FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139646
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG36142.5879724446099424No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG33762.417541497787262No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG26951.929879839021526No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG26711.9126935250562134No Hit
TGTAAAANGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC22351.6004754880197072No Hit
TGTAAAANGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT20151.442934276671011No Hit
TGTAAAACGACGGCCAGTTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGC19891.424315769875256No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT18011.2896896438136431No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT17641.2631940764504532No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT15091.0805894905690103No Hit
TGTAAAANGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT14341.0268822594274094No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA13870.993225727912006No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC13840.9910774386663421No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCATATAGAA13490.9660140641335949No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC13250.9488277501682827No Hit
TGTAAAACGACGGCCAGTGGAGCGACTTGACAATGCCCCTCACACCCCTT12880.922332182805093No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC12740.912306832991994No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC12710.9101585437463301No Hit
TGTAAAANGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG12470.8929722297810176No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC12430.8901078441201323No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT11510.8242269739197685No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC11260.806324563539235No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGGAGCCAGTGAAAACGGTCAT11250.8056084671240136No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGCCTCACAGGGTCATAGCTG11150.7984475029718002No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGGAGCCAGTGAAAACGGTCAT11050.7912865388195865No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG10950.7841255746673732No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGCCTCACAGGGTCATAGCTG10480.7504690431519699No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTGAAAACGGTCAT10450.7483207539063059No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTGAAAACGGTCAT10320.7390115005084285No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT10170.7282700542801083No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC10130.7254056686192228No Hit
TGTAAAANGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC9980.7146642223909028No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC9660.6917491371038196No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC9360.6702662446471792No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC9230.6609569912493017No Hit
TGTAAAANGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT9060.648783352190539No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC9020.6459189665296535No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8950.6409062916231042No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC8780.6287326525643413No Hit
TGTAAAANGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC8720.6244360740730132No Hit
TGTAAAANGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT8680.6215716884121278No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC8510.609398049353365No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA8220.588631253311946No Hit
TGTAAAANGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA7530.5392206006616731No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT7460.5342079257551237No Hit
TGTAAAANGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT7370.5277630580181315No Hit
TGTAAAACGACGGCCAGTCCCTGTCCTGAGCACCGTTGCTAACCCCCAGT7300.5227503831115822No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC7210.51630551537459No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA7170.5134411297137047No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA6800.48694556235051484No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT6720.4812167910287441No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC6520.46689486272431724No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG6410.4590178021568824No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG6370.456153416495997No Hit
TGTAAAANGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT6350.45472122366555434No Hit
TGTAAAANGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG6210.44469587385245546No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG5760.41247153516749496No Hit
TGTAAAANGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT5640.4038783781848388No Hit
TGTAAAACGACGGCCAGTACACTCCCTCCACCTCCCATCCCAGAAAAAGT5630.4031622817696175No Hit
TGTAAAANGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC5460.3909886427108546No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCGGTCATAGCT5290.3788150036520917No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG5110.3659252681781075No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5110.3659252681781075No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGCGCTCATATAGAAG5080.3637769789324435No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCGGTCATAGCT5000.3580482076106727No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG4950.35446772553456596No Hit
TGTAAAANGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT4940.3537516291193446No Hit
TGTAAAANGACGGCCAGTCGGTGCTCTGGTGAGATGCGCTCATATAGAAG4920.35231943628890194No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC4890.35017114704323793No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG4730.3387136043996964No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG4670.3344170259083683No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT4650.33298483307792565No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4550.32582386892571213No Hit
TGTAAAACGACGGCCAGTACACATTCTTGTAAAGCATGGGATTGCTGAAT4520.3236755796800481No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC4340.3107858442060639No Hit
TGTAAAANGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC4200.3007604943929651No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA4190.30004439797774374No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC3990.2857224696733168No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC3730.26710396287756183No Hit
TGTAAAANGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3590.257078613064463No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA3580.25636251664924165No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC3570.25564642023402034No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC3520.25206593815791356No Hit
TGTAAAACGACGGCCAGTGTGTTCAGGGTGGCTGCGGTCATAGCTGTTTC3460.24776935966658553No Hit
TGTAAAACGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA3380.24204058834481476No Hit
TGTAAAANGACGGCCAGTGTGTTCAGGGTGGCTGCGGTCATAGCTGTTTC3360.24060839551437205No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA3290.23559572060782263No Hit
TGTAAAANGACGGCCAGTGGTGTTACCGTGGTCACCCGCAGGGACACGGA3280.23487962419260128No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC3270.23416352777737995No Hit
TGTAAAANGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT3100.22198988871861705No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2830.20265528550764073No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG2800.20050699626197674No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT2700.19334603210976326No Hit
TGTAAAANGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG2700.19334603210976326No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT2680.19191383927932057No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2670.19119774286409924No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2550.18260458588144307No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA2530.18117239305100039No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2500.17902410380533634No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA2400.17186313965312291No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT2380.17043094682268023No Hit
TGTAAAANGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA2130.1525285364421466No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2130.1525285364421466No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2110.15109634361170388No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1980.1417870902138264No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1980.1417870902138264No Hit
TGTAAAANGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1970.14107099379860505No Hit
TGTAAAANGACGGCCAGTCATAGCCCAGGTCCAGCAGCCATCAGGTTCCC1950.13963880096816234No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1940.138922704552941No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC1900.13605831889205564No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1890.13534222247683428No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1840.13176174040072758No Hit
TGTAAAANGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1830.13104564398550622No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1820.13032954757028486No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT1770.12674906549417814No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC1700.12173639058762871No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC1690.12102029417240738No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC1650.118155908511522No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA1640.11743981209630064No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT1610.11529152285063662No Hit
TGTAAAACGACGGCCAGTAACAAATTCAGGTGAGATGCGCTCACATAGAA1540.1102788479440872No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1540.1102788479440872No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1520.1088466551136445No Hit
TGTAAAANGACGGCCAGTCCACCTGCCAGTGTCTTGGGCATTTCCTTGGC1520.1088466551136445No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1490.10669836586798045No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1480.10598226945275913No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT1400.10025349813098837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)