FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146605
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT7460.5088503120630264No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT7150.4877050578083967No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT6110.4167661403089935No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT5700.38879983629480575No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5540.377886156679513No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT5400.3683366870161318No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5400.3683366870161318No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC5370.36629037208826437No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG5160.3519661675931926No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4490.306265134204154No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4490.306265134204154No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT4480.30558302922819824No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4440.302854609324375No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT4230.28853040482930326No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC4150.28307356502165687No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC4010.27352409535827565No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG3780.2578356809112922No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA3710.25306094607960167No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3710.25306094607960167No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3700.2523788411036459No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3550.24214726646430884No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3500.23873674158452987No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3490.23805463660857407No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3460.23600832168070665No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3420.23327990177688346No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA3390.23123358684901604No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3390.23123358684901604No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3360.22918727192114868No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3350.22850516694519285No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3330.22714095699328127No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG3290.22441253708945808No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3260.22236622216159066No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3230.2203199072337233No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3120.2128167524982095No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3080.21008833259438625No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA3040.2073599126905631No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC2890.1971283380512261No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2880.19644623307527026No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2860.19508202312335868No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2840.1937178131714471No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2820.19235360321953549No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2780.18962518331571226No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2770.1889430783397565No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2750.18757886838784488No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2710.18485044848402168No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA2680.18280413355615427No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT2670.1821220285801985No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2660.1814399236042427No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2640.1800757136523311No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2610.1780293987244637No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2560.1746188738446847No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2520.1718904539408615No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2500.17052624398894992No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2480.1691620340370383No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2470.1684799290610825No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGTGATCCTCAA2460.1677978240851267No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2440.1664336141332151No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2440.1664336141332151No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2410.16438729920534773No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2330.1589304593977013No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2310.1575662494457897No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2300.1568841444698339No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2290.1562020394938781No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2260.1541557245660107No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2230.15210940963814332No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2210.1507451996862317No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2210.1507451996862317No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2190.1493809897343201No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2180.14869888475836432No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2130.14528835987858532No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2130.14528835987858532No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2110.1439241499266737No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2100.1432420449507179No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2080.14187783499880632No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2060.1405136250468947No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2060.1405136250468947No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC2050.13983152007093894No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2050.13983152007093894No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2040.13914941509498313No Hit
NNGGANNNNGNTATGANNGGGTCCCACCCATGCTGAAATGTGATCCTCAA1990.1357388902152041No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1990.1357388902152041No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1970.13437468026329252No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1960.13369257528733672No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1950.13301047031138094No Hit
NNGGANNNNGNTATGANNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1920.13096415538351353No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCTGCCCAACACCC1910.13028205040755772No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1900.12959994543160192No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1880.12823573547969033No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1860.12687152552777872No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1860.12687152552777872No Hit
NAGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1860.12687152552777872No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1850.12618942055182295No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1830.12482521059991132No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGTTGAGCTCCTCA1780.12141468572013234No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1780.12141468572013234No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1750.11936837079226494No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1710.11663995088844173No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1710.11663995088844173No Hit
NAGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1700.11595784591248592No Hit
NNGGANNNNGNTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1680.11459363596057434No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACCCTTTGTCCA1660.11322942600866275No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGTCTGCAGCCTGG1650.11254732103270693No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1620.11050100610483952No Hit
NAGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1620.11050100610483952No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1610.10981890112888373No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1570.10709048122506054No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1570.10709048122506054No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1550.10572627127314893No Hit
NNGGANNNNGNTATGNNNACAGGGGCCTCTTAAAACTGCAAGCCTGACTG1550.10572627127314893No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1530.10436206132123733No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1530.10436206132123733No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1530.10436206132123733No Hit
NAGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1520.10367995634528154No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1520.10367995634528154No Hit
NAGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1510.10299785136932574No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1500.10231574639336995No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1500.10231574639336995No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCAGGAAACAGCT1490.10163364141741414No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1480.10095153644145835No Hit
NAGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1470.10026943146550255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)