FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167242
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC9750.5829875270566006No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8730.5219980626876024No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC7470.44665813611413396No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7150.4275241865081738No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7120.425730378732615No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC6820.4077923009770273No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6790.4059984932014685No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6680.39942119802441967No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT6350.3796893124932732No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6350.3796893124932732No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT5930.35457600363545044No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC5840.3491945803087741No Hit
NNGGANNNNGNTATGNNNCTGGCCTCCTTCAATGCATTTGCTCAAATGTC5770.3450090288324703No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5620.33603998995467643No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5600.3348441181043039No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC5170.3091328733212949No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5020.30016383444350103No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4990.29837002666794227No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4980.297772090742756No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA4970.29717415481756976No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4820.2882051159397759No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA4690.28043194891235457No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4650.27804020521160955No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4580.2738546537353057No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4500.26907116633381567No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4470.2672773585582569No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT4390.2624938711567668No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4330.2589062556056493No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4330.2589062556056493No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4280.255916575979718No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG4200.25113308857822797No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC4020.24037024192487536No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC4000.2391743700745028No Hit
NNGGANNNNGNTATGANNCTGGCCTCCTTCAATGCATTTGCTCAAATGTC3970.23738056229894405No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3890.23259707489745401No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3880.23199913897226773No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3590.2146589971418663No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3530.2110713815907487No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3520.21047344566556248No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3500.20927757381518997No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3340.19971059901220983No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3320.19851472716183732No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3310.1979167912366511No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3300.1973188553114648No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3290.1967209193862786No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3050.1823704571818084No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3050.1823704571818084No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3030.18117458533143588No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3020.18057664940624962No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3000.1793807775558771No Hit
NNGGANNNNGNTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2910.1739993542292008No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2910.1739993542292008No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2870.17160761052845577No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2780.16622618720177945No Hit
NAGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2770.1656282512765932No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2630.1572571483239856No Hit
NNGGANNNNGNTATGNNNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2530.15127778907212303No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2470.14769017352100547No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2460.14709223759581921No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2440.1458963657454467No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2420.1447004938950742No Hit
NNGGANNNNGNTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2410.14410255796988794No Hit
NAGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2390.14290668611951543No Hit
NAGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2360.14111287834395667No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2350.1405149424187704No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2340.13991700649358416No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC2270.13573145501728034No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2270.13573145501728034No Hit
NNGGANNNNGNTATGNNNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT2270.13573145501728034No Hit
NAGGANNNNGNTATGNNNCTGGCCTCCTTCAATGCATTTGCTCAAATGTC2230.13333971131653533No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG2210.13214383946616282No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2190.1309479676157903No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2190.1309479676157903No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA2180.13035003169060405No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2150.12855622391504526No Hit
NNGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2140.127958287989859No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2140.127958287989859No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2080.12437067243874146No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2080.12437067243874146No Hit
NAGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2050.1225768646631827No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2030.12138099281281019No Hit
NNGGANNNNGNTATGNNNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2020.12078305688762393No Hit
NAGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1970.11779337726169263No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1960.11719544133650638No Hit
NAGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1960.11719544133650638No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1940.11599956948613387No Hit
NNGGANNNNGNTATGNNNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1930.11540163356094761No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1910.1142057617105751No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1870.11181401800983007No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1860.11121608208464381No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1840.11002021023427129No Hit
NNGGANNNNGNTATGNNNTGACTGAAAGGCTTTTCCACGTGCACTGCTGG1810.10822640245871251No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1790.10703053060834No Hit
NAGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1790.10703053060834No Hit
NNGGANNNNGNTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1780.10643259468315375No Hit
NNGGANNNNGNTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG1770.10583465875796749No Hit
NNGGANNNNGNTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1770.10583465875796749No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1760.10523672283278124No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1750.10463878690759498No Hit
NAGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1750.10463878690759498No Hit
NNGGANNNNGNTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1750.10463878690759498No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1730.10344291505722247No Hit
NNGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1730.10344291505722247No Hit
NNGGANNNNGNTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1710.10224704320684996No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1700.10164910728166371No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1700.10164910728166371No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1700.10164910728166371No Hit
NAGGANNNAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1690.10105117135647745No Hit
NNGGANNNNGNTATGNNNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1690.10105117135647745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)