FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81133
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA3850.47452947629201436No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3400.41906499205009057No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3180.3919490219762612No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC2820.34757743458272217No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT2750.33894962592286737No Hit
NNGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2750.33894962592286737No Hit
NNGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA2720.33525199364007247No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC2540.313066199943303No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2540.313066199943303No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2460.3032058471891832No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2360.29088040624653344No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT2270.2797875093981487No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT2250.27732242120961875No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2100.2588342597956442No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1970.24281118657019954No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1910.2354159220046097No Hit
NAGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA1850.2280206574390199No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1800.221857936967695No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1780.21939284877916507No Hit
NNGGANNNNGNTATGNNNTGCACACACCTCCTATGTTAACAGGCACAAGT1770.2181603046849001No Hit
NNGGANNNNGNTATGANNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA1760.21692776059063515No Hit
NNGGANNNNGNTATGANNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1750.21569521649637016No Hit
NNGGANNNNGNTATGANNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1690.20829995193078032No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1690.20829995193078032No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1660.20460231964798542No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1650.20336977555372046No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC1640.20213723145945545No Hit
NNGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1620.1996721432709255No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1620.1996721432709255No Hit
NNGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT1590.1959745109881306No Hit
NAGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1550.1910443346110707No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1520.18734670232827577No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1510.1861141582340108No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1510.1861141582340108No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1480.1824165259512159No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1480.1824165259512159No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1430.17625380547989103No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1420.17502126138562607No Hit
NNGGANNNNGNTATGANNTGGAGTTTGAAACAGCACCTTGGCACTCCTTC1350.16639345272577127No Hit
NAGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1310.16146327634871138No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1310.16146327634871138No Hit
NNGGANNNNGNTATGNNNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1310.16146327634871138No Hit
NAGGANNNNGNTATGNNNTCCACAAGGCCCTGGGACAAGGAAAATGGAGA1300.1602307322544464No Hit
NNGGANNNNGNTATGANNACCTTTACCCAAATAGCTACCACCAGTGCCTT1300.1602307322544464No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGACACCCAAGACA1290.1589981881601814No Hit
NAGGANNNNGNTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA1270.1565330999716515No Hit
NNGGANNNNGNTATGNNNTGGAGTTTGAAACAGCACCTTGGTGCTCCTTC1250.15406801178312154No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1240.15283546768885656No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC1240.15283546768885656No Hit
NNGGANNNNGNTATGNNNTCCCACTTGCTCTTTGAAGGCAGGCACTACGT1220.1503703795003266No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1210.14913783540606165No Hit
NNGGANNNNGNTATGNNNAGGTTGCTGGATTCAGTTTGCTGACCAACTTT1180.14544020312326672No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1180.14544020312326672No Hit
NNGGANNNNGNTATGANNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC1170.14420765902900176No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1150.14174257084047182No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC1140.14051002674620683No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1140.14051002674620683No Hit
NNGGANNNNGNTATGANNTGCACACACCTCCTATGTTAACAGGCACAAGT1130.13927748265194187No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA1130.13927748265194187No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1120.1380449385576769No Hit
NNGGANNNNGNTATGNNNGGGCATGGTGTGGGACTAGCTAATCTAGGGAC1110.13681239446341192No Hit
NNGGANNNNGNTATGNNNACCTGGCAGCCCCAAATCTGCGGGTGACCACG1110.13681239446341192No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1100.13557985036914694No Hit
NNGGANNNNGNTATGNNNCTCACCAAGAGTGCCCCACGCCCAAGACACTG1090.13434730627488198No Hit
NAGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1080.13311476218061702No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1060.13064967399208707No Hit
NAGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1040.12818458580355713No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1030.12695204170929214No Hit
NNGGANNNNGNTATGANNCTGGTCCTCATCCTTGGGGCCGGAGAGCCTGT1020.12571949761502718No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1020.12571949761502718No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1020.12571949761502718No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA1020.12571949761502718No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1010.12448695352076222No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1010.12448695352076222No Hit
NNGGANNNNGNTATGANNCTCACCAAGAGTGCCCCACGACACCCAAGACA1000.12325440942649722No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGTCGTTTTACATG990.12202186533223226No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCCGTCGTTTTAC980.12078932123796729No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGACACTGCCCACC970.11955677714370232No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG970.11955677714370232No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG970.11955677714370232No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA940.1158591448609074No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT940.1158591448609074No Hit
NAGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT930.11462660076664243No Hit
NNGGANNNNGNTATGNNNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA930.11462660076664243No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT920.11339405667237747No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC890.10969642438958253No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC890.10969642438958253No Hit
NAGGANNNNGNTATGNNNATTAGCCAGGTGTGGTGGCGTGCACCTCCTTC890.10969642438958253No Hit
NAGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT880.10846388029531757No Hit
NAGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC880.10846388029531757No Hit
NAGGANNNNGNTATGNNNACCTTTACCCAAATAGCTACCACCAGTGCCTT880.10846388029531757No Hit
NNGGANNNNGNTATGANNGGGCATGGTGTGGGACTAGCTAATCTAGGGAC870.1072313362010526No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG870.1072313362010526No Hit
NNGGANNNNGNTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC840.10353370391825768No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC840.10353370391825768No Hit
NNGGANNNNGNTATGANNACCTGGCAGCCCCAAATCTGCGGGTGACCACG830.10230115982399271No Hit
NNGGANNNNGNTATGNNNAGGCTTTGTGTGTGGGGACTGCAGTGAACTGA820.10106861572972772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)