FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97981
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3790.38680968759249246No Hit
NNGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT3400.34700605219379266No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3300.33679999183515175No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3090.3153672650820057No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3090.3153672650820057No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3060.31230544697441337No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3000.30618181075922885No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA2850.2908727202212674No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG2780.2837284779702187No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2770.28270787193435465No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2540.25923393310948045No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2500.25515150896602407No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2430.24800726671497536No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2390.24392484257151897No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG2380.24290423653565485No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2310.23575999428460617No Hit
NNGGANNNNGNTATGANNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT2270.23167757014114979No Hit
NNGGANNNNGNTATGNNNGGGCATGGTGTGGGACTAGCTAATCTAGGGAC2230.22759514599769343No Hit
NNGGANNNNGNTATGANNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA2220.22657453996182933No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC2170.22147150978250887No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2160.22045090374664475No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2110.21534787356732427No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC2100.2143272675314602No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2070.21126544942386788No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2070.21126544942386788No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2020.20616241924454742No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2020.20616241924454742No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2020.20616241924454742No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2000.2041212071728192No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2000.2041212071728192No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1990.20310060113695513No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1910.19493575285004236No Hit
NNGGANNNNGNTATGANNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1870.19085332870658597No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1840.18779151059899368No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1810.1847296924914014No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1800.1837090864555373No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1770.180647268347945No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1740.17758545024035274No Hit
NNGGANNNNGNTATGNNNACAGCTTTCTGAGGGTTGTTCCAGGTGCGGTG1740.17758545024035274No Hit
NNGGANNNNGNTATGNNNTCCCACTTGCTCTTTGAAGGCAGGCACTACGT1740.17758545024035274No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1720.17554423816862455No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1710.17452363213276043No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1690.17248242006103223No Hit
NNGGANNNNGNTATGNNNCACCGCTGTCCCTTCCCAAGGACTATGCTGAA1680.17146181402516814No Hit
NNGGANNNNGNTATGANNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG1680.17146181402516814No Hit
NNGGANNNNGNTATGNNNGCATCCTGCTTAGTGCTGCCCACAGTGTCATT1670.17044120798930404No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1670.17044120798930404No Hit
NNGGANNNNGNTATGANNGCATCCTGCTTAGTGCTGCCCACAGTGTCATT1640.16737938988171175No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1630.16635878384584768No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1620.16533817780998356No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1570.1602351476306631No Hit
NNGGANNNNGNTATGNNNACAGCTTTCTGAGGGTTGTTCCAGGCGCGGTG1510.1541115114154785No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1500.15309090537961442No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1460.14900848123615804No Hit
NNGGANNNNGNTATGNNNCTGGCCTCCTTCAATGCATTTGCTCAAATGTC1430.14594666312856575No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1430.14594666312856575No Hit
NNGGANNNNGNTATGANNGGGCATGGTGTGGGACTAGCTAATCTAGGGAC1420.14492605709270165No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1410.14390545105683755No Hit
NNGGANNNNGNTATGANNCACCGCTGTCCCTTCCCAAGGACTATGCTGAA1410.14390545105683755No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1380.14084363294924526No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1360.13880242087751707No Hit
NNGGANNNNGNTATGANNACAGCTTTCTGAGGGTTGTTCCAGGTGCGGTG1340.13676120880578888No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1340.13676120880578888No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1340.13676120880578888No Hit
NNGGANNNNGNTATGNNNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1340.13676120880578888No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1340.13676120880578888No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1310.13369939069819659No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG1290.1316581786264684No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1290.1316581786264684No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1280.1306375725906043No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1280.1306375725906043No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1280.1306375725906043No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1280.1306375725906043No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1270.12961696655474023No Hit
NNGGANNNNGNTATGNNNTGCTTCAGTCGCCTGCACAAGGGGACATGGCC1260.1285963605188761No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1260.1285963605188761No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1240.1265551484471479No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1240.1265551484471479No Hit
NAGGANNNNGNTATGNNNGCTAATGCTTACTCTTTGCGTGAGTACTGGTT1230.1255345424112838No Hit
NNGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTAGGGTCTGGGGC1220.12451393637541973No Hit
NAGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA1200.12247272430369154No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1190.12145211826782742No Hit
NNGGANNNNGNTATGANNACAGCTTTCTGAGGGTTGTTCCAGGCGCGGTG1150.11736969412437105No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1140.11634908808850697No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1130.11532848205264286No Hit
NAGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1130.11532848205264286No Hit
NNGGANNNNGNTATGANNTGCTTCAGTCGCCTGCACAAGGGGACATGGCC1120.11430787601677878No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1110.11328726998091466No Hit
NAGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1110.11328726998091466No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1110.11328726998091466No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1090.11124605790918647No Hit
NNGGANNNNGNTATGANNCTGGCCTCCTTCAATGCATTTGCTCAAATGTC1090.11124605790918647No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1090.11124605790918647No Hit
NAGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1080.11022545187332237No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1080.11022545187332237No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1070.10920484583745828No Hit
NNGGANNNNGNTATGNNNACTCCCTTGAGCCTTTGGGGAAAGGGAGACAG1060.10818423980159418No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT1050.1071636337657301No Hit
NNGGANNNNGNTATGANNACCGTTAAGCCACACCAAATGGTGAGCCCTGC1040.10614302772986599No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1030.1051224216940019No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1020.1041018156581378No Hit
NNGGANNNNGNTATGNNNGTGCAGTCCTCAGGCCACACCTCCAACCCCGT1010.10308120962227371No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTCTAAGGGGACAG1010.10308120962227371No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT1000.1020606035864096No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1000.1020606035864096No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT990.10103999755054552No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT980.10001939151468142No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC980.10001939151468142No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG980.10001939151468142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)