FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146850
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA104417.109976166155942No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG83385.677902621722847No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG41442.821927136533878No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG38782.640789921688798No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG38722.636704119850187No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA37792.573374191351719No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC34462.3466121893088188No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG34312.3363976847122916No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC31362.1355124276472592No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC31142.1205311542390195No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG31052.1144024514811033No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT30452.073544433094995No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT28541.9434797412325504No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC25631.7453183520599251No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA24251.651344909771876No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC22711.546475995914198No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC19801.348314606741573No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG15691.0684371807967314No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA15651.0657133129043241No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC15611.0629894450119168No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC15491.0548178413346951No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC14380.979230507320395No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT13420.9138576779026217No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC13260.902962206332993No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT13080.8907048008171603No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT12340.8403132448076268No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA8890.6053796390875043No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT8650.589036431733061No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT8240.561116785835887No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA8160.5556690500510725No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGAGGGAGGGGTG7070.48144364998297584No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG6080.4140279196458972No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT5750.3915560095335376No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT5540.37725570309839973No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG4980.3391215526046987No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4630.3152877085461355No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTGCGGCCCCTG4600.3132448076268301No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4340.29553966632618317No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG4340.29553966632618317No Hit
TGTAAAACGACGGCTAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA3700.2519577800476677No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA3490.2376574736125298No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT3430.23357167177391897No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGCGGCCTTACTGGTAGCA3390.23084780388151174No Hit
TGNAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCTTTACTGGTAGCA3210.21859039836567926No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG3130.21314266258086484No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA3020.20565202587674497No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG2930.19952332311882873No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGACCTTACTGGTAGCA2870.1954375212802179No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC2770.18862785154919987No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA2740.18658495062989444No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG2730.18590398365679264No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCACGAT2520.17160367722165473No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT2440.16615594143684032No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG2380.1620701395982295No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG2290.15594143684031325No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA1890.12870275791624106No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA1750.1191692202928158No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCCACAAGGAGACGATG1740.11848825331971401No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG1660.11304051753489956No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA1610.10963568266939053No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1580.10759278175008513No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG1540.1048689138576779No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT1540.1048689138576779No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1500.10214504596527069No Hit
TGNAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGGGTAGCCACAG1490.10146407899216889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)