FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149831
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG40422.6977060821859298No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG38092.542197542564623No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA26161.7459671229585334No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG25001.668546562460372No Hit
TGTAAAANGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG24671.646521747835895No Hit
TGTAAAANGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT24461.6325059567112281No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT22431.4970199758394458No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG22381.493682882714525No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA22191.4810019288398262No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG21731.4503006720905554No Hit
TGTAAAANGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT21531.4369522995908723No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA20021.336172087218266No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT19191.2807763413445816No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG18991.2674279688448986No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG18871.2594189453450888No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT18351.2247131768459132No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG17301.1546342212225775No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA16961.1319419879731163No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG16931.129939732098164No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT16791.120595871348386No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC15011.0017953561012074No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC14550.9710940993519366No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC14350.9577457268522536No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC14280.9530737964773646No Hit
TGTAAAANGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT14220.9490692847274597No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC13340.8903364457288544No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC12750.8509587468547898No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT12660.8449519792299324No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT12630.8429497233549799No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGTCTTGGTGGT12540.8369429557301226No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGTCTTGGTGGT11590.7735381863566285No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT10830.7228143708578332No Hit
TGTAAAANGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT10550.7041266493582771No Hit
TGTAAAACGACGGCCAGTTCCCACCACCTGCCTTCCGGTCATAGCTGTTT10490.700122137608372No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC9880.659409601484339No Hit
TGTAAAANGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT9460.6313780192350048No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG9190.6133577163604328No Hit
TGTAAAANGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT9170.6120228791104645No Hit
TGTAAAANGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT9000.6006767624857339No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC8700.5806542037362095No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG8640.5766496919863046No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC8570.5719777616114154No Hit
TGTAAAANGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA8510.5679732498615107No Hit
TGTAAAACGACGGCCAGTTGCCTTTGTATCTCTGTATGAAGGTCATAGCT8410.5612990636116691No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT8400.5606316449866849No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT8110.5412765048621447No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA7990.5332674813623349No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7470.4985617128631592No Hit
TGTAAAANGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA6480.43248726898972845No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT6310.42114115236499794No Hit
TGTAAAANGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5900.3937769887406478No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT5830.38910505836575876No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA5560.37108475549118675No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGCCCGGTCAT5270.35172961536664643No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA5170.345055429116805No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC5010.33437673111705857No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4910.32770254486721706No Hit
TGTAAAANGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC4840.323030614492328No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGCCCGGTCAT4810.32102835861737555No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4720.31502159099251825No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4650.3103496606176292No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC4520.30167321849283524No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4410.29433161361800964No Hit
TGTAAAANGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC4400.2936641949930255No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC4360.2909945204930889No Hit
TGTAAAANGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4320.2883248459931523No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC4290.2863225901181998No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4250.2836529156182632No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC4030.26896970586861196No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA3990.26630003136867536No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC3990.26630003136867536No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC3950.2636303568687388No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC3780.25228424024400825No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA3650.24360779811921432No Hit
TGTAAAANGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC3610.24093812361927772No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA3600.24027070499429357No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT3540.23626619324438866No Hit
TGTAAAANGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA3400.2269223324946106No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT3390.22625491386962646No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA3270.21824589036981668No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA3230.21557621586988004No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3230.21557621586988004No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3110.20756719237007026No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3100.2068997737450861No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3080.20556493649511784No Hit
TGTAAAANGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG3060.20423009924514954No Hit
TGTAAAACGACGGCCAGTAGCCAGGCTAAGGGACCAGTGTCTCCCTGCCC2970.19822333162029218No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2940.19622107574533976No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2710.18087044737070432No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT2710.18087044737070432No Hit
TGTAAAANGACGGCCAGTGAGGCAGGTGTGCATCCTTAATTTTGGCAGGT2680.1788681914957519No Hit
TGTAAAANGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2660.1775333542457836No Hit
TGTAAAACGACGGCCAGTCCATTACTGCTGCGTCCCTGTTTTACAATGCA2640.1761985169958153No Hit
TGTAAAANGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2600.1735288424958787No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2590.17286142387089454No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2570.17152658662092624No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2550.17019174937095793No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2520.1681894934960055No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2520.1681894934960055No Hit
TGTAAAANGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2500.1668546562460372No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGCATCCTTAATTTTGGCAGGT2450.16351756312111645No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2440.1628501444961323No Hit
TGTAAAANGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG2390.15951305137121158No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT2370.15817821412124328No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2350.15684337687127498No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAGCC2260.15083660924641762No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG2250.15016919062143347No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT2240.14950177199644935No Hit
TGTAAAANGACGGCCAGTAGAACAATGCGGCCCCTGAAAGACAACAGGTC2230.1488343533714652No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG2200.14683209749651274No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT2190.1461646788715286No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG2120.14149274849663954No Hit
TGTAAAACGACGGCCAGTCAGCAGCAACTCCCTGTGCTCCCTTCTCTTTG2030.1354859808717822No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCCCCTGAAAGACAACAGGTC2020.13481856224679806No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG1960.13081405049689315No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1880.12547470149701997No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG1850.12347244562206754No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1850.12347244562206754No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1840.12280502699708337No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1820.12147018974711508No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1810.12080277112213092No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC1770.11813309662219433No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG1690.11279374762232115No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC1660.11079149174736871No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1630.10878923587241626No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA1580.10545214274749551No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)