FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129656
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG25761.9867958289627938No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT24321.875732708089097No Hit
CAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT22881.764669587215401No Hit
CAGGAAANAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT16231.2517739248472883No Hit
CAGGAAANAGCTATGANNACCAAGACCCCAGCAGCATTAAAAAGGCTGCT15761.2155241562287902No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT14861.1461097056827296No Hit
CAGGAAANAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG13961.0766952551366693No Hit
CAGGAAANAGCTATGANNTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA10880.8391435799345962No Hit
CAGGAAANAGCTATGANNTGACCATCTCTCCTAGACGCCAGTGCAGCTGC10490.80906398469797No Hit
CAGGAAACAGCTATGACCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT10430.8044363546615659No Hit
CAGGAAACAGCTATGACCTGTTTTGCCCCTGAAATGAATATTCATCGTCT9990.7705004010612698No Hit
CAGGAAANAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT9990.7705004010612698No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG9220.7111124822607515No Hit
CAGGAAANAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG9200.7095699389152835No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA9140.7049423088788795No Hit
CAGGAAANAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT8830.6810328870241255No Hit
CAGGAAANAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT8640.6663787252421792No Hit
CAGGAAANAGCTATGANNCATGTGCCCCAGTAGAGAGCAATTAAATTCCT7720.5954217313506509No Hit
CAGGAAANAGCTATGANNTGACAGCACAAGGAACACTGACCTACAGATAA7200.5553156043684827No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT6720.5182945640772506No Hit
CAGGAAANAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA6530.5036404022953045No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCCAGACCCACCCGACTGGCC6490.5005553156043685No Hit
CAGGAAANAGCTATGANNCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC6330.48821496884062443No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTTCATCACAAAA5960.45967791694946625No Hit
CAGGAAACAGCTATGACCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5910.45582155858579626No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT5870.45273647189486027No Hit
CAGGAAANAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC5800.44733757018572223No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA5620.43345468007651017No Hit
CAGGAAANAGCTATGANNCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT5500.42419942000370214No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG5390.415715431603628No Hit
CAGGAAANAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT5350.412630344912692No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA5240.404146356512618No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG5200.40106126982168194No Hit
CAGGAAANAGCTATGANNTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT5120.39489109643980996No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA5110.39411982476707597No Hit
CAGGAAANAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA5000.38563583636700194No Hit
CAGGAAANAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT4690.3617264145122478No Hit
CAGGAAANAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA4270.3293330042574196No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA4070.31390757080273957No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC4060.31313629913000557No Hit
CAGGAAACAGCTATGACCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3780.2915406922934535No Hit
CAGGAAANAGCTATGANNGCAGCAAGAGACCAATACCGCATTGTAGTCAA3760.2899981489479854No Hit
CAGGAAANAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT3740.28845560560251743No Hit
CAGGAAANAGCTATGANCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA3730.28768433392978343No Hit
CAGGAAANAGCTATGANNTCCTTCCACCACCAGACAACTGGCCGTCGTTT3710.28614179058431544No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT3600.27765780218424135No Hit
CAGGAAANAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC3560.27457271549330536No Hit
CAGGAAANAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3540.27303017214783737No Hit
CAGGAAACAGCTATGACCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3540.27303017214783737No Hit
CAGGAAANAGCTATGANNTGCCAAAATTAAGGATGCACACCTGTGACACA3540.27303017214783737No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA3530.2722589004751033No Hit
CAGGAAANAGCTATGANCTGACCATCTCTCCTAGACGCCAGTGCAGCTGC3480.26840254211143333No Hit
CAGGAAANAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA3410.26300364040229535No Hit
CAGGAAANAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3320.25606219534768926No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG3220.24834947862034923No Hit
CAGGAAANAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT3200.2468069352748812No Hit
CAGGAAANAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT3190.24603566360214724No Hit
CAGGAAANAGCTATGANNGCAGCTCCCCACAACTTCAACTGGCCGTCGTT3080.2375516752020732No Hit
CAGGAAANAGCTATGANCGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3080.2375516752020732No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT3070.23678040352933918No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG3010.23215277349293517No Hit
CAGGAAACAGCTATGACCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2950.2275251434565311No Hit
CAGGAAANAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2940.22675387178379713No Hit
CAGGAAANAGCTATGANNGTGCAGTCCTCAGGCCACACTAGAAGTAATTA2900.22366878509286112No Hit
CAGGAAANAGCTATGANNGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG2880.22212624174739312No Hit
CAGGAAANAGCTATGANNACACCCGGAGCAGTGCAAATCAACTCCCCCAG2870.2213549700746591No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCCAGACCCACCCGACTGGCC2830.21826988338372308No Hit
CAGGAAANAGCTATGANNAGGCTTTGTGTGTGGGGACTGGCCGTCGTTTT2820.21749861171098905No Hit
CAGGAAANAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG2780.21441352502005306No Hit
CAGGAAANAGCTATGANNGCAGCCACCCTGAACACACTTGGCTATCACCC2750.21209971000185107No Hit
CAGGAAANAGCTATGACCTCCTTCCACCACCAGACAACTGGCCGTCGTTT2720.20978589498364905No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT2710.20901462331091503No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCCAGACCCACCCGACTGGCC2680.20670080829271303No Hit
CAGGAAANAGCTATGANCCATGTGCCCCAGTAGAGAGCAATTAAATTCCT2590.199759363238107No Hit
CAGGAAANAGCTATGANNGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT2550.19667427654717098No Hit
CAGGAAANAGCTATGANCTGACAGCACAAGGAACACTGACCTACAGATAA2520.19436046152896896No Hit
CAGGAAANAGCTATGANNCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT2510.19358918985623497No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA2460.18973283149256492No Hit
CAGGAAANAGCTATGANNCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT2460.18973283149256492No Hit
CAGGAAANAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA2450.18896155981983093No Hit
CAGGAAANAGCTATGANNGGGCAGGCCGAGGACATTAGAAATTTGGGACG2450.18896155981983093No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2450.18896155981983093No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCCAAACCAG2440.18819028814709693No Hit
CAGGAAANAGCTATGANCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2410.18587647312889494No Hit
CAGGAAANAGCTATGACCAGGCTTTGTGTGTGGGGACTGGCCGTCGTTTT2390.18433392978342691No Hit
CAGGAAANAGCTATGANNACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT2370.1827913864379589No Hit
CAGGAAANAGCTATGANCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT2360.1820201147652249No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG2280.1758499413833529No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAAGAAGTGGT2240.17276485469241684No Hit
CAGGAAACAGCTATGACCTGCTCTGGGCTCTGGATACTGGCCGTCGTTTT2230.17199358301968284No Hit
CAGGAAACAGCTATGACCCCTCCGTCCCAGCCTGATTTCTCTCCACCTCC2210.17045103967421485No Hit
CAGGAAACAGCTATGACCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2210.17045103967421485No Hit
CAGGAAACAGCTATGANCCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT2200.16967976800148085No Hit
CAGGAAANAGCTATGANNAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT2190.16890849632874685No Hit
CAGGAAANAGCTATGANCGGCAGACAGCCTCTCCTCCTTTCATCACAAAA2190.16890849632874685No Hit
CAGGAAANAGCTATGANCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA2190.16890849632874685No Hit
CAGGAAANAGCTATGANNTCCTGCCCCACCCTGATACAATATAGTCTCCC2150.1658234096378108No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2130.16428086629234281No Hit
CAGGAAACAGCTATGACCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2110.16273832294687482No Hit
CAGGAAANAGCTATGACCCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT2100.1619670512741408No Hit
CAGGAAACAGCTATGACCCAGGGCTCCAAGAATGTTCCACACATCTTTCT2090.1611957796014068No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2090.1611957796014068No Hit
CAGGAAANAGCTATGACCGCAGCTCCCCACAACTTCAACTGGCCGTCGTT2060.15888196458320478No Hit
CAGGAAANAGCTATGANCGGGCCAGCCAGGAGCAGACACACGGCTCCTCA2020.15579687789226876No Hit
CAGGAAACAGCTATGACCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT2020.15579687789226876No Hit
CAGGAAACAGCTATGANCTGTTTTGCCCCTGAAATGAATATTCATCGTCT2010.15502560621953476No Hit
CAGGAAANAGCTATGANNGGCAGACAGCCTCTCCTCCTTCCACCACCAGA1970.15194051952859877No Hit
CAGGAAANAGCTATGANNGGCAGACCTCGCTCACCTCTTACTGGCCGTCG1950.15039797618313075No Hit
CAGGAAACAGCTATGANCCGACATATCCGAGCCTCACGTTCTAGTTTCTG1950.15039797618313075No Hit
CAGGAAANAGCTATGANNGCATCACTGAGGGAGGATCCTCGGGTCTTACA1940.14962670451039672No Hit
CAGGAAACAGCTATGACCCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1910.14731288949219473No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTCCAGTCGGTACT1900.14654161781946073No Hit
CAGGAAANAGCTATGANCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1900.14654161781946073No Hit
CAGGAAANAGCTATGACCTGCCAAAATTAAGGATGCACACCTGTGACACA1870.14422780280125871No Hit
CAGGAAANAGCTATGANCCACGGCCTCCTTCGGTGAGGACAGGAAGTGGT1870.14422780280125871No Hit
CAGGAAANAGCTATGANCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1830.1411427161103227No Hit
CAGGAAACAGCTATGACCTCCTTCCACCACCAGACAACTGGCCGTCGTTT1830.1411427161103227No Hit
CAGGAAANAGCTATGANCCAGGGCTCCAAGAATGTTCCACACATCTTTCT1780.13728635774665268No Hit
CAGGAAACAGCTATGACCACTCCTCCTGGCCTTCCTCTCCCCACTGGCCG1770.13651508607391868No Hit
NAGGAAANAGCTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1760.1357438144011847No Hit
CAGGAAANAGCTATGANNACAGGGGCCTCTTAAAACTGCATGTGTTCAGC1750.1349725427284507No Hit
CAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATCTAAACCAG1730.13342999938298267No Hit
CAGGAAANAGCTATGACCACTCCTCCTGGCCTTCCTCTTTCAGAGATGGT1720.13265872771024864No Hit
CAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1710.13188745603751464No Hit
CAGGAAACAGCTATGACCCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG1690.13034491269204665No Hit
CAGGAAANAGCTATGACCGTGCAGTCCTCAGGCCACACTAGAAGTAATTA1650.12725982600111063No Hit
CAGGAAANAGCTATGACCCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1650.12725982600111063No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1640.12648855432837663No Hit
CAGGAAANAGCTATGACCGAAAGGCTTCCCCAGGAGCCATGTGTCTAGTG1640.12648855432837663No Hit
CAGGAAANAGCTATGANNCTGGAATTTCAACAGAATGGCATTTATATGAT1620.12494601098290861No Hit
CAGGAAANAGCTATGANNACCTTTACCCAAATAGCTACCACCACGTACCA1610.12417473931017461No Hit
CAGGAAACAGCTATGACCGCAGCTCCCCACAACTTCAACTGGCCGTCGTT1600.1234034676374406No Hit
CAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1590.1226321959647066No Hit
CAGGAAANAGCTATGACCACACCCGGAGCAGTGCAAATCAACTCCCCCAG1590.1226321959647066No Hit
CAGGAAANAGCTATGANNACAGGCAGGAGGGAGTACTACTGGCCGTCGTT1580.12186092429197261No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1570.1210896526192386No Hit
CAGGAAANAGCTATGANCTCCTTCCACCACCAGACAACTGGCCGTCGTTT1570.1210896526192386No Hit
CAGGAAACAGCTATGACCAGGCTTTGTGTGTGGGGACTGGCCGTCGTTTT1560.1203183809465046No Hit
CAGGAAANAGCTATGANNAGCAGCTTTCAAACCTAGACACTCCAACTGGC1540.1187758376010366No Hit
CAGGAAANAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT1540.1187758376010366No Hit
NAGGAAANAGCTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1530.11800456592830258No Hit
CAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1530.11800456592830258No Hit
CAGGAAANAGCTATGACCTCCTGCCCCACCCTGATACAATATAGTCTCCC1500.11569075091010057No Hit
CAGGAAANAGCTATGACCGTGCTCCTTGGGGACGTAGTCGCCTGGGGACT1490.11491947923736656No Hit
CAGGAAACAGCTATGACCCTTCCATGCTGGGCTCTGGACTGGCCGTCGTT1490.11491947923736656No Hit
NAGGAAANAGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1480.11414820756463256No Hit
NAGGAAANAGNTATGANNCTTCCATGTCGCTGCCGGGCCGGGCTGGAGCT1480.11414820756463256No Hit
CAGGAAANAGCTATGACCTGTTTTGCCCCTGAAATGAATATCCAAACCAG1460.11260566421916457No Hit
CAGGAAANAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG1450.11183439254643056No Hit
CAGGAAANAGCTATGACCCCCCAGGCCATTGTTGGCCTCACAGGAGTAGT1450.11183439254643056No Hit
NAGGAAANAGCTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1440.11106312087369656No Hit
CAGGAAANAGCTATGANCGCAGCAAGAGACCAATACCGCATTGTAGTCAA1440.11106312087369656No Hit
CAGGAAANAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAATAGTCT1410.10874930585549453No Hit
CAGGAAANAGCTATGACCGGCAGACCTCGCTCACCTCTTACTGGCCGTCG1390.10720676251002653No Hit
NAGGAAANAGNTATGANNTGTTTTGCCCCTGAAATGAATATTCATCGTCT1390.10720676251002653No Hit
CAGGAAANAGCTATGACCGCAGCCACCCTGAACACACTTGGCTATCACCC1360.10489294749182453No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1350.10412167581909051No Hit
CAGGAAACAGCTATGANCACCAAGACCCCAGCAGCATTAAAAAGGCTGCT1330.10257913247362252No Hit
CAGGAAANAGCTATGANCAGGCTTTGTGTGTGGGGACTGGCCGTCGTTTT1330.10257913247362252No Hit
CAGGAAANAGCTATGACCACAGGCAGGAGGGAGTACTACTGGCCGTCGTT1320.1018078608008885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)