FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119233
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA31392.6326604211921194No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG27592.313956706616457No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC23892.0036399318980482No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG22581.893771019768017No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA21711.820804643009905No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA19631.646356293979016No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT19321.6203567804215275No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT19301.6186793924500769No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG17761.4895205186483609No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG15661.3133947816460207No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA15351.2873952680885326No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCAGCACCTCT15141.2697826943882986No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG14801.241267098873634No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC14511.2169449732875965No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT13941.1691394161012472No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG13721.1506881484152878No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA13061.0953343453574094No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT13061.0953343453574094No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG12921.0835926295572533No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG12631.059270503971216No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGGGGTTAGGCAT11780.9879815151845547No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA11570.9703689414843206No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC11510.9653367775699682No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATAATGACACTGTG11290.9468855098840085No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC10750.9015960346548354No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG10260.8605000293542895No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT10050.8428874556540554No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG9810.8227587999966451No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC9770.8194040240537435No Hit
TGTAAAACGACGGCCAGTTGAGAAGCTGAGGCTTAAAGAGGGATCCCTTG9280.7783080187531976No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG9280.7783080187531976No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC9060.759856751067238No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC8040.6743099645232443No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG8010.6717938825660681No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT7850.6583747787944613No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG7810.6550200028515595No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC7610.638246123137051No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC7460.6256657133511695No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT7020.5887631779792507No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA6660.5585701944931353No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT6100.5116033312925112No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT5990.5023776974495315No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCACTTGAAAGGA5880.4931520636065519No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACAGGAC5740.4814103478063959No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA5490.4604429981632602No Hit
TGTAAAACGACGGCCAGTACTAAACTGTAGTGAACAGAGACATGCCCAGG5490.4604429981632602No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTAACTCATAGG5410.45373344627745676No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT5400.4528947522917313No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC5390.4520560583060059No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGGCCCCAAGGA5360.4495399763488296No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCGGCGGCTCCT5320.44618520040592785No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT5190.43528217859149737No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC5100.42773393271996846No Hit
TGTAAAACGACGGCCAGTAAGGGTGGGTCATGGGGTGAGGGTGGGTCGCG5040.42270176880561594No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAATTTTGG5030.4218630748198905No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTCTTCACTGTTG4980.4176696048912633No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGGCGGCACCTCT4930.41347613496263613No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC4840.40592788909110733No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT4750.39837964321957847No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGGCCCCAAGGA4700.3941861732909513No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGATACCAAGGT4620.38747662140514794No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT4530.379928375533619No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT4510.3782509875621682No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC4480.3757349056049919No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCAGCACGAC4400.3690253537191885No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4390.36818665973346304No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC4340.3639931898048359No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT4320.3623158018333851No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4290.3597997198762088No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG4270.35812233190475795No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAAGGAGAGT4220.35392886197613077No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4170.34973539204750365No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGCTTGACACGG4090.34302584016170024No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGGACTCCAG4050.3396710642187985No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG3900.32709065443291707No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGAGAGCGGTTCC3670.3078006927612322No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC3660.3069619987755068No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCAGCCCCAAGGAT3640.30528461080405594No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGCCAGTACCGGG3600.3019298348611542No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGACAAGCACCC3550.2977363649325271No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGGGGTGCCTCAA3510.29438158898962535No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG3500.2935428950038999No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGACGTGAG3350.28096248521801853No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAGGAGAGTGA3260.2734142393464896No Hit
TGTAAAACGACGGCCAGTTGCTGCATATTATTCCATTATGCAGATAGGAC3210.2692207694178625No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC3170.26586599347496076No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3110.2608338295606082No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC3100.2599951355748828No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGTTTGTGCAGGCG3100.2599951355748828No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG3020.2532855836890794No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3000.2516081957176285No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC2950.24741472578900134No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCACGGTCACTG2940.24657603180327595No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2910.24405994984609963No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGGCAGGGCAGCGT2860.2398664799174725No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGCCAGTTAACCA2770.23231823404594368No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC2730.22896345810304194No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCTGTGGAGATGG2710.2272860701315911No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGTGGTGCGATG2700.22644737614586566No Hit
TGTAAAACGACGGCCAGTGCCACTTCTGAAGGAGGTTCCCCCAGTAGTGC2600.21806043628861135No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT2520.21135088440280797No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG2510.21051219041708252No Hit
TGTAAAACGACGGCCAGTTTGCTGCTGGGCTTCCTCTGCCCTGAGGGCTT2510.21051219041708252No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAATTAGTAGT2470.2071574144741808No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGCTCCAGGATGGC2450.20548002650272995No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGACAGGGTCAGT2440.20464133251700453No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT2400.20128655657410283No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGCACAGGTGGACA2380.19960916860265196No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCACCAACAGGCCCC2370.1987704746169265No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGGATGCTGAGTGGGAG2290.1920609227311231No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCTGGAGACCCAGCGCG2240.18786745280249595No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGGAGGACCC2190.18367398287386882No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACATCTCCTGA2170.18199659490241796No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATCACGGTGGT2150.1803192069309671No Hit
TGTAAAACGACGGCCAGTTGTGGACCACAGTTTGCAAGTGTAAAACGACG2140.17948051294524167No Hit
TGTAAAACGACGGCCAGTAGCAGGCAGGTCCTTCCAGACAGCCAATGAGG2130.17864181895951625No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGGTCCCCGGATGCC2010.16857749113081108No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC2010.16857749113081108No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGACCTTGGTAGGAT1980.16606140917363482No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1970.1652227151879094No Hit
TGTAAAACGACGGCCAGTTCCTTCTTGGGTGGGTATTTTGCAACTGGGTA1930.16186793924500767No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCCTGCCA1920.16102924525928225No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGCAGGAAGCACCT1900.15935185728783138No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC1880.15767446931638054No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTACATGG1880.15767446931638054No Hit
TGTAAAACGACGGCCAGTGCCCTGAACCGGTGCCAAATGGAGTGAAGAAA1850.15515838735920426No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGGAGGTACAGGG1820.15264230540202797No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGGGAGGCGCCAG1790.1501262234448517No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1730.14509405953049911No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCAGCCCCTGCCA1710.14341667155904825No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGAAAGCCTCAGGCA1680.14090058960187196No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATTTCAGTCCACT1680.14090058960187196No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCCACGCTGGGAAT1650.13838450764469568No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGGTGCTCTGGT1650.13838450764469568No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG1610.13502973170179397No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACACCAGCCCC1610.13502973170179397No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAAAGGGGC1600.13419103771606855No Hit
TGTAAAACGACGGCCAGTCTCTCACCCACCTCCACCCATCAAAGGTGCCT1600.13419103771606855No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCGCCTCCTGCTCAGCC1600.13419103771606855No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTGTGAGGAGAATG1570.13167495575889226No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTGATAAGGAAGGA1560.13083626177316682No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAA1540.12915887380171598No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCATAGCTGTTTC1510.1266427918445397No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTATGCTGCGGCC1510.1266427918445397No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGGCCGCCCACAAT1490.12496540387308883No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACTGGCCCCTG1480.1241267098873634No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1480.1241267098873634No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGTCCCTGTGCTCGG1480.1241267098873634No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC1460.12244932191591254No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACAGGTGTGGCCT1410.1182558519872854No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCTGCCAA1350.11322368807293283No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCACCCCACCCCT1330.11154630010148196No Hit
TGTAAAACGACGGCCAGTCCACCTGCTTCGCACCATCTCGGCCCAGCCCA1320.11070760611575653No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCCGCCCCAAGGAT1310.10986891213003111No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT1310.10986891213003111No Hit
TGTAAAACGACGGCCAGTAGCTCCTATGGCCTGGGGAGTGGTTCCCACCA1310.10986891213003111No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA1280.10735283017285482No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACTGCCTTCCAGCATTT1270.10651413618712939No Hit
TGTAAAACGACGGCCAGTACCGGGCCAGGTCAGTTACTGTACCGGGGCCA1270.10651413618712939No Hit
TGTAAAACGACGGCCAGTCTGGGTTGGGGTGTCTGCTGGATGAAACCTCA1260.10567544220140399No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)