FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103998
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC13191.2682936210311737No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT12151.1682916979172677No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC10330.9932883324679321No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC10290.9894421046558587No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA10270.9875189907498222No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA10260.9865574337968037No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG10070.9682878516894555No Hit
CAGGAAANAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT8600.8269389795957615No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA8560.8230927517836881No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC8190.7875151445220101No Hit
CAGGAAANAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC7640.734629512106002No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG6950.6682820823477374No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA6670.6413584876632243No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG6650.6394353737571876No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6590.6336660320390777No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC6490.6240504625088944No Hit
CAGGAAACAGCTATGACCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6250.6009730956364545No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA6230.5990499817304179No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT5750.5528952479855382No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5690.5471259062674283No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5510.5298178811130984No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT5390.5182791976768784No Hit
CAGGAAANAGCTATGANNACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA5330.5125098559587684No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT5310.5105867420527318No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC5070.4875093751802919No Hit
CAGGAAANAGCTATGACCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA5060.4865478182272736No Hit
CAGGAAANAGCTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4890.4702013500259621No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4880.4692397930729437No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC4850.46635512221388875No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG4800.4615473374487971No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4740.45577799573068717No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG4710.45289332487163214No Hit
CAGGAAANAGCTATGANCCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4400.423085059328064No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG4320.41539260370391734No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA4300.41346948979788073No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT4270.4105848189388257No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT4260.40962326198580745No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA4250.4086617050327891No Hit
CAGGAAANAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT4070.3913536798784592No Hit
CAGGAAANAGCTATGANCAGAGACCAGATGCATAAGCACAGCAGCATTCC4010.38558433816034926No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3970.3817381103482759No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3960.3807765533952576No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA3930.3778918825362026No Hit
CAGGAAANAGCTATGANNCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3890.3740456547241293No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3860.37116098386507435No Hit
CAGGAAANAGCTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3740.3596223004288544No Hit
CAGGAAACAGCTATGACCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA3710.3567376295697994No Hit
CAGGAAANAGCTATGANNCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG3690.35481451566376276No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3610.34712206003961615No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3600.3461605030865978No Hit
CAGGAAANAGCTATGANNGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC3590.3451989461335795No Hit
CAGGAAANAGCTATGANNTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA3470.33366026269735954No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3440.3307755918383046No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG3340.3211600223081213No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3270.314429123636993No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3220.3096213388719014No Hit
CAGGAAACAGCTATGACCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT3080.2961595415296448No Hit
CAGGAAACAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3080.2961595415296448No Hit
CAGGAAANAGCTATGANNGTCAACAGCATACCTGTACACCTGAAATAACT3060.29423642762360813No Hit
CAGGAAANAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC3040.29231331371757147No Hit
CAGGAAACAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT3030.29135175676455316No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2960.2846208580934249No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2880.27692840246927825No Hit
CAGGAAANAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2820.27115906075116825No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTCACCATCCAACT2810.27019750379814994No Hit
CAGGAAANAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2780.26731283293909497No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTCACCATCCAACT2760.26538971903305836No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2750.26442816208004No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC2730.26250504817400333No Hit
CAGGAAANAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA2730.26250504817400333No Hit
CAGGAAANAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG2700.25962037731494836No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC2680.2576972634089117No Hit
CAGGAAANAGCTATGANCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG2660.2557741495028751No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT2570.2471201369257101No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2520.24231235216061847No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2450.23558145348949017No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2380.2288505548183619No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2380.2288505548183619No Hit
CAGGAAANAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC2340.22500432700628858No Hit
CAGGAAANAGCTATGANCACTCCCTTGAGCCTTTGGGGCTGTTGTCACTA2330.22404277005327028No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT2330.22404277005327028No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT2230.214427200523087No Hit
CAGGAAANAGCTATGANCAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT2210.21250408661705034No Hit
CAGGAAANAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG2170.20865785880497703No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA2150.20673474489894036No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTCACCATCCAACT2120.20385007403988537No Hit
CAGGAAANAGCTATGANCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2120.20385007403988537No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT2110.20288851708686706No Hit
CAGGAAANAGCTATGANNGGTAGCAACGTCCCACCCAGCTGAATAGTTGT2110.20288851708686706No Hit
CAGGAAACAGCTATGACCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2030.19519606146272045No Hit
CAGGAAANAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2020.1942345045097021No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG1990.19134983365064714No Hit
CAGGAAANAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1970.18942671974461048No Hit
CAGGAAACAGCTATGACCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1950.1875036058385738No Hit
CAGGAAACAGCTATGACCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1950.1875036058385738No Hit
CAGGAAACAGCTATGACCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1940.1865420488855555No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1920.18461893497951884No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1830.1759649224023539No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1750.16827246677820729No Hit
CAGGAAACAGCTATGACCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1740.16731090982518892No Hit
CAGGAAANAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1690.1625031250600973No Hit
CAGGAAANAGCTATGANNTTGCCAGTGCTGCCTTCAGCCATTTGTGAAAT1620.155772226388969No Hit
CAGGAAACAGCTATGACCTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA1610.1548106694359507No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1610.1548106694359507No Hit
CAGGAAANAGCTATGANCGCCCCTACTCCAAAAGGCGGATGAATGGGGAA1600.15384911248293237No Hit
CAGGAAANAGCTATGANCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1590.15288755552991404No Hit
CAGGAAANAGCTATGANCCCCGGAACATGCTTCTGCCCTTAGGAAAGTGC1550.14904132771784073No Hit
CAGGAAACAGCTATGACCGTCAACAGCATACCTGTACACCTGAAATAACT1510.14519509990576743No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1500.1442335429527491No Hit
CAGGAAANAGCTATGANNTCCCTGCTTGATAATGACCCCTCTGAATTCTT1500.1442335429527491No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1480.14231042904671246No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1450.13942575818765746No Hit
CAGGAAACAGCTATGACCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1430.13750264428162082No Hit
CAGGAAANAGCTATGANCGCTGCTGACTGCCTGGGGGCTCCTGTGCACGC1420.13654108732860248No Hit
CAGGAAANAGCTATGANCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1410.13557953037558412No Hit
CAGGAAANAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1410.13557953037558412No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1400.13461797342256582No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1380.13269485951652918No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1380.13269485951652918No Hit
CAGGAAANAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1340.12884863170445585No Hit
CAGGAAACAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1320.1269255177984192No Hit
NAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1320.1269255177984192No Hit
CAGGAAANAGCTATGANCTCCAGCTGCTCCATCTTGTCTACCTCCTGGAA1310.12596396084540087No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1300.12500240389238254No Hit
CAGGAAANAGCTATGACCTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA1300.12500240389238254No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA1290.12404084693936421No Hit
CAGGAAANAGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1280.1230792899863459No Hit
CAGGAAANAGCTATGANNGCCTCTCAGGGCCTCACAGTGAAGCTCCAGCA1270.12211773303332757No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTCACCATCCAACT1250.12019461912729092No Hit
CAGGAAANAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1250.12019461912729092No Hit
CAGGAAANAGCTATGANCCCCTCCGTCTGCAGGGGAAACAAGAGGACCAG1230.11827150522125426No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1220.11730994826823593No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1210.1163483913152176No Hit
CAGGAAACAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA1200.11538683436219928No Hit
CAGGAAANAGCTATGANCGTCCTAGCACCGCAGCGAATGGGACCCGCAAG1200.11538683436219928No Hit
CAGGAAANAGCTATGACCTCCCTGCTTGATAATGACCCCTCTGAATTCTT1190.11442527740918094No Hit
CAGGAAANAGCTATGANNAGGACCACCCCTCCATCCTGGCCAGAGGGGTC1190.11442527740918094No Hit
CAGGAAANAGCTATGANNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1180.11346372045616263No Hit
CAGGAAANAGCTATGANCCAGAAGGCTGAGCAGGAGGCTGGGAAAAAGGG1170.11250216350314429No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1160.11154060655012596No Hit
CAGGAAANAGCTATGACCGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC1150.11057904959710764No Hit
CAGGAAANAGCTATGANCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1140.1096174926440893No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT1130.10865593569107097No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1120.10769437873805265No Hit
CAGGAAACAGCTATGANCAGAGACCAGATGCATAAGCACAGCAGCATTCC1100.105771264832016No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGGACGGTCAAACTGGCCGTC1100.105771264832016No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1090.10480970787899767No Hit
NAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1080.10384815092597934No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA1070.10288659397296102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)