FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003245524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23023
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC820.3561655735568779No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA810.35182209095252576No Hit
NNGGANNNNGNTATGNNNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG590.256265473656778No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT590.256265473656778No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT570.24757850844807366No Hit
NNGGANNNNGNTATGANNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG560.2432350258437215No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA540.23454806063501715No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA520.2258610954263128No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC520.2258610954263128No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC520.2258610954263128No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG490.2128306476132563No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC490.2128306476132563No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA430.1867697519871433No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA430.1867697519871433No Hit
NAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC420.18242626938279113No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT410.17808278677843895No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT400.17373930417408678No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT390.16939582156973462No Hit
NAGGAANNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC380.16505233896538246No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC380.16505233896538246No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT370.16070885636103027No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA350.15202189115232592No Hit
NAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA340.14767840854797376No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA340.14767840854797376No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT330.1433349259436216No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC330.1433349259436216No Hit
NNGGAAANNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC320.13899144333926944No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT310.13464796073491725No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT310.13464796073491725No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT310.13464796073491725No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA310.13464796073491725No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAACAGTGAAGACAG300.1303044781305651No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT300.1303044781305651No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA290.12596099552621293No Hit
NAGGANNNNGNTATGNNNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG290.12596099552621293No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA290.12596099552621293No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT280.12161751292186075No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT280.12161751292186075No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT270.11727403031750858No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT270.11727403031750858No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT270.11727403031750858No Hit
NNGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT260.1129305477131564No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC260.1129305477131564No Hit
NAGGANNNNGNTATGANNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG260.1129305477131564No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT260.1129305477131564No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT260.1129305477131564No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT260.1129305477131564No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC260.1129305477131564No Hit
NAGGAANNNGNTATGANNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG260.1129305477131564No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC250.10858706510880425No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA250.10858706510880425No Hit
CAGGAAANAGCTATGANNGGCCTTCTCTGCATTATCTCGCTAATCTCGAG250.10858706510880425No Hit
NNGGAAANNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA250.10858706510880425No Hit
NAGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA250.10858706510880425No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT250.10858706510880425No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA250.10858706510880425No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT250.10858706510880425No Hit
NAGGAANNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT240.10424358250445208No Hit
NAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC240.10424358250445208No Hit
NNGGANNNNGNTATGNNNACCCAGTTAGAGCCATGGTTCCTGTAGGCTAG240.10424358250445208No Hit
NAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT240.10424358250445208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)