FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233738
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG57212.4476122838391703No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC56152.4022623621319594No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC45321.9389230677082887No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC37211.5919533837031203No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC32991.411409355774414No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA31591.3515132327648904No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG29261.2518289708990409No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT28291.2103295142424424No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC27801.189365871189109No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT22550.964755409903396No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA21810.9330960305983623No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC20080.8590815357365938No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG19780.8462466522345532No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA18580.7949071182263903No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA18040.7718043279227169No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA17350.7422840958680231No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT16700.7144751816136016No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC16400.7016402981115608No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG15050.6438833223523774No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC14460.618641384798364No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT14010.5993890595453029No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG14000.5989612300952348No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG13750.5882654938435342No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT12320.5270858824838066No Hit
CAGGAAANAGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT12150.5198127818326502No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC11850.5069778983306095No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG11090.4744628601254396No Hit
CAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA10930.4676175889243512No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG10720.45863317047292274No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC10090.4316799151186371No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC10050.429968597318365No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG10020.428685108968161No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA9170.3923196057123788No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT8900.3807682105605421No Hit
CAGGAAANAGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG8090.34611402510503214No Hit
CAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT7790.3332791416029914No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT7340.31402681634993024No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA7280.3114598396495221No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA6470.2768056541940121No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA6410.274238677493604No Hit
CAGGAAANAGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC6410.274238677493604No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6350.2716717007931958No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6200.2652542590421754No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6100.2609759645414952No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC5920.2532750344402707No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC5910.2528472049902027No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG5750.24600193378911428No Hit
CAGGAAANNGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5750.24600193378911428No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG5690.24343495708870616No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA5320.22760526743618925No Hit
CAGGAAANNGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT5280.22589394963591714No Hit
CAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG4990.21348689558394443No Hit
CAGGAAANNGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG4920.21049208943346825No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT4870.2083529421831281No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4820.20621379493278802No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGTGCCT4500.1925232525306112No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4450.19038410528027105No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4420.189100616930067No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4380.1873892991297949No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA4370.18696146967972688No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4260.1822553457289786No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4220.1805440279287065No Hit
CAGGAAANNGCTATGANNCCTTGGGCAAGTTAACAAACCTCTCTGAGCCT4130.17669356287809426No Hit
CAGGAAANAGCTATGANNAGGACCACCCCTCCATCCTGGTGCCTGGCTTG4100.1754100745278902No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3970.16984829167700588No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3960.16942046222693785No Hit
CAGGAAANNGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3860.16514216772625762No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3850.1647143382761896No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3820.16343084992598553No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3750.16043604377550932No Hit
CAGGANANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3720.15915255542530526No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG3670.15701340817496512No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3590.15359077257442094No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3560.15230728422421685No Hit
CAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3520.15059596642394477No Hit
NAGGANANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3520.15059596642394477No Hit
CAGGAAANNGCTATGANNAACACGGCAGTCAGAGGTGCAGGAAACAGCTA3480.14888464862367265No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3440.14717333082340056No Hit
CAGGAAANNGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC3410.14588984247319647No Hit
NAGGANANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3380.14460635412299241No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCGG3380.14460635412299241No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAATG3330.1424672068726523No Hit
NAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC3290.1407558890723802No Hit
NAGGAAANNGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3280.14032805962231218No Hit
NAGGAAANNGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3270.13990023017224415No Hit
NAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3260.13947240072217612No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3250.1390445712721081No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3210.137333253471836No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3180.13604976512163192No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3130.13391061787129177No Hit
CAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG3100.1326271295210877No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3000.12834883502040748No Hit
CAGGAAANAGCTATGANNAGGTCCAGATGGCTGGTGACTACCTCAGGGTG2980.12749317612027142No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2940.12578185831999933No Hit
NAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2930.1253540288699313No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2920.12492619941986327No Hit
CAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2910.12449836996979524No Hit
CAGGAAANNGCTATGANNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG2900.12407054051972721No Hit
CAGGAAANAGCTATGANNAGAGCTACTTCTGTGCCCACCATATTCCTCTA2860.12235922271945511No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2840.12150356381931907No Hit
CAGGAAACAGCNATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2830.12107573436925105No Hit
CAGGAAANAGCTATGANNAACACCCTTGGCCTCCCTCACTGGCCGTCGTT2830.12107573436925105No Hit
CAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2830.12107573436925105No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2810.12022007546911499No Hit
NAGGANANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2790.11936441656897893No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2790.11936441656897893No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2730.11679743986857079No Hit
NAGGANANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2680.11465829261823068No Hit
NAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2670.11423046316816265No Hit
CAGGAAANNGCTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC2620.11209131591782251No Hit
NAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2610.11166348646775448No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2540.10866868031727832No Hit
NAGGAAANNGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2500.10695736251700623No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG2490.1065295330669382No Hit
NAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2390.10225123856625795No Hit
CAGGAAACNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2350.10053992076598584No Hit
NAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2340.10011209131591782No Hit
CAGGAAANAGCTATGANNACCTGGCAGCCCCAAATCTGCACCTCCCGCAG2340.10011209131591782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)