FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003245523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23023
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT2801.2161751292186074No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG2731.185770750988142No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC2701.1727403031750858No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA2321.0076879642097034No Hit
TGTAAAACGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATGGCT2020.8773834860791382No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT1810.7861703513877426No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT1290.5603092559614299No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT1230.5342483603353169No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG1200.5212179125222604No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC1140.49515701689614733No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG1140.49515701689614733No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1130.4908135342917952No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC1120.486470051687443No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT1090.4734396038743865No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG1080.4690961212700343No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC950.4126308474134561No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA950.4126308474134561No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG950.4126308474134561No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG930.4039438822047518No Hit
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA910.3952569169960474No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA910.3952569169960474No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG910.3952569169960474No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA900.3909134343916953No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT870.3778829865786387No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT840.36485253876558227No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTAGGGTTGGGTCA820.3561655735568779No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG810.35182209095252576No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG800.34747860834817357No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA800.34747860834817357No Hit
TGTAAAACGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG780.33879164313946925No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT770.33444816053511706No Hit
TGTAAAANGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT750.3257611953264127No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT750.3257611953264127No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT730.3170742301177084No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC730.3170742301177084No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG720.3127307475133562No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG720.3127307475133562No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC700.30404378230465184No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT680.2953568170959475No Hit
TGTAAAACGACGGCCAGTAGGAGGGCGGAGGAGGAAAGCAGGTTTGCTCT660.2866698518872432No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC650.282326369282891No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGTGGGGATTCAGAG630.2736394040741867No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA630.2736394040741867No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC630.2736394040741867No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT620.2692959214698345No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACTCATCCCCCTCT610.26495243886548236No Hit
TGTAAAACGACGGCCAGTTGGATGGTGAGGGAAGGGCTAAGACAGGATTC590.256265473656778No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT590.256265473656778No Hit
TGTAAAACGACGGCCAGTTGGCCCACTGTGCTAGCCTACAGGAACCATGG590.256265473656778No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC580.25192199105242585No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTGCACGCCACCA560.2432350258437215No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG560.2432350258437215No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG560.2432350258437215No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA560.2432350258437215No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC530.230204578030665No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT530.230204578030665No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGACGGCACTGGTG530.230204578030665No Hit
TGTAAAACGACGGCCAGTTGCCCATCAGCAATCCTGCCACAAGGACACAG520.2258610954263128No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG510.22151761282196064No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC510.22151761282196064No Hit
TGTAAAANGACGGCCAGTGGTCCCACTCTGGCTTTCCCTCCTCCATGGCT500.2171741302176085No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT490.2128306476132563No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCAGCCA490.2128306476132563No Hit
TGTAAAACGACGGCCAGTAGTCAGCCACGTTGGAACCAGTGCTCACGCAA460.1998001998001998No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC450.19545671719584765No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC440.19111323459149546No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT440.19111323459149546No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGACACCGTAACTGT440.19111323459149546No Hit
TGTAAAACGACGGCCAGTCTGTCTGGCATGGGCCTCTCCACCGTGCCTGA440.19111323459149546No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCTGGAGGGCGGCCA430.1867697519871433No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC420.18242626938279113No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC420.18242626938279113No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG410.17808278677843895No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC410.17808278677843895No Hit
TGTAAAACGACGGCCAGTGCAGAAGATGGACTGTGGAGGGGGCTACATTA410.17808278677843895No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGACATTCCTGCAA410.17808278677843895No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG400.17373930417408678No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGTT390.16939582156973462No Hit
TGTAAAANGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT390.16939582156973462No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC380.16505233896538246No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC380.16505233896538246No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC380.16505233896538246No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC370.16070885636103027No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG370.16070885636103027No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC370.16070885636103027No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCATCACCAGG370.16070885636103027No Hit
TGTAAAACGACGGCCAGTGGCGCTGACGAACAGAGGAAGCGAGTACAACG360.1563653737566781No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG360.1563653737566781No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA360.1563653737566781No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC360.1563653737566781No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG350.15202189115232592No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCAGGTCATA350.15202189115232592No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG340.14767840854797376No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGTAAAACGACGG340.14767840854797376No Hit
TGTAAAACGACGGCCAGTCCCGGGCGTCAGAGTGAGAACTGTAAAACGAC340.14767840854797376No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCGGTG330.1433349259436216No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG330.1433349259436216No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG330.1433349259436216No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT320.13899144333926944No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC320.13899144333926944No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT310.13464796073491725No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGACTGCCCTCCCT310.13464796073491725No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC310.13464796073491725No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT310.13464796073491725No Hit
TGTAAAANGACGGCCAGTAGTCAGCCACGTTGGAACCAGTACTCACGCAA310.13464796073491725No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGACCAACAATGGCCTG300.1303044781305651No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG300.1303044781305651No Hit
TGTAAAACGACGGCCAGTAGTACGAAAAGTAGGTGGCCTTACTGGTAGCA300.1303044781305651No Hit
TGTAAAACGACGGCCAGTAGGTCCCACCAGGATGCCCGTGACCGAGAGCT290.12596099552621293No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC290.12596099552621293No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG290.12596099552621293No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC280.12161751292186075No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT280.12161751292186075No Hit
TGTAAAACGACGGCCAGTACATGCGTGGCCCTGTCTACGGCCCCGCCCTC280.12161751292186075No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA270.11727403031750858No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGCTCCACA270.11727403031750858No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCCGCAGTGGAGGAT260.1129305477131564No Hit
TGTAAAANGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG260.1129305477131564No Hit
TGTAAAANGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG260.1129305477131564No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTTGGGAAGGGA260.1129305477131564No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG260.1129305477131564No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC260.1129305477131564No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCCTAAGGGAG260.1129305477131564No Hit
TGTAAAACGACGGCCAGTTGTCCAACGTTGTAGTGTTGAGTGCATGGCAT260.1129305477131564No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC260.1129305477131564No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTTAACCACAGT260.1129305477131564No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGAAGCCCAGTCAGG250.10858706510880425No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC250.10858706510880425No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG250.10858706510880425No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC250.10858706510880425No Hit
TGTAAAANGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG250.10858706510880425No Hit
TGTAAAANGACGGCCAGTGGGCAGGGGAGTCAGCAGAGGCCTCGCTCGGG250.10858706510880425No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG240.10424358250445208No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT240.10424358250445208No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT240.10424358250445208No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA240.10424358250445208No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA240.10424358250445208No Hit
TGTAAAANGACGGCCAGTACCGACTGGAGAGGAAGGCACTGGTGGTAGCT240.10424358250445208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)