FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124225
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6610.5320990138860938No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT6020.4846045481988328No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC5980.48138458442342524No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5910.47574964781646206No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT5510.4435500100623868No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5410.43550010062386796No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5320.4282551821292011No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC5000.4024954719259408No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4480.360635942845643No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4480.360635942845643No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4310.346951096800161No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4270.3437311330247535No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT4050.32602133226001206No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC4000.32199637754075267No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4000.32199637754075267No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3740.3010666130006038No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3520.28335681223586234No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3450.27772187562889916No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3430.2761118937411954No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT3360.27047695713423225No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3280.2640370295834172No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3220.2592070839203059No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3220.2592070839203059No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3210.25840209297645406No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3190.2567921110887503No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3180.2559871201448984No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT3110.2503521835379352No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3100.2495471925940833No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3060.24632722881867578No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2890.2326423827731938No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2850.2294224189977863No Hit
NNGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG2830.22781243711008253No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2810.22620245522237875No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2750.22137250955926746No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2570.2068826725699336No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2520.20285771785067416No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2500.2012477359629704No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2480.19963775407526668No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2350.18917287180519218No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2320.18675789897363654No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2320.18675789897363654No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2310.18595290802978467No Hit
NNGGANNNNGNTATGNNNAGAGACCAGATGCATAAGCACAGCAGCATTCC2290.1843429261420809No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2250.18112296236667338No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2240.1803179714228215No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2230.17951298047896963No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2220.17870798953511774No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2190.17629301670356207No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2180.1754880257597102No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2130.1714630710404508No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2110.16985308915274702No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2100.16904809820889516No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT2100.16904809820889516No Hit
NNGGANNNNGNTATGANNTGACAGCACAAGGAACACTGACCCGAGGCAGG2080.16743811632119138No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2070.1666331253773395No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA2060.16582813443348762No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2060.16582813443348762No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2050.16502314348963576No Hit
NAGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2030.16341316160193198No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2030.16341316160193198No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2010.16180317971422822No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1980.15938820688267255No Hit
NAGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1980.15938820688267255No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1970.1585832159388207No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1960.15777822499496882No Hit
NNGGANNNNGNTATGNNNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1930.15536325216341315No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1900.1529482793318575No Hit
NNGGANNNNGNTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1890.15214328838800562No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1850.1489233246125981No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1840.14811833366874622No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1830.14731334272489435No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1800.14489836989333868No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1800.14489836989333868No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1790.14409337894948682No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1790.14409337894948682No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1760.14167840611793117No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1730.13926343328637553No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1720.13845844234252364No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1700.13684846045481988No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1680.13523847856711613No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1680.13523847856711613No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1680.13523847856711613No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1670.13443348762326424No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1660.13362849667941235No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1640.1320185147917086No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1620.1304085329040048No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1620.1304085329040048No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1610.12960354196015295No Hit
NNGGANNNNGNTATGNNNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1560.12557858724089355No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1540.12396860535318976No Hit
NAGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1530.12316361440933789No Hit
NAGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1510.12155363252163415No Hit
NNGGANNNNGNTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1510.12155363252163415No Hit
NAGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1480.11913865969007849No Hit
NNGGANNNNGNTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1480.11913865969007849No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1440.11591869591467095No Hit
NAGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1420.11430871402696721No Hit
NNGGANNNNGNTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC1380.11108875025155968No Hit
NNGGANNNNGNTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA1360.10947876836385591No Hit
NAGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1360.10947876836385591No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1340.10786878647615214No Hit
NAGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1340.10786878647615214No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCACTCAGCGTGTG1340.10786878647615214No Hit
NAGGANNNNGNTATGNNNTGACAGCACAAGGAACACTGACCCGAGGCAGG1330.10706379553230028No Hit
NNGGANNNNGNTATGNNNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1330.10706379553230028No Hit
NAGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1320.10625880458844839No Hit
NNGGANNNNGNTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1320.10625880458844839No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1310.10545381364459651No Hit
NAGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1300.10464882270074462No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1300.10464882270074462No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1270.10223384986918897No Hit
NNGGANNNNGNTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1260.10142885892533708No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1260.10142885892533708No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1260.10142885892533708No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACACAGCTGGGAGGC1250.1006238679814852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)