FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003245512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36628
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT11083.0250081904553894No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT8952.4434858578136947No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8262.2551053838593424No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT7181.9602489898438353No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT6191.689963961996287No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4931.345964835644862No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3941.0756798077973135No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3831.0456481380364748No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3560.971934039532598No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT3200.8736485748607623No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT3090.8436169050999236No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3030.8272359943212843No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2980.8135852353390849No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2670.7289505296494485No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2440.6661570383313312No Hit
CAGGAAANAGCTATGANNTGCAATCTTGAGCTCCTGGGCCAGGAAACAGG2430.6634268865348913No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2120.5787921808452551No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1820.4968876269520585No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1750.47777656437697935No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1640.44774489461614064No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1580.43136398383750135No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1570.4286338320410614No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1540.42044337665174186No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1500.4095227694659824No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1490.40679261766954244No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1360.371300644315824No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1260.34399912635142516No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1210.33034836736922574No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1170.3194277601834662No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1160.3166976083870263No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1160.3166976083870263No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1130.30850715299770665No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1130.30850715299770665No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1130.30850715299770665No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1110.3030468494048269No Hit
CAGGAAANNGCTATGANNTGCAATCTTGAGCTCCTGGGCCAGGAAACAGG1090.29758654581194716No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1060.28939609042262754No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1050.2866659386261876No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1030.2812056350333078No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1030.2812056350333078No Hit
CAGGAAACAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT1010.27574533144042807No Hit
CAGGAAACAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT1000.2730151796439882No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT980.26755487605110845No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT940.2566342688653489No Hit
CAGGAAACNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT870.23752320629026974No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC860.23479305449382987No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT830.2266025991045102No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA800.21841214371519058No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT800.21841214371519058No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG790.21568199191875068No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC740.20203123293655126No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC730.19930108114011139No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT670.1829201703614721No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC670.1829201703614721No Hit
CAGGAAACNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT670.1829201703614721No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC670.1829201703614721No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA630.17199956317571258No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA620.16926941137927268No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG600.16380910778639293No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA590.16107895598995303No Hit
CAGGAAANNGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC580.15834880419351316No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT570.15561865239707326No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT550.15015834880419351No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT510.13923774161843397No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT490.13377743802555422No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC490.13377743802555422No Hit
CAGGAAACNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG490.13377743802555422No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT490.13377743802555422No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT480.13104728622911435No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT480.13104728622911435No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT480.13104728622911435No Hit
CAGGAAANNGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC470.12831713443267445No Hit
CAGGAANNNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT460.12558698263623458No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT450.12285683083979469No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT440.12012667904335482No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT440.12012667904335482No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT430.11739652724691493No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT420.11466637545047503No Hit
CAGGAAACAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG420.11466637545047503No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT410.11193622365403516No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT410.11193622365403516No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT410.11193622365403516No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT400.10920607185759529No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC390.1064759200611554No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT380.10374576826471553No Hit
CAGGAAANAGCTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG370.10101561646827563No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG370.10101561646827563No Hit
CAGGAAACAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT370.10101561646827563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)