FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84541
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6340.7499319856637608No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4640.5488461219999763No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4580.5417489738706662No Hit
NNGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC4580.5417489738706662No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4560.539383257827563No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3950.4672289185129109No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3670.43410889390946406No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT3570.42228031369394736No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3550.419914597650844No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3340.39507457919825884No Hit
NNGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3310.3915260051336038No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3300.39034314711205215No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3250.38442885700429374No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3050.3607716965732603No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2980.3524916904223986No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2950.34894311635774355No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2860.3382973941637785No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2850.3371145361422268No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA2690.3181888077974001No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA2660.3146402337327451No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2640.3122745176896417No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2560.30281165351722833No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2540.300445937474125No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2510.29689736340947No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2490.2945316473663666No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2470.29216593132326324No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2420.2862516412155049No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC2400.2838859251724016No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2340.2767887770430915No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2330.27560591902153986No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2310.27324020297843654No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA2250.2661430548491265No Hit
NNGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2100.2484001845258514No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2060.24366875243964467No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA2050.24248589441809298No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1990.23538874628878295No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1980.2342058882672313No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1950.2306573142025763No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1900.2247430240948179No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1850.21882873398705954No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1840.21764587596550786No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1820.2152801599224045No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1780.2105487278361978No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1770.20936586981464617No Hit
NNGGANNNNGNTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1760.20818301179309448No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1740.20581729574999114No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1730.20463443772843945No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1670.19753728959912942No Hit
NAGGANNNNGNTATGNNNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1670.19753728959912942No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1600.1892572834482677No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1550.18334299334050932No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1540.18216013531895767No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1500.17742870323275095No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1490.1762458452111993No Hit
NAGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1490.1762458452111993No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1480.17506298718964763No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1470.17388012916809595No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1470.17388012916809595No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1470.17388012916809595No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1440.17033155510344092No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1430.16914869708188926No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1420.1679658390603376No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1390.16441726499568257No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC1360.16086869093102754No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1340.1585029748879242No Hit
NAGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1330.15732011686637254No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1330.15732011686637254No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1320.15613725884482085No Hit
NAGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1310.15495440082326917No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1300.1537715428017175No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1290.15258868478016585No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1240.14667439467240748No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1220.14430867862930413No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1220.14430867862930413No Hit
NAGGANNNNGNTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC1210.14312582060775245No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC1200.1419429625862008No Hit
NAGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1190.1407601045646491No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1190.1407601045646491No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCAGAGAGTGATAT1190.1407601045646491No Hit
NAGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1170.13839438852154576No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1160.13721153049999407No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA1150.13602867247844241No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1130.13366295643533907No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1130.13366295643533907No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1100.13011438237068404No Hit
NNGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1100.13011438237068404No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1100.13011438237068404No Hit
NAGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1090.12893152434913238No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1090.12893152434913238No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1090.12893152434913238No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCAGACGGC1070.126565808306029No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1060.12538295028447735No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1060.12538295028447735No Hit
NAGGANNNNGNTATGNNNACACCCGGAGCAGTGCAAATCAACTCCCCCAG1050.1242000922629257No Hit
NNGGANNNNGNTATGNNNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1040.12301723424137401No Hit
NAGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1030.12183437621982234No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1030.12183437621982234No Hit
NNGGANNNNGNTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1030.12183437621982234No Hit
NNGGANNNNGNTATGANNGGTAGCAACGTCCCACCCAGGGCACGGGAACA1020.12065151819827066No Hit
NAGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1010.11946866017671898No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1010.11946866017671898No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1010.11946866017671898No Hit
NAGGANNNAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1000.11828580215516732No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1000.11828580215516732No Hit
NAGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA980.11592008611206396No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT980.11592008611206396No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA980.11592008611206396No Hit
NAGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT980.11592008611206396No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT950.11237151204740894No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT950.11237151204740894No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA950.11237151204740894No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCACAGGTCCCACCC940.11118865402585727No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC930.11000579600430561No Hit
NNGGANNNNGNTATGANNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG930.11000579600430561No Hit
NAGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT930.11000579600430561No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT930.11000579600430561No Hit
NNGGANNNNGNTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC920.10882293798275393No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG920.10882293798275393No Hit
NAGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT910.10764007996120226No Hit
NAGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT890.1052743639180989No Hit
NAGGANNNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT880.10409150589654724No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC870.10290864787499557No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC870.10290864787499557No Hit
NNGGANNNNGNTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT870.10290864787499557No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA860.10172578985344388No Hit
NAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC860.10172578985344388No Hit
NNGGANNNNGNTATGNNNTCTGACTGTCCGTGATCCCACACAATCACCCT850.10054293183189222No Hit
NAGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA850.10054293183189222No Hit
NNGGANNNNGNTATGANNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC850.10054293183189222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)