FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144865
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT23291.6077037241569738No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT22681.565595554481759No Hit
CAGGAAANAGCTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC17991.2418458564870742No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT17851.2321816863976807No Hit
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG16631.1479653470472508No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT16241.1210437303696545No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA15831.0927415179650017No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT15651.0803161564214958No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC13940.9622752217581887No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT13670.9436371794429296No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC12800.8835812653159839No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG12460.860111137956028No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT12070.8331895212784317No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9510.6564732682152349No Hit
CAGGAAANAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC9250.6385255237635039No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT8860.6116039070859076No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC8830.6095330134953232No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT8730.6026300348600421No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8610.5943464604977048No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG8550.5902046733165361No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT8520.5881337797259517No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT8310.5736375245918613No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8260.5701860352742208No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG8180.5646636523659959No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG8020.553618886549546No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC7300.5039174403755221No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA7130.49218237669554415No Hit
CAGGAAANAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC7100.4901114831049598No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6740.46526076001794775No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT6310.43557795188623893No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6250.43143616470507024No Hit
CAGGAAANAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6180.4266040796603735No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG6120.42246229247920475No Hit
CAGGAAACAGCTATGACCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC6110.42177199461567666No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5940.41003693093569876No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT5660.39070859075691167No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG5540.38242501639457427No Hit
CAGGAAANAGCTATGANNTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT5500.3796638249404618No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC5410.3734511441687088No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG5400.3727608463051807No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5390.3720705484416526No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5390.3720705484416526No Hit
CAGGAAANAGCTATGANCTGGGGATGAGGACAGCAGGGCAGTCACCATCT5220.36033548476167465No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5210.35964518689814656No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA5210.35964518689814656No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5160.356193697580506No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT5110.35274220826286545No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC5080.3506713146722811No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA4970.3430780381734719No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC4970.3430780381734719No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG4890.33755565526524695No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC4840.3341041659476064No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4670.3223691022676285No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4590.31684671935940356No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4500.31063403858765054No Hit
CAGGAAACAGCTATGACCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4490.30994374072412245No Hit
CAGGAAANAGCTATGANCTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC4330.29889897490767264No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA4190.2892348048182791No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4120.28440271977358234No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4060.28026093259241364No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT3950.2726676560936044No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3940.2719773582300763No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3920.27059676250302006No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3870.2671452731853795No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3870.2671452731853795No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3750.2588616988230421No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT3660.25264901805128914No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3570.24643633727953612No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3530.24367514582542366No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3500.24160425223483933No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG3410.23539157146308634No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC3330.22986918855486144No Hit
CAGGAAANAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT3280.22641769923722083No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3090.21330203983018675No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3080.2126117419666586No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC3050.21054084837607429No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2980.2057087633313775No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC2830.19535429537845583No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2810.19397369965139957No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2800.19328340178787146No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2780.19190280606081522No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC2710.18707072101611846No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2700.18638042315259035No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2690.18569012528906223No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2680.1849998274255341No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2590.17878714665378112No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2550.17602595519966865No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2540.17533565733614054No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2540.17533565733614054No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2530.17464535947261242No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2520.1739550616090843No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2460.16981327442791566No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2460.16981327442791566No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2350.1622199979291064No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2280.15738791288440962No Hit
CAGGAAACAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT2270.1566976150208815No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2260.1560073171573534No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2240.15462672143029715No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC2230.15393642356676907No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2180.1504849342491285No Hit
CAGGAAANAGCTATGANCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT2180.1504849342491285No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2180.1504849342491285No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2090.1442722534773755No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2070.14289165775031926No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGGAACACACAACTGGC2060.14220135988679117No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2060.14220135988679117No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2050.14151106202326305No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC2050.14151106202326305No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2050.14151106202326305No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2030.14013046629620682No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1990.13736927484209435No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1970.13598867911503815No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT1970.13598867911503815No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1950.1346080833879819No Hit
CAGGAAANAGCTATGACCCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT1920.1325371897973976No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1870.129085700479757No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1840.1270148068891727No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1770.1221827218444759No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC1750.12080212611741967No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1750.12080212611741967No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1730.11942153039036345No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1720.11873123252683533No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1710.11804093466330721No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1680.11597004107272288No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA1660.11458944534566665No Hit
CAGGAAANAGCTATGANNCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1660.11458944534566665No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1660.11458944534566665No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1610.1111379560280261No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1590.10975736030096987No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1580.10906706243744176No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1580.10906706243744176No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1570.10837676457391364No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1540.1063058709833293No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA1540.1063058709833293No Hit
CAGGAAACAGCTATGANCAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1520.10492527525627307No Hit
NAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1510.10423497739274495No Hit
NAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1480.10216408380216065No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1480.10216408380216065No Hit
CAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1470.10147378593863253No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1470.10147378593863253No Hit
CAGGAAANAGCTATGANNGCAGCTCCCCACAACTTCAACTGGCCGTCGTT1460.10078348807510441No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1450.1000931902115763No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1450.1000931902115763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)