744411713No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3040.21948824582683535No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3040.21948824582683535No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC3010.2173222434009126No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2940.21226823774042627No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2920.21082423612314447No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2910.21010223531450356No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2900.20938023450586263No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2880.20793623288858082No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2870.20721423207993991No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2860.20649223127129904No Hit
CAGGAAANNGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT2840.2050482296540172No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2770.1999942239935309No Hit
CAGGAAANAGCTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2730.19710622075896725No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2730.19710622075896725No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2700.19494021833304453No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGGAGATATATGTC2630.1898862126725582No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC2590.18699820943799458No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2580.18627620862935365No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2570.18555420782071275No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2560.18483220701207187No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2540.18338820539479003No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2440.17616819730838099No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2390.17255819326517646No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2310.16678218679604923No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2280.1646161843701265No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2240.16172818113556287No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2230.16100618032692196No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2170.15667417547507653No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2160.15595217466643563No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2020.14584416334546293No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2000.14440016172818113No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2000.14440016172818113No Hit
NAGGAAANNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1950.1407901576849766No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1910.13790215445041298No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1910.13790215445041298No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC1900.13718015364177208No Hit
NAGGANANNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1890.13645815283313117No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1890.13645815283313117No Hit
CAGGANANNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1850.13357014959856756No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1850.13357014959856756No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1840.13284814878992665No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1800.12996014555536303No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1790.12923814474672213No Hit
CAGGAAACAGCTATGANCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1720.12418413908623577No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1710.12346213827759488No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1700.12274013746895397No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1660.11985213423439034No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1650.11913013342574943No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1620.11696413099982672No Hit
NAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1620.11696413099982672No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1560.11263212614798128No Hit
CAGGAAACNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1540.11118812453069947No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1540.11118812453069947No Hit
CAGGAAACAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1530.11046612372205857No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1490.10757812048749495No Hit
NAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1490.10757812048749495No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1460.10541211806157223No Hit
CAGGAANNNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1410.1018021140183677No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1400.1010801132097268No Hit
NAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1390.10035811240108589No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1390.10035811240108589No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC1390.10035811240108589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)