FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104363
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT12121.1613311230991825No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT11711.1220451692649693No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA10641.0195184116976321No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT10270.9840652338472446No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT9680.9275317880858159No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA8390.8039247626074376No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT8000.7665551967651371No Hit
CAGGAAANAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT7990.7655970027691806No Hit
CAGGAAANAGCTATGANNGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT7410.7100217510037082No Hit
CAGGAAANAGCTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT7350.7042725870279697No Hit
CAGGAAANAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA7310.700439811044144No Hit
CAGGAAANAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT6910.6621120512058871No Hit
CAGGAAANAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT6770.6486973352624973No Hit
CAGGAAANAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT6330.6065367994404147No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT6060.5806655615495913No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA5970.5720418155859835No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA5910.566292651610245No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAACT5780.5538361296628116No Hit
CAGGAAACAGCTATGACCGCATCAGATTCACTTCCACTGTCGTCAGAATT5540.5308394737598574No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT5230.5011354598852084No Hit
CAGGAAACAGCTATGACCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT5190.49730268390138266No Hit
CAGGAAANAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT4920.4714314460105593No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA4880.4675986700267336No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC4780.45801673006716936No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT4760.45610034207525657No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4630.4436438201278231No Hit
CAGGAAANAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAATT4570.43789465615208456No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4180.4005250903097841No Hit
CAGGAAACAGCTATGACCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4020.3851939863744814No Hit
CAGGAAANAGCTATGANCGGGACCAGTCCAATAACCCCTGAGGGGTATAT4020.3851939863744814No Hit
CAGGAAANAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT4010.38423579237852495No Hit
CAGGAAANAGCTATGANNAAACTATTAATACCTGCAGCAAAGCCCCAAGC3950.3784866284027864No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA3910.3746538524189607No Hit
CAGGAAANAGCTATGANCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3800.3641137184634401No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3790.36315552446748367No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3600.34494983854431166No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3600.34494983854431166No Hit
CAGGAAANAGCTATGANCGGAAGGCAGGTGGTGGGAACTGGCCGTCGTTT3550.3401588685645296No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA3470.3324933165968782No Hit
CAGGAAANAGCTATGANCACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT3450.33057692860496535No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG3450.33057692860496535No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3390.32482776462922686No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC3330.3190786006534883No Hit
CAGGAAANAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT3290.3152458246696626No Hit
CAGGAAANAGCTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA3240.31045485468988054No Hit
CAGGAAANAGCTATGANNTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC3080.29512375075457775No Hit
CAGGAAANAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC3020.28937458677883926No Hit
CAGGAAANAGCTATGANNTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG3000.2874581987869264No Hit
CAGGAAACAGCTATGACCCCCGTGACAGCTCCTGCAATTACAAAATGGCT3000.2874581987869264No Hit
CAGGAAANAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC2890.2769180648314058No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATCTTCATGT2850.2730852888475801No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA2830.27116890085566725No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2820.2702107068597108No Hit
CAGGAAANAGCTATGANNAGGACTTGCCCACTCTCCCAGTTACTTGGAAT2690.25775418491227736No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2550.24433946896888745No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2540.24338127497293105No Hit
CAGGAAANAGCTATGANCCCCGTGACAGCTCCTGCAATTACAAAATGGCT2490.2385903049931489No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2490.2385903049931489No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC2480.2376321109971925No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2470.23667391700123605No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2440.2337993350133668No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2430.23284114101741038No Hit
CAGGAAANAGCTATGANCCATGGTTGGGGAAAAACTCCTCATCTTCATGT2380.22805017103762829No Hit
CAGGAAANAGCTATGANNCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG2360.22613378304571544No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG2310.2213428130659333No Hit
CAGGAAACAGCTATGACCCATGGTTGGGGAAAAACTCCTCATTTTCATGT2300.22038461906997692No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2270.21751003708210762No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC2160.206969903126587No Hit
CAGGAAANAGCTATGANNTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT2150.2060117091306306No Hit
CAGGAAANAGCTATGANNGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC2140.20505351513467415No Hit
CAGGAAANAGCTATGANCCATGGTTGGGGAAAAACTCCTCATTTTCATGT2090.20026254515489206No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2080.19930435115893563No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2060.19738796316702278No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2060.19738796316702278No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT2040.19547157517510994No Hit
CAGGAAANAGCTATGANNGCTGCTGACCCAACCCTACCCTGTCCAGCCCC2030.19451338117915354No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA2030.19451338117915354No Hit
CAGGAAACAGCTATGACCTTCATACAGAGATACAAAGGCAACTATGTGCA2000.19163879919128426No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2000.19163879919128426No Hit
CAGGAAANAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1990.19068060519532784No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1940.18588963521554575No Hit
CAGGAAACAGCTATGACCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1930.18493144121958932No Hit
CAGGAAANAGCTATGANCGCAAAACACCTTAACACTCGGCTTCTATTTGC1880.1801404712398072No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1870.1791822772438508No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1830.1753495012600251No Hit
CAGGAAANAGCTATGACCCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG1780.17055853128024298No Hit
CAGGAAANAGCTATGACCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1760.16864214328833016No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT1760.16864214328833016No Hit
CAGGAAANAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1740.1667257552964173No Hit
CAGGAAANAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1740.1667257552964173No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1700.1628929793125916No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1690.16193478531663522No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG1680.1609765913206788No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1630.1561856213408967No Hit
CAGGAAANAGCTATGANCAAACTATTAATACCTGCAGCAAAGCCCCAAGC1630.1561856213408967No Hit
CAGGAAANAGCTATGANCTTCATACAGAGATACAAAGGCAACTATGTGCA1600.15331103935302742No Hit
CAGGAAANAGCTATGANNGGAGAAGCACAGCTTCGGCTCTGGCCCTGGGG1580.15139465136111457No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1560.14947826336920172No Hit
CAGGAAANAGCTATGANCCTGGGTGCCGGGTAGAGTGTTGGGAAAGACTG1550.1485200693732453No Hit
CAGGAAANAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1520.14564548738537605No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA1490.14277090539750678No Hit
CAGGAAACAGCTATGACCCTGGGTGCCGGGCAGAGGGTTGGGAAAGACTG1460.1398963234096375No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1460.1398963234096375No Hit
CAGGAAANAGCTATGACCGCATCCTGCTTAGTGCTGCCTCAATCTGGTCC1450.13893812941368108No Hit
CAGGAAANAGCTATGANNCCCTGCGGCTCCTTTCAAGTTAAGTCACCACT1440.13797993541772466No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1410.1351053534298554No Hit
CAGGAAANAGCTATGANNAGGGCAAGTGTTCGCGTTGTAGGCGGCGGGAC1390.13318896543794256No Hit
CAGGAAANAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC1370.13127257744602971No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1360.1303143834500733No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1350.12935618945411687No Hit
CAGGAAANAGCTATGANNCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT1350.12935618945411687No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT1320.1264816074662476No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1310.1255234134702912No Hit
CAGGAAANAGCTATGACCCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1300.12456521947433478No Hit
CAGGAAACAGCTATGACCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1300.12456521947433478No Hit
CAGGAAANAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1300.12456521947433478No Hit
CAGGAAACAGCTATGACCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1270.12169063748646552No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT1260.1207324434905091No Hit
CAGGAAACAGCTATGANCGCATCAGATTCACTTCCACTGTCGTCAGAACT1250.11977424949455268No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT1250.11977424949455268No Hit
CAGGAAACAGCTATGACCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1240.11881605549859625No Hit
CAGGAAANAGCTATGANCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1240.11881605549859625No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGCC1190.11402508551881414No Hit
CAGGAAANAGCTATGANNAAGTTGGTCAGCCCTGGTGACTCATTGGATAT1170.1121086975269013No Hit
CAGGAAANAGCTATGANCTTTGTCGCCCTGGCTTGTCCCCAGACCTTCGG1170.1121086975269013No Hit
CAGGAAACAGCTATGACCAGGACTTGCCCACTCTCCCAGTTACTTGGAAT1170.1121086975269013No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1160.11115050353094488No Hit
CAGGAAANAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT1150.11019230953498846No Hit
CAGGAAANAGCTATGANCCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1130.10827592154307561No Hit
CAGGAAANAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC1110.10635953355116276No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA1110.10635953355116276No Hit
CAGGAAANAGCTATGANCTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1090.10444314555924993No Hit
CAGGAAACAGCTATGACCTCCAGTAAAACTGCCTCTGTTTCAACTCCCTT1080.1034849515632935No Hit
CAGGAAANAGCTATGANNTCCCACTTGCTCTTTGAAGGCAATTTCATTCT1070.10252675756733708No Hit
CAGGAAANAGCTATGANCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1070.10252675756733708No Hit
CAGGAAANAGCTATGANCTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1060.10156856357138065No Hit
CAGGAAANAGCTATGANCTCCCTCATTGTTGAGAGGCAGCTTAGTTTGTC1060.10156856357138065No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC1050.10061036957542424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)