FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86831
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG42174.85656044500236No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG23032.652278564107289No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG15051.733251949188654No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG14911.7171286752427128No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT10071.1597240616830393No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT9271.067591067706234No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT9001.0364961822390621No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8981.034192857389642No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8520.9812163858529788No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT8430.9708514240305881No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC8130.936301551289286No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT8100.932846564015156No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8100.932846564015156No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT7890.9086616530962445No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7850.9040550033974042No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7780.8959933664244336No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT6890.7934954106252377No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA6670.768158837281616No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC6270.7220923402932132No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC6200.7140307033202428No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC5310.6115327475210467No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC4790.551646301436123No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT4780.5504946390114129No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC4730.5447363268878627No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG4330.49866982989945985No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG4280.4929115177759095No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4220.4860015432276491No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4210.48484988080293906No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4190.4825465559535189No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3990.4595133074593175No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT3990.4595133074593175No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3930.4526033329110571No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3920.45145167048634705No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3910.45030000806163695No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3880.44684502078750676No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3770.434176734115696No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3630.4180534601697551No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3620.416901797745045No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3570.4111434856214946No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3390.3904135619767134No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3300.3800486001543228No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3260.37544195045548245No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3220.3708353007566422No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3210.36968363833193213No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3170.36507698863309185No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3100.3570153516601214No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3080.35471202681070124No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2980.34319540256360054No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2870.33052711589178985No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2840.32707212861765955No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2810.32361714134352937No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2810.32361714134352937No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2760.31785882921997904No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2650.30519054254816824No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT2640.3040388801234582No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2610.300583892849328No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2610.300583892849328No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC2530.29137059345164745No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2500.28791560617751727No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2490.28676394375280717No Hit
NAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2440.28100563162925685No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2420.27870230677983665No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2400.27639898193041657No Hit
CAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2400.27639898193041657No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2300.26488235768331586No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2200.25336573343621516No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2140.24645575888795476No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2090.2406974467644044No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2050.23609079706556416No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT2010.23148414736672385No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1940.22342251039375338No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1930.2222708479690433No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC1850.21305754857136278No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1840.2119058861466527No Hit
CAGGAAANAGCTATGANCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1840.2119058861466527No Hit
CAGGAAACAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1840.2119058861466527No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1780.20499591159839226No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC1770.20384424917368218No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1750.20154092432426207No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1740.200389261899552No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1730.19923759947484193No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC1690.19463094977600165No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1680.1934792873512916No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1670.19232762492658154No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1670.19232762492658154No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1650.1900243000771614No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1630.18772097522774123No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1630.18772097522774123No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1620.18656931280303116No Hit
CAGGAAACAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1550.1785076758300607No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1540.17735601340535062No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1530.17620435098064055No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1480.1704460388570902No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1480.1704460388570902No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1440.16583938915824994No Hit
CAGGAAANAGCTATGANNTCCCTGCTTGATAATGACCCCTCTGAATTCTT1440.16583938915824994No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1390.1600810770346996No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1390.1600810770346996No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1360.15662608976056938No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1320.1520194400617291No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1300.14971611521230896No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1290.1485644527875989No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1270.14626112793817875No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1240.14280614066404856No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1230.1416544782393385No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1190.1370478285404982No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1170.13474450369107807No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1170.13474450369107807No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1160.133592841266368No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1130.1301378539922378No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1110.12783452914281765No Hit
CAGGAAANAGCTATGANCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1100.12668286671810758No Hit
CAGGAAANAGCTATGANCGTCAACAGCATACCTGTACACCTGAAATAACT1090.1255312042933975No Hit
CAGGAAANAGCTATGANCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1060.12207621701926731No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1060.12207621701926731No Hit
NAGGAAANAGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1060.12207621701926731No Hit
CAGGAAANAGCTATGANCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1050.12092455459455724No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1040.11977289216984718No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1030.11862122974513711No Hit
NAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1020.11746956732042704No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1000.11516624247100689No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA990.11401458004629682No Hit
NAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT990.11401458004629682No Hit
CAGGAAANAGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT980.11286291762158676No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA980.11286291762158676No Hit
NAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA980.11286291762158676No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT960.11055959277216662No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC950.10940793034745655No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC940.1082562679227465No Hit
NAGGAAANAGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT940.1082562679227465No Hit
NAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC910.10480128064861628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)