FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129656
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAAANGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG40133.095113222681557No Hit
TGTAAAACGACGGCCAGTAGCAGCTTGTCTTCCCCAACAAGGAGACGATG38852.996390448571605No Hit
TGTAAAANGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA36682.829024495588326No Hit
TGTAAAACGACGGCCAGTCGCAGGTGTGTGAAGAAAACCTCCACGCAGGA34372.650860739186771No Hit
TGTAAAANGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT25611.9752267538717838No Hit
TGTAAAACGACGGCCAGTACTCCCTCGCTAAGTGCCTCGGCAAGGAGGAT22051.7006540383784785No Hit
TGTAAAANGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA19961.5394582587770715No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG18521.428395137903375No Hit
TGTAAAANGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC18151.399858086012217No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGGGTAGCCACAG18141.399086814339483No Hit
TGTAAAANGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT17751.3690072191028568No Hit
TGTAAAANGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG17351.3381563521934967No Hit
TGTAAAACGACGGCCAGTGCCAGTGTCTGAATTGCTGAGGAGATGTGGGA17291.3335287221570926No Hit
TGTAAAACGACGGCCAGTCAGGAGCACGTGGAGAGGCCGAGTAGCCACAG16501.2725982600111063No Hit
TGTAAAACGACGGCCAGTCCTGGAACTGCATCATCAGGATCTTCACGATT16471.2702844449929043No Hit
TGTAAAACGACGGCCAGTCAGCGAGCGCACAAGCGCTTCCAGACCTACGC16031.2363484913926082No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT14971.1545936940828037No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC14371.1083173937187634No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGTTGCTGAT13301.0257913247362251No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTACGGGGCGCCAC13231.020392423027087No Hit
TGTAAAANGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG12070.9309249089899427No Hit
TGTAAAANGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG12020.9270685506262725No Hit
TGTAAAANGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC11380.8777071635712964No Hit
TGTAAAACGACGGCCAGTAGGTCATCCTCCTGCCTCGGCCTCCCAAAGTG10950.8445424816437341No Hit
TGTAAAACGACGGCCAGTACACATTGAGAGCATCAGTGGGGGGCAATCAG10830.8352872215709262No Hit
TGTAAAACGACGGCCAGTCAGGACCAAGCAGACACCCACAACCAGCAGCC9600.7404208058246436No Hit
TGTAAAANGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG9290.7165113839698896No Hit
TGTAAAACGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA9060.6987721354970075No Hit
TGTAAAANGACGGCCAGTCGGGTGGGTCTGGGTCTCTTGCTGCGGTCATA8920.6879743320787314No Hit
TGTAAAANGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC8910.6872030604059974No Hit
TGTAAAANGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC8520.6571234651693713No Hit
TGTAAAACGACGGCCAGTTCCTGGGCTTCTCTTGAGTACGGAGGAAAGTG8470.6532671068057012No Hit
TGTAAAANGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC8030.6193311532054051No Hit
TGTAAAACGACGGCCAGTCTCTGGCTCAGCGGACACGCGGGGACTCAGGC7850.605448263096193No Hit
TGTAAAANGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC7580.5846239279323748No Hit
TGTAAAACGACGGCCAGTATCCAGAGCCCAGAGCAGGTCATAGCTGTTTC7540.5815388412414388No Hit
TGTAAAANGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT7540.5815388412414388No Hit
TGTAAAACGACGGCCAGTGGGATCAGGAGAACGTACACCGTTGAGTATAC7340.5661134077867588No Hit
TGTAAAACGACGGCCAGTTGGGCCTCTTCAGCATAGTCCTGTGCCAGGGC6950.5360338125501326No Hit
TGTAAAANGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6720.5182945640772506No Hit
TGTAAAACGACGGCCAGTCCAGCCCTCCCTTGCACGTTCCGGCTCCTCTT6520.5028691306225704No Hit
TGTAAAACGACGGCCAGTTACAGGCCCCACACTGCTGACTCTGCTCCCCT6040.4658480903313383No Hit
TGTAAAANGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA5970.46044918862220025No Hit
TGTAAAACGACGGCCAGTCCCCCAGAAAATTGTGCCAAAGAGTTTAGAAA5930.45736410193126426No Hit
TGTAAAANGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG5670.43731103844018016No Hit
TGTAAAANGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT5500.42419942000370214No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT5440.4195717899672981No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGTCATAGCTGTTT5200.40106126982168194No Hit
TGTAAAACGACGGCCAGTGCAAGCCTGCCTCCAATTCCATTCAAAGTCAT4970.3833220213487999No Hit
TGTAAAACGACGGCCAGTCCCCACACACAAAGCCTGGTCATAGCTGTTTC4870.37560930462145986No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG4830.3725242179305238No Hit
TGTAAAANGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC4820.3717529462577899No Hit
TGTAAAANGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG4770.36789658789411983No Hit
TGTAAAANGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG4720.3640402295304498No Hit
TGTAAAACGACGGCCAGTGGGTAGGAAGTCGGGAGGCACTGCCTAGCAGG4700.3624976861849818No Hit
TGTAAAANGACGGCCAGTCCCCACACACAAAGCCTGGTCATAGCTGTTTC4600.35478496945764176No Hit
TGTAAAANGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG4600.35478496945764176No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGGAGGAGTGGTCATAG4580.35324242611217377No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC4560.3516998827667058No Hit
TGTAAAANGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4520.34861479607576973No Hit
TGTAAAANGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA4500.34707225273030173No Hit
TGTAAAANGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT4450.34321589436663175No Hit
TGTAAAACGACGGCCAGTTGAAGTTGTGGGGAGCTGCGGTCATAGCTGTT4390.33858826433022765No Hit
TGTAAAACGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT4280.3301042759301536No Hit
TGTAAAANGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG4220.32547664589374964No Hit
TGTAAAANGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4200.3239341025482816No Hit
TGTAAAACGACGGCCAGTCTGGGCCTTGGCTTCTTCCTCAATCATCTAGG4100.31622138582094156No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCACACTGCTGC4090.31545011414820756No Hit
TGTAAAACGACGGCCAGTGGGGAGAGGAAGGCCAGTGATATCTCCTGCAC4000.30850866909360153No Hit
TGTAAAACGACGGCCAGTCCCTGCCTGTGAGCGTGTGGCGCCCGCTTTCC3990.30773739742086753No Hit
TGTAAAACGACGGCCAGTATCTGCCGGTCTGCCCTCTGCCGCACTGCTGC3920.3023384957117295No Hit
TGTAAAACGACGGCCAGTCTGCAAAGCGAGCGCGAGTGTCGACGCGTGCG3900.3007959523662615No Hit
TGTAAAANGACGGCCAGTCCAGAGCCCAGCATGGAAGGGTCATAGCTGTT3850.2969395940025915No Hit
TGTAAAACGACGGCCAGTTCCCACCACCATACCCCAACTTAGTATTTGAA3820.29462577898438946No Hit
TGTAAAACGACGGCCAGTCGCAATGTGGCCTGGTTCTGCGTGTCTGCTGG3760.2899981489479854No Hit
TGTAAAACGACGGCCAGTACCTCATGTCACGTGCGTCCATGTGAAGAGAG3750.2892268772752514No Hit
TGTAAAANGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3740.28845560560251743No Hit
TGTAAAANGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT3470.26763127043869933No Hit
TGTAAAANGACGGCCAGTACCGACTGGAGAGGAAGGCCAGGAGGAGTGGT3430.26454618374776334No Hit
TGTAAAACGACGGCCAGTACCGACTGGAGAGGAAGGCCAGGAGGAGTGGT3280.25297710865675327No Hit
TGTAAAACGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCGGTCATAGCTG3270.25220583698401927No Hit
TGTAAAACGACGGCCAGTGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAG3140.2421793052384772No Hit
TGTAAAANGACGGCCAGTAAGAGGTGAGCGAGGTCTGCCGGTCATAGCTG3040.23446658851113714No Hit
TGTAAAANGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2900.22366878509286112No Hit
TGTAAAACGACGGCCAGTCTTTCCCAACCCTCTGCCCGGCCGGTGCCCAT2800.21595606836552106No Hit
TGTAAAANGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT2790.2151847966927871No Hit
TGTAAAACGACGGCCAGTAGTACTCCCTCCTGCCTGTGGTCATAGCTGTT2680.20670080829271303No Hit
TGTAAAANGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA2640.203615721601777No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2630.20284444992904302No Hit
TGTAAAACGACGGCCAGTGCCATCGGCTCCCAAGTTTTATGTCCTAGAGC2600.20053063491084097No Hit
TGTAAAACGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGAGGCTGTCTG2510.19358918985623497No Hit
TGTAAAANGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT2420.18664774480162893No Hit
TGTAAAANGACGGCCAGTTGTCTGGTGGTGGAAGGAGGAGAGGCTGTCTG2360.1820201147652249No Hit
TGTAAAANGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2360.1820201147652249No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCTCGCTGTCCGCACTGCG2350.1812488430924909No Hit
TGTAAAACGACGGCCAGTAGCACAGCTGCCCTTGGATCGACATGGAGAAA2260.17430739803788486No Hit
TGTAAAANGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC2170.16736595298327883No Hit
TGTAAAACGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT2140.1650521379650768No Hit
TGTAAAACGACGGCCAGTCAAGGTCTTGTATAGGGAGATACAATGATGTA2100.1619670512741408No Hit
TGTAAAACGACGGCCAGTGGTACGTGGTGGTAGCTATTTGGGTAAAGGTG2070.1596532362559388No Hit
TGTAAAACGACGGCCAGTGTGGTAACTGCTGCAGGGCTTAATACATTAGT2070.1596532362559388No Hit
TGTAAAANGACGGCCAGTGGTACGTGGTGGTAGCTATTTGGGTAAAGGTG2040.15733942123773678No Hit
TGTAAAANGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1990.15348306287406677No Hit
TGTAAAACGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1970.15194051952859877No Hit
TGTAAAANGACGGCCAGTTGGAGTGTCTAGGTTTGAAAGCTGCTGGTCAT1960.15116924785586475No Hit
TGTAAAANGACGGCCAGTCTGCCACCAGCACCCAGGCTGCGGGGGTACCG1930.14885543283766273No Hit
TGTAAAANGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT1900.14654161781946073No Hit
TGTAAAACGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1840.1419139877830567No Hit
TGTAAAANGACGGCCAGTTGGGGTCTCCTGGTCCCATTCGAGCATGCTGC1760.1357438144011847No Hit
TGTAAAANGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG1730.13342999938298267No Hit
TGTAAAACGACGGCCAGTTGGAGTGCCTGGGTTAGAACCCTGCTGGTACC1720.13265872771024864No Hit
TGTAAAACGACGGCCAGTACGTCAGCAGGCGGCGCGCTGTCCGCACTGCG1710.13188745603751464No Hit
TGTAAAACGACGGCCAGTTGAGGAGCCTGGAACAGGGCTCTGAATTGTCT1690.13034491269204665No Hit
TGTAAAACGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAACAGGAAAA1690.13034491269204665No Hit
TGTAAAACGACGGCCAGTAGGAGGAGAGGCTGTCTGCCGGTCATAGCTGT1690.13034491269204665No Hit
TGTAAAANGACGGCCAGTAGTGTGGGTTGTTTGAAAGCCAAACAGGAAAA1680.12957364101931265No Hit
TGTAAAANGACGGCCAGTAGGAGGAGAGGCTGTCTGCCGGTCATAGCTGT1630.1257172826556426No Hit
TGTAAAACGACGGCCAGTTGGAACCATTTTTGATAACTTTCTGATCACAG1610.12417473931017461No Hit
TGTAAAANGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCTCAAACCC1590.1226321959647066No Hit
TGTAAAACGACGGCCAGTTCCATCCACTGCTGAGAAATCTCCTCAAACCC1540.1187758376010366No Hit
TGTAAAACGACGGCCAGTGGCTGACGCAGGAGAATTGCTTGAACCTGGGA1420.10952057752822854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)