FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110067
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT12021.0920621076253554No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT10990.9984827423296718No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9980.9067204520882735No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9380.85220820045972No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT8850.8040557115211644No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT8640.7849764234511707No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8630.7840678859240282No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8020.7286470967683321No Hit
CAGGAAANAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT7460.6777689952483487No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC7200.6541470195426422No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA6620.6014518429683738No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT6530.5932750052240907No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG6330.5751042546812396No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG6040.5487566663941055No Hit
CAGGAAANAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC6000.5451225162855352No Hit
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5370.4878846520755539No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG5300.4815248893855561No Hit
CAGGAAACAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG5290.4806163518584135No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT5250.47698220174984324No Hit
CAGGAAACAGCTATGACCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC5200.47243951411413043No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5130.4660797514241326No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG5130.4660797514241326No Hit
CAGGAAANAGCTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG5040.45790291367984953No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC4930.4479090008812814No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC4900.44518338829985377No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4770.4333724004470005No Hit
CAGGAAANAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC4400.39975651194272577No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT4370.39703089936129815No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAAGTTG4290.3897625991441577No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG4280.3888540616170151No Hit
CAGGAAANAGCTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG4220.38340283645415973No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA4160.3779516112913044No Hit
CAGGAAANAGCTATGANNGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC4080.3706833110741639No Hit
CAGGAAACAGCTATGACCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC3890.3534210980584553No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3800.34524426031417227No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAATTTG3790.34433572278702973No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG3790.34433572278702973No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT3660.33252473493417645No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC3520.3198052095541806No Hit
CAGGAAANAGCTATGANCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3420.31071983428275507No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC3400.30890275922846994No Hit
CAGGAAANAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG3390.30799422170132734No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCAGAGCACC3340.3034515340656146No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3260.2961832338484741No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3230.29345762126704644No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA3190.2898234711584762No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3180.28891493363133364No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3170.2880063961041911No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3160.2870978585770485No Hit
CAGGAAANAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG3130.28437224599562083No Hit
CAGGAAANAGCTATGANNCAGGGTGACTCCGATCCTGGAGGACAAGGCGG3060.2780124833056229No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3050.2771039457784804No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2980.27074418308848247No Hit
CAGGAAANAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2960.26892710803419734No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2940.26711003297991226No Hit
CAGGAAANAGCTATGANCGTTTTCACTGGCTCCCGGGGCTGTTCACTGGC2930.26620149545276967No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2920.2652929579256271No Hit
CAGGAAANAGCTATGANNCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA2910.2643844203984846No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2890.26256734534419945No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC2850.25893319523562924No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2810.255299045127059No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2770.2516648950184887No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCGCTTCC2730.2480307449099185No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2710.2462136698556334No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2690.24439659480134826No Hit
CAGGAAACAGCTATGACCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG2670.24257951974706315No Hit
CAGGAAANAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA2630.23894536963849292No Hit
CAGGAAANAGCTATGACCGCTTCTATATGAGCGCATCTCACCAGAGCACC2610.2371282945842078No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2590.2353112195299227No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2570.23349414447563754No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2540.23076853189420987No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2520.22895145683992477No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2430.22077461909564172No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2430.22077461909564172No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2420.2198660815684992No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCTGAAGTTG2410.21895754404135664No Hit
CAGGAAANAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC2390.21714046898707154No Hit
CAGGAAACAGCTATGACCACAAACGCAAATGGCTTTTAGCATTTCACAGG2360.21441485640564384No Hit
CAGGAAANAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC2350.21350631887850127No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2330.21168924382421617No Hit
CAGGAAANAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG2320.2107807062970736No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2310.20987216876993103No Hit
CAGGAAANAGCTATGANCGTTTTCACTTGCTTCCGGGGCTGTTCACTGGC2310.20987216876993103No Hit
CAGGAAANAGCTATGACCGCTGAGGCAGGAGAATGGCGTGACTGGCCGTC2310.20987216876993103No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2290.20805509371564593No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2280.20714655618850336No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2270.20623801866136082No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2270.20623801866136082No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2250.20442094360707566No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2250.20442094360707566No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAATTTG2240.20351240607993315No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC2230.20260386855279056No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC2220.201695331025648No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2220.201695331025648No Hit
CAGGAAANAGCTATGANNTCAAGCAAGTTCAGGATGGTATAGAAATAGAG2190.19896971844422032No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2140.19442703080850754No Hit
CAGGAAANAGCTATGANNTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC2140.19442703080850754No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2110.19170141822707987No Hit
CAGGAAANAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA2100.1907928806999373No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2100.1907928806999373No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2080.1889758056456522No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2040.18534165553708196No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2010.18261604295565428No Hit
CAGGAAACAGCTATGACCGCTTCTATATGAGCGCATCTCACCAGAGCACC2000.18170750542851172No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1990.18079896790136918No Hit
CAGGAAANAGCTATGANCACAAACGCAAATGGCTTTTAGCATTTCACAGG1980.17989043037422658No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1960.1780733553199415No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1960.1780733553199415No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1950.17716481779279894No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1930.1753477427385138No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1910.1735306676842287No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1900.17262213015708613No Hit
CAGGAAANAGCTATGANNGCTGAGGCAGGAGAATGGCGTGACTGGCCGTC1870.16989651757565846No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1840.1671709049942308No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1830.16626236746708822No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1820.16535382993994568No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1810.16444529241280312No Hit
CAGGAAANAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1800.16353675488566055No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1800.16353675488566055No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1790.162628217358518No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCTGAAGTTG1780.16171967983137542No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1770.16081114230423288No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1740.1580855297228052No Hit
CAGGAAANAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1730.15717699219566264No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1710.15535991714137753No Hit
CAGGAAANAGCTATGANCCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1710.15535991714137753No Hit
CAGGAAACAGCTATGACCCCAGGAGAGAGCAGCCTTCCTCACACCCGTGA1700.15445137961423497No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1690.1535428420870924No Hit
CAGGAAANAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1690.1535428420870924No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1670.1517257670328073No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1670.1517257670328073No Hit
CAGGAAACAGCTATGACCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1640.14900015445137962No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1640.14900015445137962No Hit
CAGGAAANAGCTATGANCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT1620.1471830793970945No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1590.14445746681566682No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1560.14173185423423915No Hit
CAGGAAACAGCTATGACCGCTGAGGCAGGAGAATGGCGTGACTGGCCGTC1560.14173185423423915No Hit
CAGGAAACAGCTATGACCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1530.13900624165281145No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1510.13718916659852637No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTAT1510.13718916659852637No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC1510.13718916659852637No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1500.1362806290713838No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC1470.13355501648995613No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC1450.131737941435671No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1410.12810379132710076No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC1380.12537817874567309No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1360.12356110369138797No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1360.12356110369138797No Hit
CAGGAAACAGCTATGACCTCAAGCAAGTTCAGGATGGTATAGAAATAGAG1350.12265256616424541No Hit
CAGGAAANAGCTATGANCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1340.12174402863710286No Hit
CAGGAAANAGCTATGANCGCTTCTATATGAGCGCATCTCACCAGAGCACC1320.11992695358281774No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1270.11538426594710494No Hit
CAGGAAANAGCTATGANCCAGGGTGACTCCGATCCTGGAGGACAAGGCGG1250.11356719089281983No Hit
CAGGAAANAGCTATGANCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC1250.11356719089281983No Hit
CAGGAAACAGCTATGACCTCCTCTTCATTTTCACGGTGCCATTCTGATTC1250.11356719089281983No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1240.11265865336567726No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1230.11175011583853471No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1210.1099330407842496No Hit
CAGGAAANAGCTATGANCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1190.10811596572996447No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1180.10720742820282192No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1170.10629889067567935No Hit
CAGGAAANAGCTATGACCCTGGCCTCCTTCAATGCATTTAATGGCCTTCT1160.1053903531485368No Hit
CAGGAAANAGCTATGANCAACCAACACTGCTGCTAATTGTGTTTGTGTCC1160.1053903531485368No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1160.1053903531485368No Hit
CAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1150.10448181562139425No Hit
CAGGAAANAGCTATGANCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1120.10175620303996658No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1120.10175620303996658No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1120.10175620303996658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)