FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132028
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA31192.3623776774623564No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA29702.2495228284909263No Hit
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA24661.86778562123186No Hit
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG23351.7685642439482536No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG22501.7041839609779743No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA21951.662526130820735No Hit
TGTAAAANGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG21371.6185960553821919No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG19791.4989244705668494No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT16841.2754870179052928No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT15291.1580876783712546No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT11270.8536068106765231No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT11070.8384585088011635No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT10550.799072923925228No Hit
TGTAAAANGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC10480.7937710182688521No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10200.7725633956433484No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC9910.7505983579240767No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT9890.7490835277365407No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT9630.729390735298573No Hit
TGTAAAANGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT9450.7157572636107492No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT9370.7096979428606053No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGTATGTGTG9300.7043960372042294No Hit
TGTAAAANGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG9200.6968218862665495No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC9110.6900051504226375No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG8640.6544066410155421No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG8520.6453176598903263No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG8420.6377435089526464No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG8360.6331990183900384No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG8350.6324416032962705No Hit
TGTAAAANGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGTATGTGTG8240.6241100372648226No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT8160.6180507165146787No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGGTCATAGCTGTTTC8040.6089617353894629No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC7780.5892689429514951No Hit
TGTAAAANGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG7620.5771503014512073No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7520.5695761505135275No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGGTCATAGCTGTTTC7480.5665464901384555No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA7480.5665464901384555No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC6950.5264034901687521No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC6910.5233738297936801No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC6660.5044384524494804No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT6650.5036810373557125No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA6630.5021662071681764No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT6630.5021662071681764No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA6570.49762171660556853No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC6530.4945920562304966No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT6070.4597509619171691No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG6050.45823613172963307No Hit
TGTAAAANGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT5910.4476323204168813No Hit
TGTAAAANGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5840.44233041476050533No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC5620.42566728269760956No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT5580.4226376223225376No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT5550.4203653770412337No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC5410.4097615657284818No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC5370.40673190535340986No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC5330.40370224497833795No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC5300.40142999969703397No Hit
TGTAAAANGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG5290.400672584603266No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5200.3938558487593541No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC5180.3923410185718181No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT5150.39006877329051415No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT5050.38249462235283427No Hit
TGTAAAANGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT5010.3794649619777623No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC4980.3771927166964583No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC4890.37037598085254647No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC4850.36734632047747445No Hit
TGTAAAACGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT4820.3650740751961705No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4650.3521980186021147No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG4490.3400793771018269No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT4450.33704971672675493No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG4400.333262641257915No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT4350.32947556578907505No Hit
TGTAAAANGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG4350.32947556578907505No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC4300.32568849032023506No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA4170.31584209410125125No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCCTCACCTTGTCCTTGGTCA4160.31508467900748327No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC4160.31508467900748327No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4100.3105401884448753No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG3950.29917896203835553No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCCTCACCTTGTCCTTGGTCA3910.29614930166328357No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT3870.29311964128821155No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA3770.28554549035053167No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT3750.2840306601629957No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA3740.28327324506922774No Hit
TGTAAAANGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA3710.28100099978792376No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC3670.2779713394128518No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC3660.2772139243190838No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT3580.27115460356893994No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT3550.26888235828763596No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA3490.26433786772502804No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT3350.2537340564122762No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3340.25297664131850817No Hit
TGTAAAANGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT3300.24994698094343623No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT3250.24615990547459632No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT3190.24161541491198837No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT3150.2385857545369164No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCGGTCATAGCT3090.2340412639743085No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG3090.2340412639743085No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC3050.23101160359923653No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCGGTCATAGCT3040.23025418850546853No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG3000.2272245281303966No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT2960.22419486775532463No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2960.22419486775532463No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT2920.22116520738025267No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC2840.21510588663010877No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC2830.21434847153634076No Hit
TGTAAAANGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA2810.21283364134880478No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2800.2120762262550368No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT2790.2113188111612688No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT2700.20450207531735692No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2700.20450207531735692No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT2660.201472414942285No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT2630.199200169660981No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2540.1923834338170691No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC2520.19086860362953312No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC2410.18253703759808526No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2400.18177962250431726No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2330.17647771684794136No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC2310.17496288666040538No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC2280.17269064137910142No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC2270.17193322628533342No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2200.1666313206289575No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2180.16511649044142152No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2170.1643590753476535No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA2160.16360166025388553No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG2140.16208683006634955No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2080.15754233950374164No Hit
TGTAAAANGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA2020.1529978489411337No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC2010.1522404338473657No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA1960.14845335837852577No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA1880.14239403762838188No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC1880.14239403762838188No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1880.14239403762838188No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA1870.14163662253461387No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG1850.1401217923470779No Hit
TGTAAAANGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA1830.1386069621595419No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1820.13784954706577393No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1770.134062471596934No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1770.134062471596934No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC1760.13330505650316601No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA1740.13179022631563003No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1730.13103281122186203No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG1710.12951798103432607No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1680.1272457357530221No Hit
TGTAAAANGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA1650.12497349047171812No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1620.12270124519041414No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG1620.12270124519041414No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1600.12118641500287818No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA1590.12042899990911018No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1590.12042899990911018No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG1560.11815675462780621No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA1530.11588450934650225No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1520.11512709425273426No Hit
TGTAAAANGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1510.11436967915896627No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1500.1136122640651983No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1490.1128548489714303No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG1480.11209743387766231No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1440.10906777350259036No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG1410.10679552822128639No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1380.10452328293998243No Hit
TGTAAAANGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG1370.10376586784621444No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG1340.10149362256491049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)