FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173919
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG75074.31637716408213No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA61523.5372788481994495No Hit
CAGGAAACAGCTATGACCGGGGAGACTGGGCCATACGATAGGCTTAATCC57163.2865874343803725No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT54903.1566418850154383No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC54003.104893657392234No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG51292.9490739942156985No Hit
CAGGAAACAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT51102.938149368384133No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT48122.7668052369206353No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC47582.735756300346713No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG41992.4143423087759244No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA40722.3413198097965147No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC35222.025080640988046No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT34171.9647077087609748No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA31521.8123379274259857No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG23241.3362542332925098No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT22221.277606241986212No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGACACTGCCCAC21461.233907738659951No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT21381.229307896204555No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA18681.0740632133349433No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT18481.0625636071964535No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG18381.0568138041272086No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA16700.9602171125638946No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG16500.9487175064254049No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACGGGGATGGCAGTG15340.8820197908221644No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA15110.8687952437629012No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGACACTGCCCACC14410.828546622278187No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA14120.8118721933773769No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT14070.8089972918427544No Hit
CAGGAAACAGCTATGACCCCCAAACCTGCGGAGGCACACAGCTGGGAGGC13540.7785233355757566No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT11840.6807766833985935No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA11300.6497277468246713No Hit
CAGGAAACAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA10150.5836050115283552No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA9700.5577308977167532No Hit
CAGGAAACAGCTATGACCAGAGGCACAGGTGGAGAGGGACGAGACCCTGA9670.5560059567959797No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA9000.5174822762320391No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC8890.5111574928558696No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT8770.5042577291727759No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTCAGGAAACAGCT8170.4697589107573065No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT8040.46228416676728823No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA7790.447909659094176No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT7200.41398582098563125No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG6680.3840868450255579No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGTGAGGACAGAT6560.37718708134246404No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGGTCTGGGTCCT5680.32658881433310905No Hit
CAGGAAACAGCTATGACCACAGGGGCCTCTTAAAACTGCATGTGTTCAGT4740.27254066548220723No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG4560.26219101995756644No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG4300.24724153197752977No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACGGGAAACCTCA4290.24666655167060528No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT4260.2449416107498318No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG4260.2449416107498318No Hit
CAGGAAACAGCTATGACCGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA4180.24034176829443593No Hit
CAGGAAACAGCTATGACCGCTGAGGCAGGACAATCGCTTGAACTTGGGAG3770.2167675757105319No Hit
CAGGAAACAGCTATGACCAGAGGGGCTCTGGGTTGAGGCCCCTGCCTACC3400.19549330435432588No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCAGAGGGGTC3240.18629361944353406No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCACAGGGACG3140.18054381637428918No Hit
CAGGAAACAGCTATGACCACCGTTAAGCCACACCAAATATGAATTTCTGT2900.16674428900810145No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC2870.16501934808732802No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC2760.15869456471115864No Hit
CAGGAAACAGCTATGACCTGCTCCCTGAGTTTAAGGGATTCAATTAGACT2730.15696962379038518No Hit
CAGGAAACAGCTATGACCACCTTTACCCAAATAGCTACCACCAAGAAATC2510.14432005703804643No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGACAGGAAACA2440.14029519488957504No Hit
CAGGAAACAGCTATGACCGGCCTTCTCTGCATTATCTCGCTCATAAGAGT2420.13914523427572606No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2330.13397041151340566No Hit
CAGGAAACAGCTATGACCACCTCTCTGGGCCTGTCTCCTCATCTGTAAAA2230.1282206084441608No Hit
CAGGAAACAGCTATGACCACACATACTCCCTTGCTCCAAACTTATAACAA2200.1264956675233873No Hit
CAGGAAACAGCTATGACCCCAGCAGAGCTCTTCTTACAGAGGTACTGTAA2110.12132084476106694No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCAAAACATTG2040.11729598261259551No Hit
CAGGAAACAGCTATGACCCACGACACCCCACCTCTTGGGCTCCGTGCTCT1990.11442108107797308No Hit
CAGGAAACAGCTATGACCCTGGGTGCCCTAGACCCAGTCATGTTTGTTGG1970.1132711204641241No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAATG1810.10407143555333229No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTA1770.10177151432563435No Hit
AAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1770.10177151432563435No Hit
CAGGAAACAGCTATGACCGGGCCTTCTCGAAGCTCTCCAGGGCCTTCTGG1750.10062155371178537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)