FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84541
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC23232.747779184064537No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC13481.5944926130516555No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT13191.5601897304266568No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG12631.4939496812197632No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC12291.4537325084870063No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC10841.2822180953620137No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC10251.212429472090465No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG9751.1532865710128812No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC9531.1272636945387444No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA8951.0586579292887475No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC8941.0574750712671959No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT8811.042097916987024No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC8581.0148921824913357No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG8100.9581149974568554No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG7650.9048863864870299No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC7470.8835949420990998No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC7010.829183473107723No Hit
TGTAAAANGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC7010.829183473107723No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA6850.8102577447628961No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC6730.796063448504276No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC5600.6624004920689369No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT5450.6446576217456619No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT5440.6434747637241102No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC5360.6340118995516968No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT5030.5949775848404916No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC5010.5926118687973883No Hit
TGTAAAACGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT5000.5914290107758365No Hit
TGTAAAACGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC4940.5843318626465265No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4920.5819661466034233No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT4890.5784175725387682No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT4740.5606747022154931No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGATGAAC4700.5559432701292863No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT4450.5263718195904945No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG4320.5109946653103228No Hit
TGTAAAANGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC4240.5015318011379094No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT4160.49206893696549603No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG4150.49088607894394437No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC4070.48142321477153094No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG3940.4660460604913592No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTGGGCTCACT3900.4613146284051526No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGCGCAGCCCTCCTAC3810.4506689062111875No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG3790.4483031901680841No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3760.4447546161034291No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC3740.4423889000603257No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC3660.4329260358879124No Hit
TGTAAAANGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC3600.4258288877586024No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG3590.4246460297370506No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC3570.42228031369394736No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG3550.419914597650844No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG3530.4175488816077406No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG3470.41045173347843056No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG3370.39862315326291387No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG3340.39507457919825884No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC3330.3938917211767072No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT3100.36668598668101865No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC3090.365503128659467No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA3090.365503128659467No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG3060.36195455459481196No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG3040.35958883855170864No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG3020.35722312250860533No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT2940.3477602583361919No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG2870.3394802521853302No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCGGCTCACT2700.3193716658189517No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC2670.31582309175429674No Hit
TGTAAAACGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT2660.3146402337327451No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG2640.3122745176896417No Hit
TGTAAAANGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC2580.3051773695603317No Hit
TGTAAAANGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG2530.2992630794525733No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC2530.2992630794525733No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG2440.28861735725860826No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA2420.2862516412155049No Hit
TGTAAAANGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2390.28270306715084986No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC2380.2815202091292982No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTGGGGAGGCAG2370.28033735110774655No Hit
TGTAAAANGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC2370.28033735110774655No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC2290.2708744869353331No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC2130.2519487585905064No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG2100.2484001845258514No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT2030.24012017837498964No Hit
TGTAAAANGACGGCCAGTGAGACAGGCTTCTGGGCTGTCCACAACTCTCT1970.2330230302456796No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTACCCAGTGGTGTG1970.2330230302456796No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC1920.22710874013792126No Hit
TGTAAAACGACGGCCAGTAGAACAATGCGGCACTGAAGGAAGAGATGATT1880.22237730805171455No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC1870.22119445003016286No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC1840.21764587596550786No Hit
TGTAAAACGACGGCCAGTGGGCTGGATGGGCACAGGTGACCCTTCCAGGA1830.2164630179439562No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGATCAGACG1810.21409730190085283No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT1780.2105487278361978No Hit
TGTAAAANGACGGCCAGTGGGGTGGGGAGTGAGATGGTCCTAGGAAGTAC1770.20936586981464617No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT1740.20581729574999114No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC1740.20581729574999114No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC1720.20345157970688776No Hit
TGTAAAANGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT1720.20345157970688776No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT1710.20226872168533613No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA1700.20108586366378445No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA1660.19635443157757773No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGTTCGGAGGCAGG1630.19280585751292273No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC1600.1892572834482677No Hit
TGTAAAANGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG1590.188074425426716No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA1590.188074425426716No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC1580.18689156740516435No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC1550.18334299334050932No Hit
TGTAAAACGACGGCCAGTACTTCCCAGTTTCCAGTCCCCATTTCTCCTTC1510.17861156125430266No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC1490.1762458452111993No Hit
TGTAAAACGACGGCCAGTACAGCTTGGCACAGGCTCTCTTGTGACAATGA1480.17506298718964763No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA1480.17506298718964763No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTCCACTCACT1470.17388012916809595No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTATGGGCTCACT1450.1715144131249926No Hit
TGTAAAACGACGGCCAGTGGATCCAGGTAGGTGTCCAGGTCACTGCCAAT1430.16914869708188926No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCAGTGGG1400.16560012301723423No Hit
TGTAAAANGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT1390.16441726499568257No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGGCCCTGCT1390.16441726499568257No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGGTCTGTGCGG1360.16086869093102754No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC1360.16086869093102754No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG1340.1585029748879242No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTGGCTGAGAGCCCCCTGCCG1330.15732011686637254No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC1320.15613725884482085No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTTGGCTCACT1310.15495440082326917No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1290.15258868478016585No Hit
TGTAAAANGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC1270.1502229687370625No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT1260.14904011071551082No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGCGCGGGCAC1250.14785725269395913No Hit
TGTAAAANGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG1250.14785725269395913No Hit
TGTAAAANGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC1240.14667439467240748No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG1220.14430867862930413No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1180.13957724654309744No Hit
TGTAAAANGACGGCCAGTTGATGGACTGGATGCTGCCGTCTGGGCTCACT1140.13484581445689076No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA1130.13366295643533907No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG1130.13366295643533907No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC1110.13129724039223573No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG1100.13011438237068404No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAG1090.12893152434913238No Hit
TGTAAAANGACGGCCAGTGCCCAAGGTTGGTGAGATGGGAACCACCCAGC1070.126565808306029No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC1070.126565808306029No Hit
TGTAAAANGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTTCCTATCG1060.12538295028447735No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG1030.12183437621982234No Hit
TGTAAAANGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG1020.12065151819827066No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG1020.12065151819827066No Hit
TGTAAAANGACGGCCAGTGGTGGAGGCCCATTGAGGATGGCCGAGCAGGG1020.12065151819827066No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT990.11710294413361565No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCTCTTTCTCTAG990.11710294413361565No Hit
TGTAAAANGACGGCCAGTCCACTGCATGGCTACTGAATGCTCACCACAAT980.11592008611206396No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCATCCTCCTCTTC970.1147372280905123No Hit
TGTAAAANGACGGCCAGTTGTGTGTTCCTGCTCCTGGCACTGCAGCACGC970.1147372280905123No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG960.11355437006896063No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG950.11237151204740894No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC940.11118865402585727No Hit
TGTAAAANGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG930.11000579600430561No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTTAGAAGCAGAC910.10764007996120226No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTA910.10764007996120226No Hit
TGTAAAACGACGGCCAGTTGATGGACTGGATGCTGCCGCCATCTCTGCTC900.1064572219396506No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG880.10409150589654724No Hit
TGTAAAACGACGGCCAGTGACTGTAGGTTGGGCTCCCCCACAGGATCCTC880.10409150589654724No Hit
TGTAAAACGACGGCCAGTGCCAATCCTGAGGCCATCAAAGTGACAGGTAG880.10409150589654724No Hit
TGTAAAANGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG880.10409150589654724No Hit
TGTAAAACGACGGCCAGTGAACAGCCCCGGAAGCAAGTAAAAGGAGGAGG850.10054293183189222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)