FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136151
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC15711.1538659282708168No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT13240.9724497065757872No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC12350.9070811084751489No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC12010.8821088350434445No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT11580.8505262539386417No Hit
CAGGAAANAGCTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC11530.8468538607869204No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC10570.7763439122738724No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG10570.7763439122738724No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG10350.760185382406299No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA10210.7499026815814794No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA9450.6940823056753164No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA9270.6808616903291199No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT8990.6602962886794809No Hit
CAGGAAANAGCTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA8940.6566238955277596No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT8750.6426688015512189No Hit
CAGGAAANAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC8490.6235723571622683No Hit
CAGGAAANAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT8070.5927242546878099No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT7740.5684864598864495No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA7610.5589382376919744No Hit
CAGGAAANAGCTATGANNAGGCACCAACAGTACAAAGCAGATACAAAGCA7230.5310280497388928No Hit
CAGGAAANAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC6900.5067902549375326No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG6870.5045868190464998No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC6800.49944546863409006No Hit
CAGGAAANAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG6730.49430411822168036No Hit
CAGGAAANAGCTATGANNTAGAGGACACCCAAGGCCACCAGGGCAGCGGT6580.48328693876651657No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG6460.47447319520238557No Hit
CAGGAAANAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC6350.4663939302685988No Hit
CAGGAAANAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC6230.4575801867044678No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT6170.4531733149224023No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG6110.44876644314033687No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA5860.4304044773817306No Hit
CAGGAAANAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC5850.42966999875138634No Hit
CAGGAAACAGCTATGACCACAGTGTCCCCATCAGGCTCCGGGACAGCATC5670.4164493834051898No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG5650.4149804261445013No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA5470.40175981079830486No Hit
CAGGAAANAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT5470.40175981079830486No Hit
CAGGAAANAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA5440.39955637490727214No Hit
CAGGAAANAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA5340.3922115886038296No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA5340.3922115886038296No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5130.3767875373666003No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5110.37531858010591185No Hit
CAGGAAACAGCTATGACCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4980.3657703579114366No Hit
CAGGAAANAGCTATGANCACAGGAGACAGGATTGGGGCAGCCCCGGCCCT4950.3635669220204038No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4860.3569566143473056No Hit
CAGGAAANAGCTATGANCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4680.34373599900110907No Hit
CAGGAAANAGCTATGANNGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC4630.3400636058493878No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4610.3385946485886993No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG4580.33639121269766653No Hit
CAGGAAACAGCTATGACCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4560.33492225543697807No Hit
CAGGAAANAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA4520.33198434091560103No Hit
CAGGAAANAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT4350.31949820419974884No Hit
CAGGAAACAGCTATGACCCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4320.31729476830871606No Hit
CAGGAAANAGCTATGANNAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC4280.3143568537873391No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC4270.3136223751569948No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC4130.3033396743321753No Hit
CAGGAAACAGCTATGACCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC4120.3026051957018311No Hit
CAGGAAANAGCTATGANCGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG4050.29746384528942127No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4020.29526040939838855No Hit
CAGGAAACAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3970.2915880162466673No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3890.2857121872039133No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3830.28130531542184783No Hit
CAGGAAANAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG3830.28130531542184783No Hit
CAGGAAANAGCTATGANCGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC3810.2798363581611593No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3810.2798363581611593No Hit
CAGGAAACAGCTATGACCCTGGAATTTCAACAGAATGGCATTTATATGAT3780.27763292227012654No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3730.2739605291184053No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3650.2680847000756513No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG3640.26735022144530707No Hit
CAGGAAANAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT3610.2651467855542743No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG3580.2629433496632415No Hit
CAGGAAACAGCTATGACCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3570.2622088710328973No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG3550.2607399137722088No Hit
CAGGAAACAGCTATGACCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3480.2555985633597991No Hit
CAGGAAANAGCTATGANNTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT3410.2504572129473893No Hit
CAGGAAANAGCTATGANCGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC3410.2504572129473893No Hit
CAGGAAANAGCTATGANCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3400.24972273431704503No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG3390.2489882556867008No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA3370.24751929842601228No Hit
CAGGAAANAGCTATGANCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3240.23797107623153702No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3220.23650211897084855No Hit
CAGGAAANAGCTATGANCCAGCTGTGTCTCCAGCTCCAGAACCTGCACCA3190.23429868307981577No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTGGCCGTC3170.2328297258191273No Hit
CAGGAAANAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3120.22915733266740604No Hit
CAGGAAANAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3090.22695389677637332No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG3080.22621941814602903No Hit
CAGGAAACAGCTATGACCCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA3070.2254849395156848No Hit
CAGGAAANAGCTATGANNAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT3050.22401598225499628No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2950.21667119595155376No Hit
CAGGAAANAGCTATGANNGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG2890.2122643241694883No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2880.21152984553914406No Hit
CAGGAAANAGCTATGANCCTGGAATTTCAACAGAATGGCATTTATATGAT2860.21006088827845554No Hit
CAGGAAACAGCTATGACCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2800.20565401649639006No Hit
CAGGAAANAGCTATGANNTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC2800.20565401649639006No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA2760.20271610197501302No Hit
CAGGAAANAGCTATGANCAGGCACCAACAGTACAAAGCAGATACAAAGCA2760.20271610197501302No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG2730.2005126660839803No Hit
CAGGAAANAGCTATGANNCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2710.19904370882329178No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2590.19022996525916078No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA2560.18802652936812803No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGGAACACACAACTGGC2560.18802652936812803No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2490.1828851789557183No Hit
CAGGAAANAGCTATGANCCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT2480.18215070032537403No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2450.17994726443434128No Hit
CAGGAAANAGCTATGACCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC2430.1784783071736528No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2400.17627487128262004No Hit
CAGGAAANAGCTATGANNGCCCCTACTCCAAAAGGCGGATGAATGGGGAA2400.17627487128262004No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2390.17554039265227578No Hit
CAGGAAANAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC2370.17407143539158726No Hit
CAGGAAACAGCTATGACCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2300.16893008497917752No Hit
CAGGAAANAGCTATGANCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2280.16746112771848903No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2280.16746112771848903No Hit
CAGGAAANAGCTATGANCTAGAGGACACCCAAGGCCACCAGGGCAGCGGT2270.16672664908814477No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG2260.16599217045780051No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2250.16525769182745628No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2190.16085082004539078No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2190.16085082004539078No Hit
CAGGAAANAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT2180.16011634141504652No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG2110.15497499100263679No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2080.152771555111604No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCTAAACCAG2040.14983364059022702No Hit
CAGGAAANAGCTATGANCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2040.14983364059022702No Hit
CAGGAAANAGCTATGANNGGGTCCCACCCATGCTGAAAGTCCACCTTCCA2040.14983364059022702No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2040.14983364059022702No Hit
CAGGAAACAGCTATGACCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2030.1490991619598828No Hit
CAGGAAANAGCTATGANCTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA2020.14836468332953853No Hit
CAGGAAANAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2010.14763020469919427No Hit
CAGGAAANAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2000.14689572606885No Hit
CAGGAAACAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1980.14542676880816152No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC1930.14175437565644028No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1900.13955093976540753No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGGAACACACAACTGGC1900.13955093976540753No Hit
CAGGAAANAGCTATGANCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1900.13955093976540753No Hit
CAGGAAANAGCTATGANNCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1890.13881646113506327No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC1850.13587854661368629No Hit
CAGGAAANAGCTATGACCTTCATACAGAGATACAAAGGCAACTGGCCGTC1840.13514406798334203No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1840.13514406798334203No Hit
CAGGAAANAGCTATGANNTTCATACAGAGATACAAAGGCAACTATGTGCA1810.13294063209230927No Hit
CAGGAAANAGCTATGACCGAGGCGGAAGTGGTGCTCATCCACGTACTGTG1800.13220615346196501No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAGG1780.13073719620127652No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1770.1300027175709323No Hit
CAGGAAANAGCTATGACCCTGTGAGGCACCAGAGCACCGCGGAGAGTAAG1770.1300027175709323No Hit
CAGGAAANAGCTATGANCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1760.12926823894058803No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1740.12779928167989954No Hit
CAGGAAANAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT1730.12706480304955528No Hit
CAGGAAANAGCTATGACCGCTCCTGTGCTGTCAAGGGACTCCTGTAGTGG1730.12706480304955528No Hit
CAGGAAANAGCTATGACCTCCTGGAACCTGCCTCCGAAAAGGCCGTGCGC1710.12559584578886676No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1670.12265793126748976No Hit
CAGGAAANAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT1660.12192345263714552No Hit
CAGGAAACAGCTATGACCGTCTCAGGGATCACGTGGCGCCGCAGCCAGCC1660.12192345263714552No Hit
CAGGAAANAGCTATGACCTGGGGATGAGGACAGCAGGGCAGTCACCATCT1650.12118897400680129No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1580.11604762359439151No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1560.11457866633370302No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTCACCATCCAACT1540.11310970907301451No Hit
CAGGAAACAGCTATGACCCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1510.11090627318198178No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC1470.10796835866060477No Hit
CAGGAAACAGCTATGACCTGCTGGGAGAGGGGGATGAGCGGCGCCGCCAT1430.10503044413922777No Hit
CAGGAAANAGCTATGANCAGATCCTGTGAAGTGGGAGGGTTTTCAAAGTC1430.10503044413922777No Hit
CAGGAAANAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG1390.10209252961785077No Hit
CAGGAAANAGCTATGANCCTGTGAGGCACCAGAGCACCGACTGGCCGTCG1380.10135805098750651No Hit
CAGGAAANAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1380.10135805098750651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)