FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235582
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG105344.471479145265767No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT49382.0960854394648147No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC47392.01161379052729No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT39581.6800944044960993No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT37891.6083571749963919No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG37391.587133142600029No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA32991.4003616575120341No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT29891.2687726566545832No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC28291.2008557529862214No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC28141.1944885432673125No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT23841.0119618646585902No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG22590.9589017836676825No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT21250.9020213768454296No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC17430.7398697693372159No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC17030.7228905434201255No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT14620.6205907072696556No Hit
CAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT13970.5929994651543836No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG13020.5526738036012938No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA12610.535270097036276No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG12510.5310252905570035No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC11500.48815274511635015No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG11390.48348345798915027No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG11300.4796631321578049No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG10910.4631083868886417No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT10740.45589221587387835No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG10690.453769812634242No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT10420.44230883514020597No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG10360.43976195125264245No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC10310.4376395480130061No Hit
CAGGAAANAGCTATGANNAGAGGCACAGGTGGAGAGGGGTGATGGCCATG9940.4219337640396974No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG9220.3913711573889346No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT7760.32939698279155455No Hit
CAGGAAANAGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA7550.32048288918508205No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT7040.2988343761407917No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6970.29586301160530093No Hit
CAGGAAACAGCNATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG6530.27718586309650145No Hit
NAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6160.26148007912319277No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT6130.260206637179411No Hit
NAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG6100.25893319523562924No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT5970.2534149468125748No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG5890.2500191016291567No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG5790.24577429514988414No Hit
NAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5780.24534981450195686No Hit
CAGGANANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5760.24450085320610235No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG5630.23898260478304795No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC5470.23219091441621176No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG5320.22582370469730284No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT5200.2207299369221757No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCAGTGGGGCCAGG5190.22030545627424844No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4990.21181584331570324No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4810.20417519165301254No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT4740.20120382711752172No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG4690.19908142387788538No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4620.19611005934239456No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC4620.19611005934239456No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4490.1905918109193402No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG4480.19016733027141294No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT4270.18125323666494045No Hit
NAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4230.17955531407323141No Hit
CAGGAAANNGCTATGANNAGAGGCACAGGTGGAGAGGGGTGATGGCCATG4150.17615946888981332No Hit
CAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT4120.17488602694603153No Hit
CAGGAAANNGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG4000.1697922591709044No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3990.16936777852297713No Hit
CAGGANANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3870.16427401074785No Hit
NAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3860.16384953009992276No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA3800.16130264621235918No Hit
NAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3750.15918024297272287No Hit
NAGGAAANNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3700.15705783973308657No Hit
CAGGAAANNGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT3680.15620887843723205No Hit
NAGGANANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT3600.15281303325381396No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3560.1511151106621049No Hit
CAGGAAANAGCTATGANNGATGTAGCAGTGCTCTGGAGATCCTGCACTAC3500.14856822677454135No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3490.1481437461266141No Hit
NAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT3340.14177653640770518No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3330.1413520557597779No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3220.13668276863257806No Hit
CAGGAAANNGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3210.13625828798465078No Hit
NAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3200.1358338073367235No Hit
CAGGANANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3190.13540932668879624No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC3140.13328692344915996No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT3140.13328692344915996No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC3120.13243796215330542No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT3090.13116452020952366No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2980.12649523308232377No Hit
CAGGAAANNGCTATGANNTGGAAACCCATGCCATGCACTCAACACTACAA2970.12607075243439653No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2950.12522179113854198No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2930.12437282984268747No Hit
CAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2930.12437282984268747No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC2900.1230993878989057No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2900.1230993878989057No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2870.1218259459551239No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2820.11970354271548761No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2740.11630769753206952No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2740.11630769753206952No Hit
CAGGAAACAGCNATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2690.1141852942924332No Hit
CAGGAANNNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2680.11376081364450595No Hit
NAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2580.10951600716523334No Hit
CAGGAAANNGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2580.10951600716523334No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2550.10824256522145156No Hit
CAGGAAANAGCTATGANNACACATACTCCCTTGCTCCACCTTTCGACATT2550.10824256522145156No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2490.10569568133388799No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCACAGGAAACAGC2430.10314879744632442No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2430.10314879744632442No Hit
CAGGAAACAGCNATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT2420.10272431679839715No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2380.10102639420668813No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2370.10060191355876086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)