FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67018
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT6110.9116953654242144No Hit
NNGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT4290.6401265331701931No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT3330.49688143483840164No Hit
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT3220.48046793398788384No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2980.444656659404936No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2930.4371959772001552No Hit
NAGGANNNNGNTATGNNNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT2780.41481393058581273No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2700.4028768390581635No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT2360.352144200065654No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2350.3506520636246978No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2320.34617565430182934No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2310.3446835178608732No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2230.33274642633322393No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT2130.3178250619236623No Hit
NNGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1880.2805216508997583No Hit
NNGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1870.27902951445880214No Hit
NAGGANNNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1860.27753737801784595No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1740.259631740726372No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1680.250678922080635No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1590.2372496941120296No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1420.21188337461577486No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT1410.21039123817481872No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAATT1380.20591482885195023No Hit
NAGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1370.20442269241099406No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1350.20143841952908176No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGGACACTGCCCAC1340.19994628308812556No Hit
NNGGANNNNGNTATGNNNTGGGCCTGGACACTTTGACGGCGGGGCACGTC1310.1954698737652571No Hit
NNGGANNNNGNTATGNNNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG1300.19397773732430093No Hit
NNGGANNNNGNTATGNNNCCGTAGCCACGCCCCTATTGTCTGACCACGCT1290.1924856008833448No Hit
NNGGANNNNGNTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1260.1880091915604763No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC1260.1880091915604763No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1240.18502491867856397No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1230.18353278223760783No Hit
NAGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1220.18204064579665163No Hit
NAGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT1210.1805485093556955No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1210.1805485093556955No Hit
NNGGANNNNGNTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1200.17905637291473933No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1170.17457996359187083No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC1110.16562714494613387No Hit
NAGGAANNNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1100.16413500850517773No Hit
NNGGANNNNGNTATGNNNCCCAAACCTGCGGAGGCACGGGGATGGCAGTG1100.16413500850517773No Hit
NNGGANNNNGNTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1080.1611507356232654No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1080.1611507356232654No Hit
NAGGANNNNGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1060.15816646274135307No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC1050.1566743263003969No Hit
NNGGAAANNGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT1000.1492136440956161No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1000.1492136440956161No Hit
NNGGANNNNGNTATGNNNCAGGGTGACTCCGATCCTGGAGCCAACGGTGC1000.1492136440956161No Hit
NNGGANNNNGNTATGNNNACAAACGCAAATGGCTTTTAGCATTTCACAGG990.14772150765465994No Hit
NAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT980.14622937121370377No Hit
NNGGANNNNGNTATGNNNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT980.14622937121370377No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT970.14473723477274764No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT960.14324509833179144No Hit
NNGGANNNNGNTATGNNNGGGAGGTGGAGGTTGCAGTGAGCCCAGACGGC950.1417529618908353No Hit
NNGGANNNNGNTATGANNTGGGCCTGGACACTTTGACGGCGGGGCACGTC950.1417529618908353No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG950.1417529618908353No Hit
NNGGANNNNGNTATGNNNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG930.13876868900892297No Hit
NAGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG920.1372765525679668No Hit
NNGGANNNNGNTATGNNNTGGAAACCCATGCCATGCACTCAACACTACAA920.1372765525679668No Hit
NNGGANNNNGNTATGANNCCGTAGCCACGCCCCTATTGTCTGACCACGCT910.13578441612701067No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC900.13429227968605448No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCCATACGGC890.13280014324509834No Hit
NNGGANNNNGNTATGANNCTCGTGCAGTCTGGTGTCCGACTTGCTGAGGG890.13280014324509834No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA880.13130800680414217No Hit
NNGGANNNNGNTATGANNCACGACACCCCACCTCTTGGGACACTGCCCAC860.12832373392222984No Hit
NAGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT850.1268315974812737No Hit
NAGGANNNAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT850.1268315974812737No Hit
NNGGANNNNGNTATGNNNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT850.1268315974812737No Hit
NNGGANNNNGNTATGNNNTGGCCCCAGTGTTGAGAGGTTCTTTTATTCCG820.12235518815840521No Hit
NAGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA810.12086305171744903No Hit
NNGGANNNNGNTATGNNNAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG810.12086305171744903No Hit
CAGGAAANAGCTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT800.11937091527649288No Hit
NAGGAAANAGNTATGANNTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT790.11787877883553673No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA780.11638664239458058No Hit
NNGGANNNNGNTATGNNNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT780.11638664239458058No Hit
NAGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT770.1148945059536244No Hit
NAGGANNNNGNTATGNNNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC760.11340236951266824No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGTGGAGACGGC760.11340236951266824No Hit
NNGGANNNNGNTATGNNNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA750.11191023307171209No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCGGCCTTGCTGA740.11041809663075591No Hit
NNGGANNNNGNTATGANNGGGAGGTGGAGGTTGCAGTGAGCCAAGACGGC740.11041809663075591No Hit
NAGGANNNNGNTATGNNNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA740.11041809663075591No Hit
NNGGANNNNGNTATGNNNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT740.11041809663075591No Hit
NNGGANNNNGNTATGANNACAAACGCAAATGGCTTTTAGCATTTCACAGG720.1074338237488436No Hit
NNGGANNNNGNTATGANNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT720.1074338237488436No Hit
NNGGANNNNGNTATGANNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA710.10594168730788743No Hit
NAGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT700.10444955086693128No Hit
NNGGANNNNGNTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA700.10444955086693128No Hit
NNGGANNNNGNTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT700.10444955086693128No Hit
NNGGANNNNGNTATGNNNCACGACACCCCACCTCTTGGACACTGCCCACC690.10295741442597511No Hit
NNGGANNNNGNTATGNNNACAGCTTTCTGAGGGTTGTTCCAGGTGCGGTG690.10295741442597511No Hit
NNGGANNNNGNTATGNNNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA690.10295741442597511No Hit
NNGGANNNNGNTATGANNAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG680.10146527798501895No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)