FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177402
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT12050.6792482610117135No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT8850.4988669800791423No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC8820.4971759055703994No Hit
NNGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8370.4718097879392566No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC7930.44700736181102807No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC7630.4300966167235995No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT7270.40980372261868525No Hit
NNGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6870.3872560625021138No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA6780.38218283897588523No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6570.3703453174146853No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA6210.35005242330977104No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT6210.35005242330977104No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6180.3483613488010282No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT6140.346106582789371No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5480.3089029435970282No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5480.3089029435970282No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT5430.3060844860824568No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT5340.30101126255622823No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC5150.29030112400085684No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC5130.28917374099502824No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5120.28861004949211394No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4950.27902729394257114No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4850.27339037891342827No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA4790.27000822989594253No Hit
NAGGANNNNGNTATGNNNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT4770.268880846890114No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA4700.264935006369714No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT4560.257043325328914No Hit
NNGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG4480.2525337933055997No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC4470.2519701018026854No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC4300.2423873462531426No Hit
NNGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA4280.241259963247314No Hit
NNGGANNNNGNTATGNNNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC4170.23505935671525685No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT4160.2344956652123426No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4150.2339319737094283No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4130.23280459070359974No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT4060.22885875018319973No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC3970.22378552665697116No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3930.22153076064531405No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3840.21645753711908547No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT3820.2153301541132569No Hit
NNGGANNNNGNTATGNNNCTGGAATTTCAACAGAATGGCATTTATATGAT3720.20969323908411405No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT3720.20969323908411405No Hit
NNGGANNNNGNTATGNNNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG3710.20912954758119973No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA3690.20800216457537118No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3670.20687478156954262No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT3660.20631109006662832No Hit
NNGGANNNNGNTATGANNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA3550.2001104835345712No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3530.19898310052874263No Hit
NNGGANNNNGNTATGANNTCCCTTAAACTTGGGATTGCTCCATTGCACTC3380.19052772798502834No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3340.1882729619733712No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG3330.18770927047045693No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3300.18601819596171407No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3290.1854545044587998No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3250.18319973844714263No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA3190.17981758942965695No Hit
NAGGANNNNGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT3160.17812651492091408No Hit
NNGGANNNNGNTATGANNGTCCTCAGATAGCCAGGCTCTGGAGTGCAGTG3040.17136221688594266No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2970.16741637636554266No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2970.16741637636554266No Hit
NNGGANNNNGNTATGANNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT2860.16121576983348554No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC2850.16065207833057124No Hit
NNGGANNNNGNTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC2840.16008838682765697No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2820.1589610038218284No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2800.15783362081599983No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2790.15726992931308553No Hit
NNGGANNNNGNTATGANNGTCCTAGCACCGCAGCGAATGGGACCCGCAAG2780.15670623781017123No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2760.1555788548043427No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2760.1555788548043427No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT2720.15332408879268555No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG2690.15163301428394269No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2650.14937824827228555No Hit
NAGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2640.14881455676937125No Hit
NNGGANNNNGNTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT2610.1471234822606284No Hit
NAGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2530.14261395023731413No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC2530.14261395023731413No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC2480.13979549272274272No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC2450.13810441821399985No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC2430.13697703520817128No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2430.13697703520817128No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2420.13641334370525698No Hit
NNGGANNNNGNTATGNNNGGTAGCAACGTCCCACCCAGGGCACGGGAACA2420.13641334370525698No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2400.13528596069942841No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA2390.13472226919651414No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCGAGACGGC2340.1319038116819427No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAGGCCGCAGCTCC2330.1313401201790284No Hit
NNGGANNNNGNTATGANNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT2290.1290853541673713No Hit
NAGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2280.128521662664457No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2270.1279579711615427No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2260.12739427965862843No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT2230.12570320514988556No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG2230.12570320514988556No Hit
NNGGANNNNGNTATGNNNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG2210.124575822144057No Hit
NAGGANNNNGNTATGNNNGTCCTCAGATAGCCAGGCTCTAGCAATTCTCC2210.124575822144057No Hit
NNGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2200.1240121306411427No Hit
NAGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2160.12175736462948558No Hit
NNGGANNNNGNTATGNNNACCTCTCTGGGCCTGTCTCCTCCCCGCCAAGA2130.12006629012074271No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA2130.12006629012074271No Hit
NNGGANNNNGNTATGNNNGCTCCTGTGCTGTCAAGGGACCCCGCCATTCA2120.11950259861782844No Hit
NNGGANNNNGNTATGNNNAAACCCTCGACCCTGAGGCTGGAAGCGCCCCA2100.11837521561199986No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2100.11837521561199986No Hit
NNGGANNNNGNTATGNNNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2090.11781152410908557No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2070.116684141103257No Hit
NAGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2010.11330199208577131No Hit
NAGGANNNNGNTATGNNNACGTTCTGGGCTGTGGTGTGGGCACCAGGCGA1970.11104722607411414No Hit
NNGGANNNNGNTATGNNNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1940.10935615156537132No Hit
NAGGANNNAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1940.10935615156537132No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1940.10935615156537132No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1930.10879246006245702No Hit
NNGGANNNNGNTATGNNNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1930.10879246006245702No Hit
NNGGANNNNGNTATGNNNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1910.10766507705662845No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1880.1059740025478856No Hit
NAGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1870.1054103110449713No Hit
NAGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1860.10484661954205703No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC1850.10428292803914274No Hit
NNGGANNNNGNTATGNNNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG1840.10371923653622846No Hit
NNGGANNNNGNTATGNNNGCCTCTCAGGGCCTCACAGTAGGAGGGCTGCG1830.10315554503331416No Hit
NAGGANNNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1830.10315554503331416No Hit
NAGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1810.1020281620274856No Hit
NNGGANNNNGNTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG1810.1020281620274856No Hit
NNGGANNNNGNTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1810.1020281620274856No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA1790.10090077902165703No Hit
NNGGANNNNGNTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1780.10033708751874275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)