FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71353
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA30434.264712065365156No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG22943.2150014715569073No Hit
TGTAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA16552.3194539823132874No Hit
TGTAAAACGACGGCCAGTGTGTTTATCGCGGCTAGAGAGATGTCGCTGCT14121.9788936695023334No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG12001.6817793225232296No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG11841.65935559822292No Hit
TGTAAAACGACGGCCAGTGCAGGTGCAGCTGCCACAGTGAGACGGGCACC10711.5009880453519824No Hit
TGTAAAACGACGGCCAGTGGTGGTGATGGGCAGACAACAGACGGCACTGA10381.4547391139825936No Hit
TGTAAAACGACGGCCAGTCAGCCAGTAGCAACAGCAGCTACTGATGTAAG10301.4435272518324387No Hit
TGTAAAACGACGGCCAGTGCTAGACATCGCCTGGTGCCCGCACAATGACA9901.3874679410816644No Hit
TGTAAAACGACGGCCAGTACCTGAATGCTCGCAGCTCCCGGGACTTTGAG9631.3496279063248917No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT8211.150617353159643No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG8121.1380040082407186No Hit
TGTAAAACGACGGCCAGTCCCAGCACAACTTCCCCCATGGCGTGGTGTCC8031.1253906633217945No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGATCCTGG7941.1127773184028702No Hit
TGTAAAACGACGGCCAGTGGGCCCCTGAAGAAGTCCAATGCACCGCTTGT7731.0833461802587137No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGCAGGAGACAGGC7080.9922498002887056No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC6990.9796364553697812No Hit
TGTAAAACGACGGCCAGTTGTGTGTTCCTGCTCCTGGCCATTGTGGGCTA6840.9586142138382409No Hit
TGTAAAACGACGGCCAGTGTGGTGCTGGCACTGTCTGGGCCAGAGCCTGG6820.9558112483007022No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC6280.8801311787871567No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCATAGCTGTT5990.8394881784928454No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGTGTAAAACGACG5980.8380866957240761No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC5790.8114585231174584No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT5540.7764214538982244No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGTGGCTACGGGGT4620.6474850391714434No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCTCAGCCTTCTGGGTC4580.641879108096366No Hit
TGTAAAACGACGGCCAGTCCAGAGATCAATAATAGCTCAGGGGAGATGGA4170.5844183145768224No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC3930.5507827281263576No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCAGGGGTGCTGGAGGC3830.5367679004386642No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT3170.44427003769988643No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTGGCTGAGGAGCTCAAGGGT3050.4274522444746542No Hit
TGTAAAACGACGGCCAGTGAGGAGCGGGTGCTCTCGGGGGTGCTGGAGGC2960.41483889955573No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC2920.40923296848065255No Hit
TGTAAAACGACGGCCAGTCACTGTGGAGGAGGTGGGGAGGTGTTGGGCCA2900.4064300029431138No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC2880.40362703740557504No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGCCCCTTGGGGGA2840.3980211063304977No Hit
TGTAAAACGACGGCCAGTGTTCTACCGTCAGTCCTGGCATTATTTCTCTC2770.3882107269491122No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACGAATCCCGGTCGGGTTCTGG2710.379801830336496No Hit
TGTAAAACGACGGCCAGTTCCATTACTCTACACAAGCAGGGTGAAGGTGA2660.3727944164926492No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCGTGACAAGGCT2470.34616624388603146No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGCACGATATCCAT2310.3237425195857217No Hit
TGTAAAACGACGGCCAGTGCATGAGGCGAATTCTCAGCCATGACAAGGCT2300.32234103681695236No Hit
TGTAAAACGACGGCCAGTGAGGGAGGCCAAGGGTGTTGGTCAGCACCTCT2300.32234103681695236No Hit
TGTAAAACGACGGCCAGTAGATCATCCAGCGCACACGGTGTAAAACGACG2290.320939554048183No Hit
TGTAAAACGACGGCCAGTGCCCAAGGTTGGTGAGATGGGGCCAGGTCATA2220.31112917466679746No Hit
TGTAAAACGACGGCCAGTCAGCCCTGTCACTTCCTGCTCCATCCCCGGTC2170.30412176082295067No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCATGGGTC2170.30412176082295067No Hit
TGTAAAACGACGGCCAGTAGTGACAACAGCCCCAAAGGAATGATCCCGCT2140.2999173125166426No Hit
TGTAAAACGACGGCCAGTCACCTTCCCTCTGCTGCTGGGGACCCTCCAAC2100.2943113814415652No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2040.28590248482894903No Hit
TGTAAAACGACGGCCAGTGAGCATGTTTGCGGTGGGCTGCAGCGTGGGTC1850.2592743122223312No Hit
TGTAAAACGACGGCCAGTGCAACTGCGCCTTACACAGTACCATGGAGGAT1820.25506986391602315No Hit
TGTAAAACGACGGCCAGTTCTATTGCTGAGACTTTCCAGAGCACTGGTGG1630.22844169130940536No Hit
TGTAAAACGACGGCCAGTAAACCTGGCCCCACTGCCTCCTGCTCAGCCTT1570.22003279469678919No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT1570.22003279469678919No Hit
TGTAAAACGACGGCCAGTCGGCTCTGGCTTAAGGGGTGGCTACGGGGTCA1510.21162389808417306No Hit
TGTAAAACGACGGCCAGTTCATAGCTGTGAAGAGGGATACCAAGGAAAGT1500.2102224153154037No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGGGGCCTCTGGG1470.20601796700909564No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGGAGCTGCACCG1320.18499572547755527No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCAGGTCATA1200.16817793225232294No Hit
TGNAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAATAAGGAGACGATG1070.1499586562583213No Hit
TGTAAAACGACGGCCAGTTCACCATGTTGGCCAGGCTGGTCTCGAACTCC1010.14154975964570515No Hit
TGTAAAACGACGGCTAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG1000.1401482768769358No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC980.1373453113393971No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCCATGGTTCG960.13454234580185837No Hit
TGTAAAACGACGGCCAGTTCCCAGCTAGCTGCAGAGGTCCCTGGTGTCCA930.13033789749555028No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG920.12893641472678094No Hit
TGTAAAACGACGGCCAGTCCTGCCGCCTGGTCTCACCTCGCTATGGTTCG910.12753493195801158No Hit
TGNAAAACGACGGCCAGTAGCACGAAAAGTAGGTGGCTTTACTGGTAGCA900.1261334491892422No Hit
TGTAAAACGACGGCCAGTCTGCTGATGGGGAAGTCCGAAGGCCCCGTGGG900.1261334491892422No Hit
TGNAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAATCTCCACGCAGGA850.11912603534539543No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC840.11772455257662608No Hit
TGTAAAACGACGGCTAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA830.11632306980785671No Hit
TGTAAAACGACGGCTAGTAGCACGAAAAGTAGGTGGCCTTACTGGTAGCA820.11492158703908736No Hit
TGTAAAACGACGGCCAGTGCCGCGGCGCTGTAGTGCGGCGCCCCAGGTTC780.10931565596400993No Hit
TGTAAAACGACGGCCAGTTGACACAGCTGCTTCTGAGTACAAGTATGTAG770.10791417319524056No Hit
TGTAAAACGACGGCCAGTCGCCTGAATAGGTGCAGGGGTACAGTCACAGC760.10651269042647121No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCTCAACAAGGAGACGATG750.10511120765770185No Hit
TGTAAAACGACGGCCAGTGCAGAACACCTCCTCTTGACTGCTCTAAGCAC720.10090675935139376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)