FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128696
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC9760.7583763287126251No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8450.6565860632809101No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA7150.5555728227761547No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG7080.550133648287437No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC6720.5221607509168894No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT6350.49341082861938207No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6180.48020140486106794No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT5270.40949213650773914No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG5260.40871511158077956No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA5070.39395163796854604No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG4900.38074221421023186No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC4600.35743146640144213No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4370.3395598930813701No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4240.3294585690308945No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4220.3279045191769752No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4220.3279045191769752No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT4210.3271274942500155No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4110.31935724498041895No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG4110.31935724498041895No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3970.3084788960029838No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA3960.30770187107602415No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3890.30226269658730653No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC3870.3007086467333872No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC3820.2968235220985889No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG3750.2913843476098713No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3740.2906073226829117No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3470.26962764965500097No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3340.2595263256045254No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG3330.25874930067756574No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3320.2579722757506061No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3270.25408715111580776No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3210.24942500155404984No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3200.2486479766270902No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3100.2408777273574936No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3100.2408777273574936No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3050.23699260272269534No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA3050.23699260272269534No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2950.2292223534530988No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2780.21601292969478464No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2770.21523590476782492No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2760.21445887984086529No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC2750.21368185491390562No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2700.20979673027910736No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2630.20435755579038978No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2630.20435755579038978No Hit
NAGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2600.20202648100951076No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2570.1996954062286318No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC2540.19736433144775284No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2520.19581028159383354No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2520.19581028159383354No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2510.19503325666687388No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2440.18959408217815626No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA2420.18804003232423697No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2360.18337788276247902No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2320.1802697830546404No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2310.17949275812768073No Hit
NNGGANNNNGNTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC2280.17716168334680177No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC2270.1763846584198421No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2230.17327655871200348No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2190.17016845900416486No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT2190.17016845900416486No Hit
NAGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2130.1655063094424069No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2120.16472928451544724No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2100.16317523466152795No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCGGGGATCCTCG2080.16162118480760865No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2070.16084415988064898No Hit
NAGGAAANAGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2040.15851308509977No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2000.15540498539193137No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1980.15385093553801207No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1980.15385093553801207No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1960.15229688568409275No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1930.1499658109032138No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1920.14918878597625412No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1870.14530366134145584No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1860.14452663641449617No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1860.14452663641449617No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA1850.1437496114875365No Hit
NAGGAANNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1840.14297258656057688No Hit
NAGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1800.13986448685273822No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1790.13908746192577856No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1790.13908746192577856No Hit
NNGGANNNNGNTATGNNNACAGGGGCCTCTTAAAACTGCAAGCCTGACTG1770.13753341207185926No Hit
NNGGAAANNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1730.13442531236402064No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1720.13364828743706098No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1720.13364828743706098No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1720.13364828743706098No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1700.13209423758314165No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1700.13209423758314165No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1690.13131721265618201No Hit
NAGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1680.13054018772922235No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1660.12898613787530305No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1630.12665506309442406No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1620.1258780381674644No Hit
NAGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1620.1258780381674644No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCCCATCCGG1600.1243239883135451No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1600.1243239883135451No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1600.1243239883135451No Hit
NAGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1600.1243239883135451No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1590.12354696338658544No Hit
NAGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1590.12354696338658544No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1530.11888481382482749No Hit
NAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1520.11810778889786784No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1510.11733076397090819No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCGGGGATCCTCG1500.11655373904394853No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1490.11577671411698888No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1490.11577671411698888No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA1490.11577671411698888No Hit
NAGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1480.11499968919002923No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1480.11499968919002923No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1470.11422266426306955No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG1470.11422266426306955No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1460.11344563933610989No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1450.11266861440915024No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1450.11266861440915024No Hit
NAGGANNNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1450.11266861440915024No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC1450.11266861440915024No Hit
NAGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1440.11189158948219059No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1440.11189158948219059No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1430.11111456455523093No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAACGCAGCTCCCCA1420.11033753962827128No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1410.10956051470131162No Hit
NAGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1410.10956051470131162No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1410.10956051470131162No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1410.10956051470131162No Hit
NAGGAAANAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1390.10800646484739232No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1390.10800646484739232No Hit
NNGGANNNNGNTATGNNNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1390.10800646484739232No Hit
NAGGAANNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1380.10722943992043264No Hit
NAGGANNNAGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1370.10645241499347298No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1370.10645241499347298No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1370.10645241499347298No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGGCCTGGCTTGTC1370.10645241499347298No Hit
NAGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1350.10489836513955368No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1350.10489836513955368No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1350.10489836513955368No Hit
NNGGANNNNGNTATGNNNAGCAAAGGTCTTGGCAGGGGGTGGGGACTGGA1340.10412134021259402No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1330.10334431528563437No Hit
NAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1330.10334431528563437No Hit
NNGGANNNNGNTATGNNNTGCTGCCGAGAGAGGACAGTCCCCAACCATAG1320.1025672903586747No Hit
NAGGAANNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1310.10179026543171506No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1300.10101324050475538No Hit
NAGGAAANAGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1300.10101324050475538No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1300.10101324050475538No Hit
NAGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1290.10023621557779573No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1290.10023621557779573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)