FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109574
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG21841.9931735630715315No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT19561.7850950042893388No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT17401.5879679486009455No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT13831.2621607315604066No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT13631.243908226404074No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA13101.1955390877397922No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC12241.1170533155675617No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC11871.083286181028346No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT11051.0084509098873822No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9360.8542172413163707No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC9030.8241006078084216No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8950.8167996057458887No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT8510.7766440944019567No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT8380.7647799660503404No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG7280.6643911876905105No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG7110.6488765583076277No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6820.6224104258309453No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6340.5786044134557469No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT6300.5749539124244802No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT5980.545749904174348No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT5770.5265847737601986No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA5750.5247595232445653No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5570.5083322686038658No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT5550.5065070180882326No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC5090.4645262562286674No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4950.4517495026192345No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT4740.43258437220508517No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4660.425283370142552No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT4480.4088561155018526No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4310.3933414861189698No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT4260.3887783598298866No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC4220.3851278587986201No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC4200.38330260828298685No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3980.3632248526110209No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3710.3385839706499717No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3530.32215671600927226No Hit
CAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3300.30116633507948964No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3140.2865643309544235No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3120.2847390804387902No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA3090.2820012046653403No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC2970.2710497015715407No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2920.2664865752824575No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2830.25827294796210776No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2780.25370982167302464No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2690.24549619435267492No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGAGCAGTGGGG2680.24458356909485826No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2630.2400204428057751No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2560.23363206600105862No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2550.23271944074324202No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT2420.22085531239162576No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2420.22085531239162576No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG2390.21811743661817587No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2360.21537956084472593No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2290.2089911840400095No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT2230.20351543249310966No Hit
CAGGAAANNGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC2170.19803968094620986No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2140.19530180517275997No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2130.1943891799149433No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2070.1889134283680435No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2060.18800080311022688No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2060.18800080311022688No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2040.1861755525945936No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC1990.18161242630551044No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1960.17887455053206053No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1960.17887455053206053No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGCAGCC1930.1761366747586106No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1930.1761366747586106No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1880.17157354846952744No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1850.16883567269607755No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1830.16701042218044426No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1810.165185171664811No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1810.165185171664811No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1780.1624472958913611No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1780.1624472958913611No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1770.16153467063354446No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1760.16062204537572783No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1740.15879679486009454No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1710.15605891908664463No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGTACC1600.14602004125066165No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1520.13871903918812856No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1520.13871903918812856No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1510.13780641393031196No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1490.13598116341467867No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1460.13324328764122875No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1440.1314180371255955No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT1390.12685491083651232No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1360.12411703506306242No Hit
CAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1350.12320440980524577No Hit
CAGGAAANAGCTATGANNTGCTTCAGTCGCCTGCACAACAGGAAACAGCA1350.12320440980524577No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1350.12320440980524577No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1340.12229178454742913No Hit
CAGGAANNNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1330.12137915928961251No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1330.12137915928961251No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1330.12137915928961251No Hit
NAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1300.1186412835161626No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1290.11772865825834596No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1290.11772865825834596No Hit
NAGGANANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1270.1159034077427127No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1270.1159034077427127No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCCAGGAAACAGCT1270.1159034077427127No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1260.11499078248489605No Hit
NAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1260.11499078248489605No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1230.11225290671144615No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA1220.11134028145362951No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1220.11134028145362951No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1200.10951503093799625No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1200.10951503093799625No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT1200.10951503093799625No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1190.1086024056801796No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1180.10768978042236296No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1170.10677715516454633No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1160.10586452990672969No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1160.10586452990672969No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1150.10495190464891305No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1140.10403927939109643No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1140.10403927939109643No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)