FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142931
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT28501.9939691179660115No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT22521.5755854223366519No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT17251.2068760450846912No Hit
CAGGAAANAGCTATGANNACACATACTCCCTTGCTAACCTCTCTCTTCAA15451.0809411534236801No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT14351.003980941853062No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA12540.8773464119050451No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC12460.8717493056090002No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT11560.8087818597784945No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT10680.7472136905220002No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT10280.7192281590417754No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9760.6828469681174832No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9210.6443668623321743No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG8820.6170809691389552No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT8650.6051871182598596No Hit
CAGGAAANAGCTATGACCACACATACTCCCTTGCTAACCTCTCTCTTCAA7900.5527142467344384No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA7740.5415200341423484No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT7660.5359229278463035No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG6970.48764788604291587No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC6900.48275041803387647No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT6860.479951864885854No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC6810.47645367345082595No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC6730.470856567154781No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA6600.46176126942370793No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6320.4421713973875506No Hit
CAGGAAANAGCTATGANNTCCTTCCACCACCAGACAACTGGCCGTCGTTT6110.4274789933604327No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5840.408588759611281No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTAACCTCTCTCTTCAA5570.3896985258621293No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG5470.38270214299207306No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC5430.3799035898440506No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT5400.3778046749830338No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC5210.36451154752992704No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT5140.3596140795208877No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC5120.35821480294687646No Hit
CAGGAAANAGCTATGANCACACATACTCCCTTGCTAACCTCTCTCTTCAA5060.3540169732248427No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG5060.3540169732248427No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA4840.33862493091071916No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4630.3239325268836012No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC4620.32323288859659555No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4590.3211339737355787No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC4520.3162365057265394No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC4480.3134379525785169No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA4450.31133903771750004No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT4440.3106393994304944No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC4410.3085404845694776No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4220.29524735711637085No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4180.2924488039683484No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG4160.29104952739433715No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3970.2777563999412304No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA3960.27705676165422477No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT3950.2763571233672192No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3930.27495784679320795No Hit
CAGGAAANAGCTATGACCTCCTTCCACCACCAGACAACTGGCCGTCGTTT3910.2735585702191967No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC3910.2735585702191967No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3860.27006037878416855No Hit
CAGGAAANAGCTATGANNCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT3780.2644632724881236No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3760.2630639959141124No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC3710.25956580447908434No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3660.25606761304405623No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3620.25326905989603377No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3570.24977086846100566No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3550.24837159188699443No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG3440.24067557072993262No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3390.2371773792949045No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3380.23647774100789892No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG3380.23647774100789892No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG3360.2350784644338877No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3290.23018099642484838No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA3220.225283528415809No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT3220.225283528415809No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3140.21968642211976408No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT3100.2168878689717416No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC3060.21408931582371912No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2980.20849220952767422No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2970.20779257124066858No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG2940.20569365637965173No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGAGTCCCTGGGAACTGACTGGC2880.20149582665761803No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2840.19869727350959554No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2820.1972979969355843No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2780.19449944378756184No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA2780.19449944378756184No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2770.19379980550055623No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA2740.19170089063953935No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC2740.19170089063953935No Hit
CAGGAAANAGCTATGANCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2700.1889023374915169No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2610.1826055929084663No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2600.1819059546214607No Hit
CAGGAAANAGCTATGANNACCTTTACCCAAATAGCTACCACCACGTACCA2570.17980703976044385No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC2550.17840776318643262No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC2480.1735102951773933No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2470.1728106568903877No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2470.1728106568903877No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2460.17211101860338207No Hit
CAGGAAACAGCTATGACCTCCTTCCACCACCAGACAACTGGCCGTCGTTT2460.17211101860338207No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2450.17141138031637643No Hit
CAGGAAANAGCTATGANCTCCTTCCACCACCAGACAACTGGCCGTCGTTT2450.17141138031637643No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2450.17141138031637643No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2440.1707117420293708No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2430.1700121037423652No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2350.16441499744632027No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2330.163015720872309No Hit
CAGGAAANAGCTATGANNGCAGCTCCCCACAACTTCAACTGGCCGTCGTT2320.1623160825853034No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTGGCCGTC2310.16161644429829777No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2290.16021716772428654No Hit
CAGGAAANAGCTATGACCCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT2230.15601933800225282No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC2220.1553196997152472No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2140.14972259341920227No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA2140.14972259341920227No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2130.14902295513219666No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA2100.1469240402711798No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2100.1469240402711798No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC2090.1462244019841742No Hit
CAGGAAANAGCTATGANNTGGCATCCAGGGCCTATTTGGTTCCTATTGAG2080.14552476369716857No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2080.14552476369716857No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2070.14482512541016296No Hit
CAGGAAANAGCTATGANNACAGGGGCCTCTTAAAACTGCATGTGTTCAGC2060.14412548712315734No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2060.14412548712315734No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2060.14412548712315734No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2040.14272621054914608No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2010.14062729568812923No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1990.139228019114118No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1980.13852838082711239No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1980.13852838082711239No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1970.13782874254010677No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1920.13433055110507866No Hit
CAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1910.13363091281807304No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1910.13363091281807304No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1900.13293127453106743No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1890.1322316362440618No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1890.1322316362440618No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1880.1315319979570562No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGAGTCCCTGGGAACTGACTGGC1860.13013272138304496No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1830.12803380652202812No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1800.12593489166101127No Hit
CAGGAAANAGCTATGANNCCACCCTCATGCCTAACCCCTATTTTCCAAAG1800.12593489166101127No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1780.12453561508700003No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1760.12313633851298879No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1760.12313633851298879No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTCCACCACCAGA1760.12313633851298879No Hit
CAGGAAANAGCTATGANNACAGGCAGGAGGGAGTACTACTGGCCGTCGTT1760.12313633851298879No Hit
CAGGAAANAGCTATGANCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1760.12313633851298879No Hit
CAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1740.12173706193897756No Hit
CAGGAAANAGCTATGANNACCCCTTAAGCCAGAGCCGACTGGCCGTCGTT1730.12103742365197194No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1720.12033778536496631No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1700.11893850879095508No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1670.11683959392993823No Hit
CAGGAAANAGCTATGANNGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1670.11683959392993823No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1670.11683959392993823No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1640.11474067906892137No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1640.11474067906892137No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1640.11474067906892137No Hit
CAGGAAANAGCTATGANNTGACTGCAAACATAGAAGTGAGCAGAGCAAGA1630.11404104078191575No Hit
CAGGAAACAGCTATGACCCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1630.11404104078191575No Hit
NAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1630.11404104078191575No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1620.11334140249491013No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1580.11054284934688766No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1580.11054284934688766No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCACGGTGGCTCAT1560.10914357277287641No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1560.10914357277287641No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATACAGAACCTTG1540.10774429619886518No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1540.10774429619886518No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1460.10214718990282025No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1460.10214718990282025No Hit
CAGGAAANAGCTATGANNGCAGCCACCCTGAACACACTTGGCTATCACCC1440.10074791332880902No Hit
CAGGAAANAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1430.1000482750418034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)