FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128043
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT6180.48265035964480685No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5350.4178283857766532No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT4710.3678451770108479No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3740.29208937622517434No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG3650.28506048749248303No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3600.28115554930765446No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT3600.28115554930765446No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3590.28037456167068875No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3560.27803159875979166No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3540.27646962348586024No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC3500.2733456729379974No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3430.26787875947923745No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC3330.2600688831095804No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3320.25928789547261466No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3070.239763204548472No Hit
NAGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT3030.23663925400060917No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2920.22804838999398638No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2880.2249244394461236No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2860.22336246417219216No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2850.22258147653522647No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2810.21945752598736362No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2770.2163335754395008No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2690.21008567434377515No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2610.20383777324804947No Hit
NNGGANNNNGNTATGNNNACACATACTCCCTTGCTCCAGGGCTGCAGAAC2540.1983708597892895No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC2520.1968088845153581No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2500.1952469092414267No Hit
NNGGANNNNGNTATGNNNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA2470.1929039463305296No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2470.1929039463305296No Hit
NNGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2420.18899900814570103No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC2420.18899900814570103No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2400.18743703287176966No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT2350.18353209468694112No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2290.17884616886514687No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2260.17650320595424973No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2260.17650320595424973No Hit
NNGGANNNNGNTATGNNNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC2240.17494123068031833No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG2190.1710362924954898No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2120.16556937903672986No Hit
NNGGANNNNGNTATGANNTCCTGCCCCACCCTGATACAGGTCACTGTGGA2110.16478839139976414No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2060.1608834532149356No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2030.15854049030403847No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2030.15854049030403847No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2020.15775950266707278No Hit
NAGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT2010.15697851503010707No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1990.15541653975617567No Hit
NNGGANNNNGNTATGNNNGGGCCTTCTCGAAGCTCTCCCAGGAAACAGCT1970.15385456448224424No Hit
NNGGANNNNGNTATGANNACACATACTCCCTTGCTCCAGGGCTGCAGAAC1940.15151160157134713No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1940.15151160157134713No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA1930.15073061393438142No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1900.1483876510234843No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1880.14682567574955288No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1870.14604468811258717No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1860.14526370047562145No Hit
NAGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1840.14370172520169006No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1830.14292073756472437No Hit
NNGGANNNNGNTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA1830.14292073756472437No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1810.14135876229079294No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1810.14135876229079294No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1790.13979678701686152No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1770.13823481174293012No Hit
NAGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1770.13823481174293012No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1760.1374538241059644No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1740.13589184883203298No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1740.13589184883203298No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1740.13589184883203298No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1730.1351108611950673No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1720.13432987355810158No Hit
NNGGANNNNGNTATGNNNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1720.13432987355810158No Hit
NNGGANNNNGNTATGNNNCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT1710.13354888592113587No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1690.13198691064720444No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1680.13120592301023873No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC1680.13120592301023873No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGCTCCTCCTCGC1640.1280819724623759No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1630.12730098482541022No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1600.1249580219145131No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1600.1249580219145131No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1580.12339604664058168No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA1570.12261505900361598No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1550.12105308372968455No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1530.11949110845575314No Hit
NNGGANNNNGNTATGANNAGGCAGTAACTTGGGCATTTCTTCTGATTCTC1520.11871012081878743No Hit
NAGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCAGACCTCGCTC1520.11871012081878743No Hit
NNGGANNNNGNTATGANNATCCACTGTTGGCCAACACACCTGGCCTTCAA1480.1155861702709246No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGACAGGAAACA1480.1155861702709246No Hit
NAGGANNNNGNTATGNNNTCCTGCCCCACCCTGATACAGGTCACTGTGGA1480.1155861702709246No Hit
NAGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1470.11480518263395889No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1440.1124622197230618No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1430.11168123208609608No Hit
NAGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1410.11011925681216467No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1400.10933826917519897No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1400.10933826917519897No Hit
NNGGANNNNGNTATGNNNGGGTCCCACCCATGCTGAAATGCGATCCTCAA1380.10777629390126754No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1380.10777629390126754No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1380.10777629390126754No Hit
NNGGANNNNGNTATGANNGGGCCTTCTCGAAGCTCTCCCAGGAAACAGCT1360.10621431862733613No Hit
NNGGANNNNGNTATGNNNGATGCTCCTTCCTTGGCCCCAGGCCCTGGCGT1350.10543333099037042No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGACCTCTCCTCCT1340.10465234335340472No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1340.10465234335340472No Hit
NAGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1330.103871355716439No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACGGAGATCCAGCC1330.103871355716439No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1330.103871355716439No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1320.1030903680794733No Hit
NNGGANNNNGNTATGANNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1310.10230938044250759No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1300.10152839280554188No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1300.10152839280554188No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCCGTCCTCGGAC1290.10074740516857618No Hit
NAGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1290.10074740516857618No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1290.10074740516857618No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)