FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142931
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG42402.966466336903821No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG38862.7187943833038317No Hit
TGTAAAANGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGTATGTGTG19581.3698917659570002No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT18391.2866348098033318No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGTATGTGTG18101.2663452994801687No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG17831.247455065731017No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA17221.2047771302236743No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT17171.2012789387886462No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG17041.1921836410575732No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT16911.1830883433265003No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA16841.1781908753174608No Hit
TGTAAAANGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA16221.1348133015231125No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG16031.1215201740700058No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA15821.1068277700428877No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC15791.104728855181871No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG15521.0858386214327194No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT15331.0725454939796126No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC14361.0046805801400676No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG12900.9025333902372473No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG12110.8472619655638036No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT12070.844463412415781No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT11930.8346684763977024No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT11860.829771008388663No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA11660.8157782426485507No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT11460.8017854769084383No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA11170.7814959665852754No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT11020.7710013922801912No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT10480.7332209247818877No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA10440.7304223716338653No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA10200.7136310527457305No Hit
TGTAAAANGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT9710.6793487766824552No Hit
TGTAAAACGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT9050.6331726497400844No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC9040.6324730114530788No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC9030.6317733731660731No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT8310.5813994165016686No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC7920.5541135233084495No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT7740.5415200341423484No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG7700.5387214809943259No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT7630.5338240129852867No Hit
TGTAAAANGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG7440.5205308855321799No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7400.5177323323841574No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC7130.49884209863500567No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG7080.4953439071999776No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTCCGGTCATAGCT7070.494644268912972No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG6970.48764788604291587No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT6830.47785295002483713No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6710.46945729058076974No Hit
TGTAAAANGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT6670.4666587374327473No Hit
TGTAAAANGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG6420.44916778025760684No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6150.4302775465084552No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC6100.42677935507342707No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA6100.42677935507342707No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC6080.4253800784994158No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT5980.4183836956293596No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA5870.4106876744722978No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC5480.3834017812790787No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC5470.38270214299207306No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG5380.37640539840902254No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA5300.3708082921129776No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT5290.37010865382597197No Hit
TGTAAAANGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA5290.37010865382597197No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT5270.36870937725196073No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT5250.3673101006779495No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT5240.36661046239094386No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT5200.3638119092429214No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT4980.34841986692879784No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT4940.3456213137807754No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT4820.3372256543367079No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4710.3295296331796461No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC4710.3295296331796461No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC4670.32673108003162366No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA4660.326031441744618No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG4630.3239325268836012No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC4580.3204343354485731No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT4540.3176357823005506No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG4480.3134379525785169No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT4480.3134379525785169No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT4450.31133903771750004No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG4440.3106393994304944No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG4340.30364301656043824No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA4320.30224373998642706No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4280.29944518683840454No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT4150.2903498891073315No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT4020.28125459137625847No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3890.27215929364518543No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTGCCTTTGGTCAT3860.27006037878416855No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTGCCTTTGGTCAT3820.2672618256361461No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTAGCTATTTGGGTAAAGGTG3430.239975932442927No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTAGCTATTTGGGTAAAGGTG3380.23647774100789892No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC3380.23647774100789892No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT3350.23437882614688205No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC3320.2322799112858652No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3320.2322799112858652No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA3300.23088063471185397No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG3250.22738244327682589No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG3230.22598316670281465No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA3210.22458389012880342No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT3180.22248497526778654No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3170.22178533698078096No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT3150.22038606040676972No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3060.21408931582371912No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2890.20219546494462362No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2870.2007961883706124No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2870.2007961883706124No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2820.1972979969355843No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC2770.19379980550055623No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2660.18610378434349442No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT2510.17560921003841012No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGTCATAGCTGTT2430.1700121037423652No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2400.16791318888134835No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT2370.1658142740203315No Hit
TGTAAAANGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG2360.16511463573332588No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT2330.163015720872309No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC2320.1623160825853034No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2310.16161644429829777No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGAGGCTGTCTG2280.15951752943728092No Hit
TGTAAAANGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGTCATAGCTGTT2160.15112186999321353No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2060.14412548712315734No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG2060.14412548712315734No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG2010.14062729568812923No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1960.13712910425310115No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC1950.13642946596609554No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTCATTTCAGGGGCAAAA1940.13572982767908992No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1930.13503018939208428No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGTCTGCCGGTCA1900.13293127453106743No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTGAGGGTCATAGC1890.1322316362440618No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1840.12873344480903373No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGAGGCTGTCTG1830.12803380652202812No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCTGTCTGCCGGTCA1820.1273341682350225No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTCATTTCAGGGGCAAAA1770.1238359767999944No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1770.1238359767999944No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTGAGGGTCATAGC1740.12173706193897756No Hit
TGTAAAANGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCGGTCATAGCTG1600.11194212592089889No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACCGCAACC1560.10914357277287641No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTTACTGCAACC1530.10704465791185956No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1470.10284682818982586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)