FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103089
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC25142.4386694991706195No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC24642.390167719155293No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG24542.3804673631522277No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA22962.227201738303796No Hit
CAGGAAACAGCTATGACCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT22312.1641494242838712No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC21702.1049772526651727No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT21312.067145864253218No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC20101.949771556616128No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA19181.8605282813879271No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA17251.6733114105287665No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA17241.67234137492846No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG16661.616079310110681No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG15771.5297461416833997No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA15551.5084053584766561No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT14731.4288624392515206No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT14111.3687202320325156No Hit
CAGGAAACAGCTATGACCTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG13691.3279787368196412No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG13611.3202184520171891No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC12381.2009040731794858No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT11971.161132613566918No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT11941.1582225067659984No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT11441.1097207267506717No Hit
CAGGAAACAGCTATGACCTCTGACTGTCCGTGATCCCACACAATCACCCT11391.1048705487491393No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT10981.0650990891365713No Hit
CAGGAAACAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC10901.0573388043341192No Hit
CAGGAAACAGCTATGACCCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA10801.0476384483310537No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT10661.0340579499267624No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT9450.916683642289672No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG9390.9108634286878329No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA9060.8788522538777174No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC8960.869151897874652No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT8780.8516912570691345No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA8030.7789385870461446No Hit
CAGGAAACAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG7730.7498375190369486No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG7280.7061859170231548No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA6930.6722346710124261No Hit
CAGGAAACAGCTATGACCTGACTTTTGCAAGTCACAGAGAACTGTTGTAT6860.6654444218102805No Hit
CAGGAAACAGCTATGACCAGCAAAGGTCTTGGCAGGGGTTCTCCTTCCCT6850.6644743862099739No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT6230.6043321789909689No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT5860.5684408617796274No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT5840.5665007905790143No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA5600.5432199361716574No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT5480.5315795089679791No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA5050.4898679781547983No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5040.48889794255449176No Hit
CAGGAAACAGCTATGACCGGGGAATGGGAAGGGAGATGAAAGAGTAGGTG4930.47822755095111985No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA4510.43748605573824556No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA4460.43263587773671297No Hit
CAGGAAACAGCTATGACCCCACCCTCATGCCTAACCCCTATTTTCCAAAG4380.4248755929342607No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT3990.387044204522306No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT3920.3802539553201602No Hit
CAGGAAACAGCTATGACCTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT3870.37540377731862756No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA3860.37443374171832106No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC3820.3705535993170949No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC3780.36667345691586883No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTCCATTCT3500.3395124601072859No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3430.33272221090514026No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA3350.324961926102688No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTATTCCCAACCA3320.3220518193017684No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA3210.3113814276983965No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCAGAGAGTGATAT3190.3094413564977835No Hit
CAGGAAACAGCTATGACCTCAGTGCCACCAAAGAAGTGCAAGAAGAAAAG3150.30556121409655734No Hit
CAGGAAACAGCTATGACCGCCATGCTTGAATCTTCGCAGTTTAGCTGTAA3090.29974100049471814No Hit
CAGGAAACAGCTATGACCGCATCACTGAGGGAGGATCCTCGGGTCTTACA3030.293920786892879No Hit
CAGGAAACAGCTATGACCAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA2990.29004064449165284No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT2950.2861605020904267No Hit
CAGGAAACAGCTATGACCGATGCTCCTTCCTTGGCCCCCACTCCTCCCCC2880.279370252888281No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT2750.26675979008429607No Hit
CAGGAAACAGCTATGACCGCTCCAGGAGAGAGCAGCCTTCCTCACACCCG2640.25608939848092427No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGCCTTGTGCTGCC2600.25220925607969813No Hit
CAGGAAACAGCTATGACCAAACCCTCGACCCTGAGGCTGGAGAACCTCAG2410.23377857967387403No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCATTGCACTC2400.23280854407356752No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2390.23183850847326098No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2350.22795836607203487No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2310.22407822367080873No Hit
CAGGAAACAGCTATGACCGGCGGGCCCTGATATAGAGCAGGCGCCGCGGG2110.2046775116646781No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGGCCACTGCAGCT2080.2017674048637585No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2020.19594719126191934No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT1880.18236669285762788No Hit
CAGGAAACAGCTATGACCAGCGTCTCATTCCATAAAGGCCATACCAGAAA1680.16296598085149724No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCATAAGAGT1670.1619959452511907No Hit
CAGGAAACAGCTATGACCACCCCTTGCATTGGTAGGGCTTCTCTCCTGTG1580.15326562484843193No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA1530.14841544684689928No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGTACTGTAA1500.14550534004597968No Hit
CAGGAAACAGCTATGACCGCTGAGGCAGGACAATCGCTTGAACTTGGGAG1450.14065516204444703No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1450.14065516204444703No Hit
CAGGAAACAGCTATGACCTCCAGCCAGCCAGTCCTTGTAACTCAGCATGA1440.1396851264441405No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCGCAGTGGCTGAT1370.13289487724199478No Hit
CAGGAAACAGCTATGACCTGATTAATGGCAGGGTTGTAGCCTGAATATAT1290.12513459243954253No Hit
CAGGAAACAGCTATGACCCCCATCCACTAGTCGGGGAGTTACACAAACAG1270.12319452123892946No Hit
CAGGAAACAGCTATGACCTGCTGTAAATGTTGGCGGGGAGGGGTCTCATT1230.11931437883770334No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT1210.11737430763709028No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCATCTGTAAAA1100.10670391603371844No Hit
CAGGAAACAGCTATGACCATCCACTGTTGGCCAACACAATAACCCCCTCC1070.10379380923279885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)