FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110152
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA57735.240939792287022No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA51624.686251724889244No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG23382.1225216065073713No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA22972.085300312295737No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG22662.057157382525964No Hit
TGTAAAANGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG22342.028106616312005No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG21101.9155348972329147No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA20781.8864841310189555No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA19581.7775437577166098No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT19351.7566635195003268No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA18431.6731425666351951No Hit
TGTAAAANGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT17861.6213958893165805No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT17121.5542159924468006No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT16021.454353983586317No Hit
TGTAAAANGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG14411.3081923160723363No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG14331.3009296245188466No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT11431.0376570557048441No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC11391.0340257099280994No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC11161.0131454717118162No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC11031.0013435979373957No Hit
TGTAAAANGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT10990.9977122521606507No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC10910.990449560607161No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA10530.9559517757280848No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT10420.9459655748420365No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT9920.9005737526327258No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG9680.8787856779722565No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA9510.8633524584210909No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT9450.8579054397559736No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG9270.8415643837606217No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT9050.821591981988525No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC8850.8034352531048006No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8590.779831505555959No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC8550.7762001597792142No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT8410.7634904495606072No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT7500.6808773331396616No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT6990.6345776744861645No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT6960.6318541651536059No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT6730.610973926937323No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT6700.6082504176047643No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT6630.6018955624954608No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG5660.5138354274093979No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT5640.5120197545210254No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT5380.4884160069721839No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG5090.46208875009078365No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4930.4475633669838042No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4750.4312223109884523No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG4550.413065582104728No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT4500.4085263998837969No Hit
TGTAAAANGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG4500.4085263998837969No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4460.40489505410705207No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4320.39218534388844506No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4260.38673832522332774No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG4080.3703972692279759No Hit
TGTAAAANGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC4030.3658580870070448No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC4030.3658580870070448No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA3890.3531483767884378No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3870.3513327039000653No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTACACTTAG3780.3431621759023894No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC3300.2995860265814511No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT3230.2932311714721476No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT3230.2932311714721476No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC3140.28506064347447163No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2970.26962742392330596No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG2930.2659960781465611No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2910.2641804052581887No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT2710.24602367637446437No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG2640.23966882126516087No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2600.236037475488416No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2530.22968262037911252No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2430.22060425593725033No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2420.21969641949306412No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT2410.21878858304887788No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2320.21061805505120196No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2100.19064565327910524No Hit
TGTAAAANGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA2080.18882998039073282No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1970.1788437795046844No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1950.177028106616312No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT1950.177028106616312No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT1940.17612027017212578No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1920.17430459728375336No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1910.17339676083956715No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA1890.1715810879511947No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG1790.16250272350933256No Hit
TGTAAAANGACGGCCAGTCCTGCGCTATGGTTGGGGACCAACGTGGTGCA1780.16159488706514633No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG1780.16159488706514633No Hit
TGTAAAANGACGGCCAGTTCCAAGGTGCTGTTTCAAACTCCAGGTCATAG1600.14525383106979448No Hit
TGTAAAACGACGGCCAGTTCCAAGGTGCTGTTTCAAACTCCAGGTCATAG1590.14434599462560824No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG1510.13708330307211852No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1500.1361754666279323No Hit
TGTAAAACGACGGCCAGTAGCCCCTCGTCACCTACTCCCCGAAGTTACCA1480.1343597937395599No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCCCAGCGGCAGCAG1380.12528142929769773No Hit
TGTAAAACGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA1380.12528142929769773No Hit
TGTAAAANGACGGCCAGTAGTGGAAGCTATTAGCACCAATGACATAAATA1230.11166388263490451No Hit
TGTAAAANGACGGCCAGTGAGAGGGCAGAAGCATGTTCCGGGGGTCATAG1190.10803253685815963No Hit
TGTAAAANGACGGCCAGTGGTCCCACTCTGGCTTTCCCACCTGATAATAA1120.10167768174885612No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1110.10076984530466991No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)