FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125429
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG29452.347941863524384No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGTGGTCC29002.3120649929442156No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA28292.2554592638066158No Hit
TGTAAAACGACGGCCAGTCAAGCAGACAGAGAGCTGAAGGAGTGCCAAGG23131.8440711478206795No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG22651.8058024858684993No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA21561.7189007326854238No Hit
TGTAAAACGACGGCCAGTACAAATGGAAATCCAGAGCAAGTCCTCCAGGG21051.6782402793612323No Hit
TGTAAAACGACGGCCAGTTCCCAAAGGCTGGTGGAGGAGATGCTGAAGAT19511.5554616555979877No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT16591.3226606287222253No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT15971.2732302737006593No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGCTGCTGAATG15821.261271316840603No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG15761.2564877340965805No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACTGTAAAACGAC14961.192706630842947No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCGCATTTCAGCA14931.1903148394709357No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCAGGGACAGCTGCC14541.1592215516347895No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG14261.1368981654960177No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGCCCCAATCCTGTCTC13761.0970349759624969No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA13531.078697908777077No Hit
TGTAAAACGACGGCCAGTGGCTCCCCAGCTGTCTCTCTTGTAAAACGACG13371.0659416881263504No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACACAAA12651.0085386951980801No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC12591.0037551124540576No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGTGGGAGCCCC12080.9630946591298662No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGACGGGTGGCC12040.9599056039671846No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC11690.93200137129372No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTGAGG11460.9136643041083004No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCAGCAGGATGGAG11290.9001108196669032No Hit
TGTAAAACGACGGCCAGTGGAGTGGCTGCCAGCAAGAGACAGTGTTCAGA11000.8769901697374609No Hit
TGTAAAACGACGGCCAGTCCATCCCACAGCCATCCGTGACTGTGACGACA10970.8745983783654497No Hit
TGTAAAACGACGGCCAGTGGTGGAGGCCCATTGAGGATCACATTTCAGCA10910.8698147956214273No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCACGAGGACATT10870.8666257404587455No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCGCCACGTGATCC10730.8554640473893597No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGTTCACT9700.7733458769503065No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGCTCCTTCCCAT9660.7701568217876249No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGCACGCAGGCT9600.7653732390436023No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACTGCCGAGTG9600.7653732390436023No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG9470.7550088097648869No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGTCATAGCTGT9190.7326854236261152No Hit
TGTAAAACGACGGCCAGTGGAGGATGCAAACCTGCCCACCACGTGCAGGA8520.6792687496511971No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCAGCCTGGGTGAT8380.6681070565818112No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC8350.6657152652098No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAACC8320.6633234738377888No Hit
TGTAAAACGACGGCCAGTCCTGCGCTATGGTTGGGGACTGTCCTCTCTCG8280.660134418675107No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCACAGTGACCTGT8120.6473781980243803No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCAGCAAA8090.6449864066523691No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG7960.6346219773736536No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC7880.6282438670482903No Hit
TGTAAAACGACGGCCAGTTGTGGACCACAGTTTGCAAGTGTAAAACGACG7630.6083122722815298No Hit
TGTAAAACGACGGCCAGTGGGGTAGTCAGCTGTCGTGTCGAGCAGGTGGC7410.5907724688867805No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC7340.5851916223520877No Hit
TGTAAAACGACGGCCAGTTCCAAGGTTCTGTATTTATTTATTGGGGAGAC6780.5405448500745442No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGCCTTCGGAGGC6700.5341667397491808No Hit
TGTAAAACGACGGCCAGTCCTGTGCCCCAGCCTCCTTAATGTGTGCACTC6520.5198159915171132No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCAGAGGTCAT6410.5110460898197386No Hit
TGTAAAACGACGGCCAGTTTGATGCAAGAAAAAGAAACTGGACTTAATGT6090.48553364851828523No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGCAAAGTCC6030.4807500657742627No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA6010.47915553819292184No Hit
TGTAAAACGACGGCCAGTTTGACCGTCCCACCTGCCTTGGTTTTCCCTTT5910.47118290028621773No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT5860.4671965813328656No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG5470.4361032934967193No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGGAGTGCAGTG5260.41936075389264044No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAACCGT5260.41936075389264044No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAG5040.4018209504978912No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAGGATCCCCGGTG4960.3954428401725279No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCTTCTGGGCTT4960.3954428401725279No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4730.37710577298710823No Hit
TGTAAAACGACGGCCAGTTGCCTTGAAGATTTTCTGTAGGATAATTTTGG4580.36514681612705197No Hit
TGTAAAACGACGGCCAGTCAGTTCCCAGGGACTCTGCTCTATGAGAGAGA4530.36116049717369986No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACGGCAGGAGA4510.35956596959235904No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAGACCCCGCCATG4460.35557965063900693No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCGGCTCACT4460.35557965063900693No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC4190.3340535282909056No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCAGGGCAGAAGGG4140.3300672093375535No Hit
TGTAAAACGACGGCCAGTGGCACCCTGACTACATCCTGGAGGAGACGAAC4030.3212973076401789No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGGGCTCAACCGT4030.3212973076401789No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGAAGCCTCAACCC3870.3085410869894522No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC3840.306149295617441No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGAGAGGCTCCTTCC3820.3045547680361001No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCTGCAGACAGGAT3790.30216297666408887No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC3700.29498760254805506No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGGGAGGGAGGAG3660.2917985473853734No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGGTGGGAC3660.2917985473853734No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3520.2806368543159875No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGCTGGGGCCGGA3500.2790423267346467No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCTCAGCTCACT3460.275853271571965No Hit
TGTAAAACGACGGCCAGTTGAAGAATGGTGGCTTGTTTCATGGTGGCCTT3430.27346148019995375No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG3390.2702724250372721No Hit
TGTAAAACGACGGCCAGTTGTTTACAAACGGTATGTTGCTGGTCATAGCT3320.26469157850257913No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA3240.2583134681772158No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGACCTGGCGCAGGC3140.2503408302705116No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCAGGCTGGAGTGC3090.24635451131715952No Hit
TGTAAAACGACGGCCAGTTTCTGCTCTTGGGCACAGGCTGGAGTGCAATG2970.2367873458291145No Hit
TGTAAAACGACGGCCAGTGGCTCTATATCAGGGCCCGCCGGTCATAGCTG2890.23040923550375114No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCTCATTAGCT2880.2296119717130807No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCCAAGAAACGTGA2860.22801744413173985No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCACACCACAGCC2850.22722018034106944No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCTTGGGAGAGTGC2830.2256256527597286No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGAGAGAAGGGGC2830.2256256527597286No Hit
TGTAAAACGACGGCCAGTTCTTTGGCTCTACCTAAAAGTTGGTCATAGCT2750.21924754243436523No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT2720.21685575106235402No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTGTAAAACGAC2720.21685575106235402No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAGAGCAAAGTC2710.21605848727168359No Hit
TGTAAAACGACGGCCAGTCCTGGGCGTCAGAGTGAGAACACCGCCGAGTG2660.2120721683183315No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTGGCCTGTTCG2660.2120721683183315No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG2640.21047764073699066No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCGTCACCAGGTGT2630.20968037694632022No Hit
TGTAAAACGACGGCCAGTGATCCAAGGCTCCTCGTCCGCAGCCTTTTTCC2630.20968037694632022No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC2590.20649132178363855No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGTCGGAGGTCAT2500.1993159476676048No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCCACTGGAGTGCA2460.19612689250492313No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTCACCATTTGGTG2400.19134330976090058No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCTGGGCAGAAGGG2390.19054604597023017No Hit
TGTAAAACGACGGCCAGTGGCAGGAGAGGCAGCTAACGGGGTTGGAGGTG2350.18735699080754847No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGCCTGGGTGGGA2330.18576246322620765No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCTACTTCAATGC2300.1833706718541964No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2250.1793843529008443No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGACGGTGCTCTGGT2240.17858708911017387No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGCAAGC2220.17699256152883303No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTAGAGCCTGGCT2210.17619529773816262No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCAGGATGGAGGG2190.1746007701568218No Hit
TGTAAAACGACGGCCAGTTGGTGTGGGCAGTGGTCCTAAGCCCCAGACCC2180.17380350636615138No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCAGGGCTTGAACCC2130.16981718741279928No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCCTAAGG2090.16662813225011758No Hit
TGTAAAACGACGGCCAGTGAGGACGAGAGCGAGGAGGAGACTTCCTGGGT2020.16104728571542465No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCGCTCCCACCCAG1930.1538719115993909No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGGCCCTCAACCCA1920.1530746478087205No Hit
TGTAAAACGACGGCCAGTTCTCTGGCTATTTCTTGGCATGATTCTCAGTG1900.15148012022737964No Hit
TGTAAAACGACGGCCAGTTCTTCACCTCCCAAAATGCTGGGATTACAGGC1880.1498855926460388No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGAAACC1880.1498855926460388No Hit
TGTAAAACGACGGCCAGTGGCACAGCCAAGAGTTGGGACCCACGAGGGCT1860.14829106506469794No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGTGACGCAGGTTC1850.14749380127402753No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCATAGCTCACT1770.1411156909486642No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT1770.1411156909486642No Hit
TGTAAAACGACGGCCAGTCCATTTCCGTCTGCCCCCTCTCTTGGGAGAGT1760.14031842715799375No Hit
TGTAAAACGACGGCCAGTCCCTGAAGGACAAGCCAGGCCCCCCAGGATGG1760.14031842715799375No Hit
TGTAAAACGACGGCCAGTGCTGGAGTGCTGTGGTGTGATCATAGCTTGGC1720.1371293719953121No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCATCATGTTCC1700.13553484441397126No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCATAAACGCAAA1690.13473758062330082No Hit
TGTAAAACGACGGCCAGTCGGTGCTCTGGTGAGATGGGTGGGGTCACCCC1680.1339403168326304No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG1680.1339403168326304No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCAGCTAACGGGGTT1680.1339403168326304No Hit
TGTAAAACGACGGCCAGTGTCCCAGGTGTATGGAGCGCTGCTGTGTGACC1570.1251704151352558No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGTGCACGGGAGC1510.12038683239123328No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCAGTTTCCTGCC1430.11400872206586993No Hit
TGTAAAACGACGGCCAGTACTGTCTGGAACCGCACTGCACTCCAGCCTGG1420.11321145827519952No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAAAGACCAGACG1360.10842787553117701No Hit
TGTAAAACGACGGCCAGTAGCCTTTCTACTCATTGGAACACTCATTTATT1340.10683334794983616No Hit
TGTAAAACGACGGCCAGTTCTGGTGTCGCCCATCACTGGCCCTCGTTCCC1320.10523882036849533No Hit
TGTAAAACGACGGCCAGTCACGCCATTCTCCTGCCTCAGCCTGGGCCTGG1310.1044415565778249No Hit
TGTAAAACGACGGCCAGTAAAATCAGGCCGGGCGTGGTGGCGGGCGCCTG1310.1044415565778249No Hit
TGTAAAACGACGGCCAGTGTGCAGTGGTGCAACCTTGGCTCACTGTGACC1300.10364429278715448No Hit
TGTAAAACGACGGCCAGTGGGAGGCCATGTTGAACTTATTCAGAATGTAC1290.10284702899648407No Hit
TGTAAAACGACGGCCAGTTCTCAGGAGAGCCCAAAGGGAGAGAGCAAAGT1260.1004552376244728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)