FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71353
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG13371.8737824618446315No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT10231.4337168724510532No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT10111.416899079225821No Hit
CAGGAAANAGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA6990.9796364553697812No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC6920.9698260759883958No Hit
CAGGAAANNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT5360.7511947640603759No Hit
CAGGAAANNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT5020.7035443499222177No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC4810.6741132117780612No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC4470.626462797639903No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT4380.6138494527209788No Hit
CAGGAAANAGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT4250.5956301767269772No Hit
CAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG4250.5956301767269772No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT4010.5619945902765126No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT3840.5381693832074335No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT3600.5045337967569689No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT3560.4989278656818914No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC3470.4863145207629672No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT3400.4765041413815817No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC3300.46248931369388807No Hit
CAGGAAANAGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT3150.44146707216234776No Hit
CAGGAAANNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3000.4204448306308074No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGACAGGGCTGACTG3000.4204448306308074No Hit
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2900.4064300029431138No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2890.4050285201743445No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2710.379801830336496No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2610.36578700264880243No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA2560.35877958880495564No Hit
CAGGAAANAGCTATGANNCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT2540.3559766232674169No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2270.3181365885106443No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC2240.3139321402043362No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2150.30131879528541194No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT2090.2929098986727958No Hit
CAGGAAANAGCTATGANNAAGGACAAGGTGAGGCAGCACTGAACTGACAT2050.28730396759771837No Hit
CAGGAAANAGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT2040.28590248482894903No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAATT2010.28169803652264097No Hit
CAGGAAANNGCTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1990.27889507098510224No Hit
CAGGAAANAGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT1980.2774935882163329No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA1970.2760921054475635No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1740.24385800176586828No Hit
CAGGAAANNGCTATGANNACCTCTCTGGGCCTGTCTCCTGCCGTGTGCGC1730.24245651899709894No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1710.23965355345956021No Hit
CAGGAAANAGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA1710.23965355345956021No Hit
CAGGAAANAGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC1680.23544910515325215No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1640.22984317407817473No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGACAGGAAACAGCT1620.22704020854063597No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA1590.2228357602343279No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1550.2172298291592505No Hit
CAGGAAANAGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1550.2172298291592505No Hit
CAGGAAANNGCTATGANNGGGGATGGAGCAGGAAGTGACAGGGCTGACTG1530.21442686362171176No Hit
CAGGAAANNGCTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1490.20882093254663434No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1460.20461648424032627No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1440.20181351870278752No Hit
CAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1420.19901055316524885No Hit
NAGGAAANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1350.18920017378386333No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG1320.18499572547755527No Hit
CAGGAAANAGCTATGANNAGAGACCAGATGCATAAGCACAGCAGCATTCC1320.18499572547755527No Hit
CAGGAAANNGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1300.18219275994001655No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1280.17938979440247782No Hit
CAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1280.17938979440247782No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT1270.17798831163370846No Hit
CAGGAAACNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1230.17238238055863103No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1190.1667764494835536No Hit
CAGGAAANNGCTATGANNCAGAAGGCTGAGCAGGAGGCCAGGAAACAGCT1180.16537496671478424No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1170.16397348394601488No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG1160.16257200117724552No Hit
CAGGAAACNGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1150.16117051840847618No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA1140.1597690356397068No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1090.15276162179586003No Hit
CAGGAAANAGCTATGANNTGGCCCCAGTGTTGAGAGGTCAGGAAACAGCT1090.15276162179586003No Hit
CAGGAAANAGCTATGANNAACACGGCAGTCAGAGGTGCTGACCAACACCC1090.15276162179586003No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1080.15136013902709067No Hit
CAGGAAANAGCTATGANNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT1040.14575420795201321No Hit
NAGGANANNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1040.14575420795201321No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1030.14435272518324388No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCACTTGAGCCTG1020.14295124241447452No Hit
CAGGAAANAGCTATGANNGGGGATGGAGCAGGAAGTGAGTCCTCCACCGC1000.1401482768769358No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCATGCACGCGCA980.1373453113393971No Hit
CAGGAAANNGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA970.1359438285706277No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT960.13454234580185837No Hit
CAGGAAANAGCTATGANNTTCCCTGTGCCTGGCCCTATAGATGTTGACAT900.1261334491892422No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC900.1261334491892422No Hit
CAGGAAANNGCTATGANNAAGGACAAGGTGAGGCAGCACTGAACTGACAT890.12473196642047285No Hit
CAGGAAANNGCTATGANNGCCCAGCAATGGAGAGGCTACCAGCTGATCCA880.1233304836517035No Hit
CAGGAAANNGCTATGANNTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC840.11772455257662608No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA840.11772455257662608No Hit
CAGGAANNNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG830.11632306980785671No Hit
CAGGAANNNGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT830.11632306980785671No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT830.11632306980785671No Hit
CAGGAAANAGCTATGANNGGCTGGCACAAAGAACAGCACTGACTGTTTAT820.11492158703908736No Hit
CAGGAAANNGCTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT780.10931565596400993No Hit
CAGGAAACNGCTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA780.10931565596400993No Hit
CAGGAAANNGCTATGANNCATGGTTGGGGAAAAACTCCTCATTTTCATGT770.10791417319524056No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA770.10791417319524056No Hit
CAGGAAANAGCTATGANNACAAGACGCTTCAACACCCCCACCAGTGCTCT770.10791417319524056No Hit
CAGGAAANAGCTATGANNACCTCTCTGGGCCTGTCTCCTGGCCGTCGTTT760.10651269042647121No Hit
CAGGAAACNGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG740.1037097248889325No Hit
CAGGAAANNGCTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA740.1037097248889325No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC730.10230824212016314No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT720.10090675935139376No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG720.10090675935139376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)