FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93196
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA5640.6051761878192197No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA4020.43134898493497575No Hit
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT3770.40452379930469123No Hit
NNGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3410.3658955319970814No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC3320.356238465170179No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3260.3498004206189107No Hit
NAGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3050.32726726468947165No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT2980.319756212712992No Hit
NNGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2820.30258809390960983No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2780.2982960642087643No Hit
NNGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2700.28971200480707326No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2700.28971200480707326No Hit
NNGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC2670.2864929825314391No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT2630.2822009528305936No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2450.2628868191767887No Hit
NNGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2260.24249967809777243No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT2200.23606163354650414No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2190.23498862612129273No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC2140.22962358899523586No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2080.22318554444396757No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2020.21674749989269929No Hit
NNGGANNNNGNTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1970.21138246276664235No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT1940.20816344049100818No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1890.20279840336495128No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1880.2017253959397399No Hit
NAGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1870.20065238851452852No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1840.19743336623889435No Hit
NAGGANNNNGNTATGNNNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1830.196360358813683No Hit
NNGGANNNNGNTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1830.196360358813683No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1820.19528735138847161No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1810.19421434396326023No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1810.19421434396326023No Hit
NNGGANNNNGNTATGNNNAGGCACCAACAGTACAAAGCAGATACAAAGCA1790.19206832911283744No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1780.19099532168762606No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1750.18777629941199192No Hit
NNGGANNNNGNTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1740.18670329198678054No Hit
NAGGANNNNGNTATGNNNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1690.18133825486072364No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1670.17919224001030087No Hit
NNGGANNNNGNTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1650.1770462251598781No Hit
NNGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1640.17597321773466673No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1630.17490021030945535No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1620.17382720288424397No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1600.1716811880338212No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1560.16738915833297568No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1560.16738915833297568No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1540.16524314348255292No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1530.16417013605734151No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1480.1588050989312846No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1460.15665908408086182No Hit
NNGGANNNNGNTATGNNNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1460.15665908408086182No Hit
NNGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT1420.1523670543800163No Hit
NNGGANNNNGNTATGNNNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1400.15022103952959354No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1390.14914803210438216No Hit
NNGGANNNNGNTATGNNNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1380.14807502467917077No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1350.14485600240353663No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT1320.1416369801279025No Hit
NNGGANNNNGNTATGNNNGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT1300.13949096527747973No Hit
NNGGANNNNGNTATGANNACTCCCAACTCCCGTCTGGTCGGGGCAAAAAC1290.13841795785226835No Hit
NAGGANNNNGNTATGNNNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC1290.13841795785226835No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1270.13627194300184559No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1270.13627194300184559No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1270.13627194300184559No Hit
NNGGANNNNGNTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1240.13305292072621142No Hit
NAGGAANNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1240.13305292072621142No Hit
NNGGAAANNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1230.13197991330100003No Hit
NNGGANNNNGNTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1230.13197991330100003No Hit
NAGGANNNNGNTATGNNNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1220.13090690587578865No Hit
NNGGANNNNGNTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1220.13090690587578865No Hit
NNGGANNNNGNTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA1220.13090690587578865No Hit
NAGGANNNNGNTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1210.12983389845057727No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1210.12983389845057727No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1200.1287608910253659No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC1200.1287608910253659No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1200.1287608910253659No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1200.1287608910253659No Hit
NAGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1190.1276878836001545No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1170.12554186874973175No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1170.12554186874973175No Hit
NAGGAAANAGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1160.12446886132452037No Hit
NAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1150.12339585389930897No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1140.1223228464740976No Hit
NAGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1140.1223228464740976No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA1120.12017683162367483No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1110.11910382419846345No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1110.11910382419846345No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1100.11803081677325207No Hit
NNGGANNNNGNTATGNNNTGGGCCTGGACACTTTGACGGCGGGGCACGTC1100.11803081677325207No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1090.11695780934804069No Hit
NNGGANNNNGNTATGNNNCCTCCGTCCCAGCCTGATTTCAGCCCTGGGTT1060.11373878707240655No Hit
NNGGANNNNGNTATGANNCATCAGGCCCCACCTCCAACCCCGTTAGCTGC1050.11266577964719517No Hit
NAGGANNNNGNTATGNNNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1050.11266577964719517No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1040.11159277222198379No Hit
NAGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1040.11159277222198379No Hit
NNGGANNNNGNTATGANNAGGGCAAGTGTTCGCGTTGTGACAGTTACGGT1040.11159277222198379No Hit
NAGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1030.1105197647967724No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1020.10944675737156102No Hit
NNGGANNNNGNTATGNNNTCCCTTAAACTTGGGATTGCTCCATTGCACTC1000.10730074252113825No Hit
NNGGANNNNGNTATGNNNACAGCTTTCTGAGGGTTGTTCCAGGTGCGGTG1000.10730074252113825No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC990.10622773509592687No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC990.10622773509592687No Hit
NNGGANNNNGNTATGNNNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC980.10515472767071549No Hit
NNGGANNNNGNTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT960.10300871282029271No Hit
NAGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT950.10193570539508133No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT940.10086269796986995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)