FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78510
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4540.5782702840402496No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4270.5438797605400586No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3880.49420455992867146No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3650.46490892879887913No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3380.43051840529868807No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3370.4292446822060884No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT3320.42287606674309003No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3100.39485415870589735No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3030.38593809705769966No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3020.3846643739651No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2790.3553687428353076No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2780.3540950197427079No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2700.3439052350019106No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2550.3247993886129156No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2550.3247993886129156No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2490.31715705005731754No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2470.3146096038721182No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2440.3107884345943192No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2430.30951471150171955No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2420.30824098840911984No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2410.3069672653165202No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2370.3018723729461215No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2300.2929563112979238No Hit
NNGGANNNNGNTATGNNNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT2190.2789453572793275No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2140.2725767418163291No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2030.25856578779773276No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1980.25219717233473443No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1950.2483760030569354No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1940.24710227996433573No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1930.24582855687173608No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1910.24328111068653677No Hit
NNGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT1830.2330913259457394No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1800.22927015666794037No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1770.22544898739014135No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1770.22544898739014135No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1750.22290154120494207No Hit
NNGGANNNNGNTATGNNNACCCCACCTGCTCAAAAGACACACTCCTAAAA1740.2216278181123424No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1710.21780664883454337No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1710.21780664883454337No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1680.21398547955674435No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1640.20889058718634568No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1620.20634314100114637No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1580.2012482486307477No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1570.199974525538148No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1550.19742707935294868No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1550.19742707935294868No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1490.18978474079735067No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1470.1872372946121513No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1470.1872372946121513No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1460.18596357151955165No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1450.184689848426952No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1400.17832123296395364No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1390.17704750987135395No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1390.17704750987135395No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1390.17704750987135395No Hit
NNGGANNNNGNTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT1370.17450006368615464No Hit
NNGGANNNNGNTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1370.17450006368615464No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1360.17322634059355496No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1320.1681314482231563No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1310.1668577251305566No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1310.1668577251305566No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1310.1668577251305566No Hit
NNGGANNNNGNTATGNNNTGGGCCTGGACACTTTGACGGCGGGGCACGTC1300.16558400203795695No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1300.16558400203795695No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1260.16048910966755828No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1240.1579416634823589No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1220.1553942172971596No Hit
NNGGANNNNGNTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1210.15412049420455992No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1210.15412049420455992No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1200.15284677111196027No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1190.15157304801936058No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1190.15157304801936058No Hit
NNGGANNNNGNTATGNNNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG1190.15157304801936058No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1180.1502993249267609No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1180.1502993249267609No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1180.1502993249267609No Hit
NAGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1110.14138326327856324No Hit
NAGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1080.13756209400076425No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1080.13756209400076425No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1080.13756209400076425No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1080.13756209400076425No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1070.13628837090816456No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1070.13628837090816456No Hit
NNGGANNNNGNTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT1060.1350146478155649No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1040.13246720163036554No Hit
NNGGANNNNGNTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA1030.1311934785377659No Hit
NAGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1020.12991975544516624No Hit
NAGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT990.12609858616736722No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA990.12609858616736722No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA970.12355113998216787No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC950.12100369379696854No Hit
NAGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT950.12100369379696854No Hit
NAGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT940.11972997070436887No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA930.1184562476117692No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG910.11590880142656985No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT910.11590880142656985No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC890.11336135524137052No Hit
NAGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC890.11336135524137052No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT880.11208763214877086No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA880.11208763214877086No Hit
NAGGAANNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC880.11208763214877086No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC860.10954018596357153No Hit
NNGGANNNNGNTATGNNNTCTGACTGTCCGTGATCCCACACAATCACCCT850.10826646287097186No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG850.10826646287097186No Hit
NAGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA850.10826646287097186No Hit
NAGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA840.10699273977837218No Hit
NNGGANNNNGNTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC840.10699273977837218No Hit
NNGGANNNNGNTATGNNNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT840.10699273977837218No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC840.10699273977837218No Hit
NAGGANNNAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT830.10571901668577251No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT820.10444529359317284No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT810.10317157050057318No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT810.10317157050057318No Hit
NNGGANNNNGNTATGNNNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG800.10189784740797352No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA790.10062412431537385No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA790.10062412431537385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)