FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78892
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT18292.3183592759722154No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT17102.167520154134767No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG13151.6668356740860923No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT11661.4779698828778582No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA10871.3778329868681236No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT10621.3461440957258024No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA10301.3055823150636314No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT9681.2269938650306749No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT9531.2079805303452822No Hit
CAGGAAANAGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT9281.176291639202961No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8771.1116463012726259No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT7690.9747502915377985No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA5920.7503929422501647No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5830.7389849414389291No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG5830.7389849414389291No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5800.7351822745018506No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT5050.6401156010748872No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4940.6261724889722659No Hit
CAGGAAANAGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT4730.5995538204127161No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4610.584343152664402No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4390.5564569284591594No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4340.5501191502306951No Hit
CAGGAAANAGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT4120.5222329260254525No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4110.5209653703797597No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT4090.5184302590883739No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3920.49688181311159557No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT3910.4956142574659027No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG3890.49307914617451704No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3880.49181159052882417No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT3760.4766009227805101No Hit
CAGGAAANAGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA3700.468995588906353No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT3670.4651929219692745No Hit
CAGGAAANNGCTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3650.4626578106778888No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3070.3891395832277037No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2850.3612533590224611No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG2730.34604269127414694No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2690.34097246869137554No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2660.33716980175429706No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2540.32195913400598286No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2180.27632713076104043No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG2120.2687217968868833No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1910.2421031283273336No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1910.2421031283273336No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1910.2421031283273336No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1830.2319626831617908No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA1800.22816001622471227No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1790.22689246057901943No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1770.22435734928763373No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1720.2180195710591695No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1720.2180195710591695No Hit
CAGGAAANNGCTATGANNCGTAAAGGGCAAGGTGGGGTAATCTTGTCCAT1720.2180195710591695No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1710.21675201541347666No Hit
CAGGAAACAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1700.21548445976778383No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1660.21041423718501243No Hit
CAGGAAANNGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1640.20787912589362673No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1640.20787912589362673No Hit
CAGGAAANNGCTATGANNGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT1620.20534401460224105No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1620.20534401460224105No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1600.20280890331085535No Hit
CAGGAAACNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1580.20027379201946965No Hit
CAGGAAANNGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT1570.19900623637377682No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1550.19647112508239112No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1500.19013334685392688No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1480.18759823556254118No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1470.18633067991684835No Hit
CAGGAAANNGCTATGANNACCCCACCTGCTCAAAAGACACACTCCTAAAA1460.1850631242711555No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1430.18126045733407697No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1410.17872534604269127No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1370.17365512345991987No Hit
CAGGAANNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1350.17112001216853417No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTCCTCCTCGCT1320.16731734523145567No Hit
CAGGAAANAGCTATGANNTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC1310.16604978958576283No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1280.1622471226486843No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1270.16097956700299143No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT1270.16097956700299143No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1250.15844445571160576No Hit
CAGGAAANAGCTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG1240.1571769000659129No Hit
CAGGAAANAGCTATGANNGGTCCCGCGGGGAAAGTAAGTCCTTCCCTTGG1240.1571769000659129No Hit
CAGGAAACNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1240.1571769000659129No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1230.15590934442022006No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1230.15590934442022006No Hit
CAGGAAACNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1220.1546417887745272No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1180.14957156619175582No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1160.14703645490037012No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA1160.14703645490037012No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1140.14450134360898442No Hit
CAGGAAACNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1130.1432337879632916No Hit
CAGGAAANAGCTATGANNTCCAGTGGTGGGGCTCAACAAAGGCTCTGTGA1120.14196623231759875No Hit
CAGGAAACAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT1120.14196623231759875No Hit
CAGGAAACAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT1100.13943112102621305No Hit
CAGGAAANAGCTATGANNTCCACAAGGCCCTGGGACAATCTCCCAGGCCC1100.13943112102621305No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1070.13562845408913451No Hit
CAGGAAACAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1040.13182578715205598No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1030.13055823150636311No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA990.12548800892359174No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT990.12548800892359174No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT990.12548800892359174No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG980.12422045327789889No Hit
CAGGAAACAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA980.12422045327789889No Hit
CAGGAAANAGCTATGANNTGGGTTATGGCGTGGGATGCCCTGCAACCCCC960.12168534198651319No Hit
NAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA960.12168534198651319No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG960.12168534198651319No Hit
CAGGAANNNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT940.11915023069512752No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA930.11788267504943468No Hit
NAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT900.11408000811235613No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT890.1128124524666633No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT890.1128124524666633No Hit
CAGGANANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT880.11154489682097045No Hit
CAGGAAACNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA860.10900978552958475No Hit
NAGGANANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT860.10900978552958475No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCACTGGGTCTCC860.10900978552958475No Hit
NAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG850.10774222988389191No Hit
CAGGAANNNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA840.10647467423819906No Hit
CAGGAAANAGCTATGANNTCTGACTGTCCGTGATCCCACACAATCACCCT840.10647467423819906No Hit
CAGGAANNNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG840.10647467423819906No Hit
NAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT830.10520711859250621No Hit
CAGGAAANAGCTATGANNTGTTGTCTTTCAGGGGCCGCAGCACAGGGACG830.10520711859250621No Hit
CAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA810.10267200730112053No Hit
CAGGAAANAGCTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC810.10267200730112053No Hit
CAGGAAACAGCTATGANCCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT810.10267200730112053No Hit
CAGGAAACAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT810.10267200730112053No Hit
NAGGANANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA800.10140445165542768No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT800.10140445165542768No Hit
CAGGAAACNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA790.10013689600973483No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC790.10013689600973483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)