FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66413
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA15202.2887085359794015No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA15052.266122596479605No Hit
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG12231.8415069338834265No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG11981.8038637013837653No Hit
TGTAAAANGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA9551.4379714814870583No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG9271.3958110610874377No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA9061.3641907457877223No Hit
TGTAAAANGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG8971.3506391820878443No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG8431.269329799888576No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG7931.1940433348892536No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT7551.1368256214897685No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT7271.094665201090148No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT6951.0464818634905817No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT6270.9440922710915031No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA5730.862782888892235No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA5510.8296568442925331No Hit
TGTAAAANGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG5340.8040594461927635No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT5270.7935193410928583No Hit
TGTAAAANGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT5260.7920136117928719No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA5220.785990694592926No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT5190.7814735066929668No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT4970.7483474620932649No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG4930.7423245448933191No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC4920.7408188155933326No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA4860.7317844397934139No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGGTCATAGCTGTTTC4830.7272672518934546No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC4800.7227500639934953No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG4510.6790839142938883No Hit
TGTAAAANGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC4200.6324063059943084No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG4090.6158432836944574No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC4050.6098203664945117No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGCCTCACAGGGTCATAGCTG4000.6022917199945793No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG3840.5782000511947962No Hit
TGTAAAANGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT3780.5691656753948775No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC3740.5631427581949318No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGGTCATAGCTGTTTC3720.5601312995949588No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC3610.5435682772951079No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT3590.5405568186951349No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG3540.5330281721952028No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGCGCTCATATAGAAG3490.5254995256952705No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT3490.5254995256952705No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGCGCTCATATAGAAG3430.5164651498953519No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGCCTCACAGGGTCATAGCTG3350.5044193154954602No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT3170.47731618809570414No Hit
TGTAAAANGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG3130.4712932708957584No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTACTGGGGCACATGGG3110.46828181229578547No Hit
TGTAAAANGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT3090.4652703536958126No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTACTGGGGCACATGGG3030.4562359778958938No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC3010.453224519295921No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT2990.4502130606959481No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT2940.4426844141960158No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT2860.43063857979612424No Hit
TGTAAAANGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT2840.4276271211961513No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC2710.4080526402963275No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG2700.4065469109963411No Hit
TGTAAAACGACGGCCAGTCCCTCCAGCTGCTCTCTGCTGGGGCACATGGG2530.3809495128965714No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT2460.3704094077966663No Hit
TGTAAAANGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT2400.36137503199674764No Hit
TGTAAAANGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG2390.35986930269676115No Hit
TGTAAAACGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT2370.3568578440967883No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC2330.3508349268968425No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG2320.34932919759685604No Hit
TGTAAAANGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT2250.33878909249695094No Hit
TGTAAAANGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG2160.32523752879707285No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC2000.30114585999728966No Hit
TGTAAAACGACGGCCAGTTCACAGTGCTGTAGGGGCCTACACGTTCCAGG1980.2981344013973168No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT1920.2891000255973981No Hit
TGTAAAANGACGGCCAGTCCCTCCAGCTGCTCTCTGCTGGGGCACATGGG1870.2815713790974659No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC1860.2800656497974794No Hit
TGTAAAANGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT1840.2770541911975065No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG1840.2770541911975065No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG1830.2755484618975201No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG1770.2665140860976014No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT1770.2665140860976014No Hit
TGTAAAANGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA1770.2665140860976014No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA1730.26049116889765556No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC1610.24242241729781822No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT1570.2363995000978724No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC1500.22585939499796728No Hit
TGTAAAACGACGGCCAGTCTCTCATCCCCCTCTCCCAGCAGGTCATAGCT1490.22435366569798082No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA1460.21983647779802148No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC1440.21682501919804859No Hit
TGTAAAANGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG1440.21682501919804859No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1430.21531928989806212No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT1430.21531928989806212No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG1420.21381356059807566No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC1360.20477918479815696No Hit
TGTAAAANGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT1340.2017677261981841No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG1290.19423907969825183No Hit
TGTAAAANGACGGCCAGTCTCTCATCCCCCTCTCCCAGCAGGTCATAGCT1270.19122762109827895No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACACACAAAGC1230.18520470389833316No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1220.18369897459834672No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACACTCTACCCGGCACCCAGGGTC1180.17767605739840092No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT1170.17617032809841446No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT1140.17165314019845512No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1140.17165314019845512No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA1130.17014741089846866No Hit
TGTAAAANGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACACACAAAGC1110.16713595229849576No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC1090.1641244936985229No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACACTCTACCCGGCACCCAGGGTC1050.1581015764985771No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGCTCATATAGAAG1040.15659584719859065No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCACCCAGGGTC1030.1550901178986042No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC990.1490672006986584No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC990.1490672006986584No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC990.1490672006986584No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG950.1430442834987126No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC950.1430442834987126No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT950.1430442834987126No Hit
TGTAAAACGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA940.14153855419872616No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCACCCAGGGTC930.1400328248987397No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT920.13852709559875326No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT920.13852709559875326No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG910.13702136629876682No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGGGCTCATATAGAAG910.13702136629876682No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG900.13551563699878036No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC890.1340099076987939No Hit
TGTAAAACGACGGCCAGTGTGTTCAGGGTGGCTGCGGTCATAGCTGTTTC880.13250417839880746No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG880.13250417839880746No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT880.13250417839880746No Hit
TGTAAAANGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCAGGGA860.1294927197988346No Hit
TGTAAAANGACGGCCAGTGGATGCCAAGGCAGTACCTGAAGCACCGGGGA860.1294927197988346No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT850.12798699049884812No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG840.12648126119886166No Hit
TGTAAAANGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT820.12346980259888878No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG820.12346980259888878No Hit
TGTAAAACGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA810.12196407329890231No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC780.11744688539894299No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT770.11594115609895653No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG770.11594115609895653No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG760.11443542679897008No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG760.11443542679897008No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC750.11292969749898364No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT740.11142396819899718No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG730.10991823889901074No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG710.10690678029903783No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT700.1054010509990514No Hit
TGTAAAANGACGGCCAGTAGGCTTTGGGAGGCCATGACTAGTTAGAGACA700.1054010509990514No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT690.10389532169906494No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA680.10238959239907848No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA670.10088386309909204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)