FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235582
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA210588.938713484052261No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG100744.276218047219228No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG76693.2553420889541647No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT65632.7858664923466137No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTCTGGCATGG59992.5464594069156385No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC58902.5001910162915673No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG56152.3834588381115704No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA53172.256963605029247No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT43821.8600741992172578No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC36861.5646356682598839No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG35761.5179427969878854No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG33181.4084267898226521No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC31581.3405098861542901No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA28411.2059495207613486No Hit
TGTAAAACGACGGCCAGTGAGAGGGCAGGTGAGCATGGGCACCAGGTCAC27521.1681707430958224No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT27431.164350417264477No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC25801.0951600716523335No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT23821.0111129033627357No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA21560.9151802769311747No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCACCCCTCTCCACC21100.8956541671265207No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC20660.8769770186177213No Hit
TGTAAAACGACGGCCAGTGCCACACGAGGCTTCATTCGTTATGGCTGGCG19920.8455654506711039No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT18370.7797709502423785No Hit
TGTAAAACGACGGCCAGTTGCCCAACGTTGTAGTGTTGAGTGCATGGCAT14160.6010645974650015No Hit
TGTAAAACGACGGCCAGTGGCTCTCATCCTCTTCTGCAACTACTATGTTT13180.55946549396813No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA12800.543335229346894No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT12090.5131971033440585No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA12050.5114991807523496No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGAGCTCGGC10420.44230883514020597No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG8680.36844920240086254No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGCACATGGGT7080.30053229873250076No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC6700.28440203411126486No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC6690.2839775534633376No Hit
TGTAAAACGACGGCCAGTTGAAGAGAGAGGTTAGCAAGGGAGTAGTGCAG6670.2831285921674831No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA6230.26445144365868356No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC6210.26360248236282907No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTTCTGGGTC6160.26148007912319277No Hit
TGTAAAACGACGGCCAGTGCACAGAGGATGCCCCAACAAACTCATGGCGT6000.2546883887563566No Hit
TGTAAAACGACGGCCAGTAGGTGAAGAAGAAAAGACAAAGACCTACAGTG5470.23219091441621176No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAACACCTCCACGCAGGA5110.21690961109083035No Hit
TGTAAAACGACGGCCAGTGAGGCAGGTGTGTGGTCTGGGCAAAGCGAGAA4910.20841999813228518No Hit
TGAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGAG4880.20714655618850336No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG4880.20714655618850336No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA4860.20629759489264884No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC4810.20417519165301254No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGTGAACGAAG4710.19993038517373993No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGGCAATCATGGCT4650.19738350128617635No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC4620.19611005934239456No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG4590.1948366173986128No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCCAGGAGACAGG4350.18464908184835852No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCAAAGATTTT4230.17955531407323141No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG4120.17488602694603153No Hit
TGTAAAACGACGGCCAGTAGGCCTAGATTTCCTTCATGCCAATTGCATCG4100.17403706565017701No Hit
TGTAAAACGACGGCCAGTCGCACAGTCTGTTCTGCAGCCTGGGGAATGTC4070.17276362370639523No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGTAAACCTCCACGCAGGA3830.16257608815614097No Hit
TGNAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCTGAGTAGCCACAG3380.14347445899941422No Hit
TGTAAAACGACGGCTAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG3320.14092757511185067No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT3290.13965413316806885No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGACCGAGTAGCCACAG3170.13456036539294175No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCATAGCTGTT3010.12776867502610556No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTCCTTGACCCCGA3010.12776867502610556No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGCTCTGGTG2860.12140146530719666No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTGCTGGTGACAGG2800.11885458141963308No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTTCTTCTGGCTCT2780.11800562012377856No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACAGTCATAGT2740.11630769753206952No Hit
TGTAAAACGACGGCCAGTCCTGTGTTTCCACTTCTGCTTCCTGTGGGAGA2680.11376081364450595No Hit
TGTAAAACGACGGCCAGTGGGGAGTGAGAGGCCATAGCTGTTTCCTGTCA2670.11333633299657868No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCAGTGGGAGTCTGGCTAC2580.10951600716523334No Hit
TGTAAAACGACGGCCAGTCTTGCACTACATGGCCATCAGCTGTTTCCTGC2560.10866704586937881No Hit
TGTAAAACGACGGCCAGTATTCCTCCACTATCGCCCCTTGGGCAGGAATG2540.10781808457352429No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG2520.10696912327766976No Hit
TGAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAGC2400.10187535550254263No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG2370.10060191355876086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)