FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81301
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT5140.6322185458973445No Hit
NNGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT3500.4304990098522773No Hit
NNGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT3430.4218890296552318No Hit
NNGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA3400.4181990381422123No Hit
NNGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG3310.4071290636031537No Hit
NNGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA3030.3726891428149715No Hit
NNGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2730.3357892276847763No Hit
NNGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA2720.33455923051376985No Hit
NNGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2510.30872928992263315No Hit
NNGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA2510.30872928992263315No Hit
NNGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG2480.30503929840961364No Hit
NNGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA2430.2988893125545811No Hit
NNGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2340.28781933801552256No Hit
NNGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT2320.2853593436735095No Hit
NNGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG2270.27920935781847706No Hit
NNGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2260.27797936064747053No Hit
NAGGANNNNGNTATGNNNGCATCAGATTCACTTCCACTGTCGTCAGAACT2240.27551936630545754No Hit
NNGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCGGGGATCCTCG2110.25952940308237293No Hit
NNGGANNNNGNTATGNNNCAGGGCTCCAAGAATGTTCCACACATCTTTCT2100.2582994059113664No Hit
NNGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA2060.25337941722734036No Hit
NNGGANNNNGNTATGNNNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT2010.24722943137230785No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC2010.24722943137230785No Hit
NNGGANNNNGNTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1920.2361594568332493No Hit
NNGGANNNNGNTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1920.2361594568332493No Hit
NNGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1880.23123946814922325No Hit
NNGGANNNNGNTATGNNNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1860.22877947380721023No Hit
NNGGANNNNGNTATGNNNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1830.2250894822941907No Hit
NAGGANNNNGNTATGNNNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1780.21893949643915822No Hit
NNGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1770.2177094992681517No Hit
NNGGANNNNGNTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1690.20786952190009964No Hit
NNGGANNNNGNTATGNNNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1610.19802954453204757No Hit
NNGGANNNNGNTATGNNNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1570.19310955584802156No Hit
NNGGANNNNGNTATGANNTAGAGGACACCCAAGGCCACCGGGGATCCTCG1570.19310955584802156No Hit
NNGGANNNNGNTATGANNAAGGACAAGGTGAGGCAGCACCCCACCCTGCT1550.19064956150600854No Hit
NAGGANNNNGNTATGNNNCATGGTTGGGGAAAAACTCCTCATCTTCATGT1540.18941956433500204No Hit
NNGGANNNNGNTATGNNNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1520.18695956999298902No Hit
NNGGANNNNGNTATGNNNGGGGAGACTGGGCCATACGATAGGCTTAATCC1510.18572957282198252No Hit
NNGGANNNNGNTATGNNNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA1490.1832695784799695No Hit
NNGGANNNNGNTATGNNNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1480.182039581308963No Hit
NAGGANNNNGNTATGANNGCATCAGATTCACTTCCACTGTCGTCAGAACT1480.182039581308963No Hit
NNGGANNNNGNTATGNNNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA1470.18080958413795647No Hit
NNGGANNNNGNTATGANNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA1470.18080958413795647No Hit
NNGGANNNNGNTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1460.17957958696694998No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1450.17834958979594345No Hit
NNGGANNNNGNTATGNNNTCCCACCACTGACTCCAGGGGAAGGAGGAGGA1450.17834958979594345No Hit
NNGGANNNNGNTATGANNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC1420.17465959828292396No Hit
NNGGANNNNGNTATGANNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1410.17342960111191744No Hit
NAGGANNNNGNTATGNNNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1380.16973960959889792No Hit
NAGGANNNNGNTATGNNNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG1370.16850961242789142No Hit
NNGGANNNNGNTATGANNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT1360.16727961525688492No Hit
NNGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC1360.16727961525688492No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA1360.16727961525688492No Hit
NNGGANNNNGNTATGNNNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA1330.1635896237438654No Hit
NAGGANNNNGNTATGNNNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA1280.15743963788883286No Hit
NNGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCGTCTC1280.15743963788883286No Hit
NNGGANNNNGNTATGNNNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1260.15497964354681984No Hit
NNGGANNNNGNTATGANNTGGTTTCCGTTTGTCTCCTCTCTCATACCTTT1250.15374964637581334No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCTGGGTGCTGGTG1230.15128965203380032No Hit
NNGGANNNNGNTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT1200.1475996605207808No Hit
NNGGANNNNGNTATGNNNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG1180.14513966617876778No Hit
NNGGANNNNGNTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1170.14390966900776128No Hit
NNGGANNNNGNTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG1140.14021967749474176No Hit
NAGGANNNNGNTATGNNNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1130.13898968032373527No Hit
NNGGANNNNGNTATGANNCAGCTGTGTCTCCAGCTCCAGCACCTGCACCA1120.13775968315272877No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA1100.13529968881071575No Hit
NAGGANNNNGNTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA1100.13529968881071575No Hit
NAGGANNNNGNTATGANNCGACATATCCGAGCCTCACGTTCTAGTTTCTG1090.13406969163970922No Hit
NNGGANNNNGNTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC1090.13406969163970922No Hit
NNGGANNNNGNTATGNNNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT1090.13406969163970922No Hit
NNGGANNNNGNTATGNNNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT1080.13283969446870272No Hit
NAGGANNNNGNTATGNNNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC1060.13037970012668973No Hit
NAGGANNNNGNTATGNNNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA1050.1291497029556832No Hit
NNGGANNNNGNTATGNNNAGGACTTGCCCACTCTCCCAGGAAGCAGAAGT1040.1279197057846767No Hit
NNGGANNNNGNTATGANNCCCGTGACAGCTCCTGCAATTACAAAATGGCT1040.1279197057846767No Hit
NNGGANNNNGNTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG1030.12668970861367018No Hit
NNGGANNNNGNTATGNNNGCAAAACACCTTAACACTCGGCTTCTATTTGC1020.12545971144266369No Hit
NNGGANNNNGNTATGNNNAAAGGCAGCAGGGTCGATGGCTTGCAGGTCTT1000.12299971710065068No Hit
NAGGANNNNGNTATGNNNTGGGGATGAGGACAGCAGGGCAGTCACCATCT970.11930972558763114No Hit
NAGGANNNNGNTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA970.11930972558763114No Hit
NNGGANNNNGNTATGNNNTGAGTTAACGTGATTTAGGACCCTGGCTGTTT950.11684973124561812No Hit
NNGGANNNNGNTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG950.11684973124561812No Hit
NAGGANNNNGNTATGANNTTGGCTGGGGCGACTGACGCAGAAGAGACCCA930.11438973690360511No Hit
NNGGANNNNGNTATGANNCTTGGTGGAGCCATAGGCCGCGTGCACGCGCA930.11438973690360511No Hit
NNGGANNNNGNTATGNNNCTCAGCTCTGCAGCCTCCGCTGCCATGGCTGT910.1119297425615921No Hit
NAGGANNNNGNTATGNNNGCTGCTGACCCAACCCTACCTCTGCAGCTAGC910.1119297425615921No Hit
NNGGANNNNGNTATGANNAGAGGCACAGGTGGAGAGGGACGAGACCCTGA890.10946974821957911No Hit
NNGGANNNNGNTATGNNNATCCACTGTTGGCCAACACACCTGGCCTTCAA890.10946974821957911No Hit
NAGGANNNNGNTATGNNNGAGTACTCCTCGTCCAGGCGCCTGTTCATCTC890.10946974821957911No Hit
NAGGANNNNGNTATGNNNTGCTGGGAGAGGGGGATGAGTAGACAGGGCCA880.10823975104857259No Hit
NNGGANNNNGNTATGNNNCTGGTCCTCATCCTTGGGGCCTGAGAGCCTGT880.10823975104857259No Hit
NAGGANNNNGNTATGNNNTAGAGGACACCCAAGGCCACCGGGGATCCTCG880.10823975104857259No Hit
NNGGANNNNGNTATGNNNAGAGGCACAGGTGGAGAGGGCAGGAAACAGCT880.10823975104857259No Hit
NAGGANNNNGNTATGANNAGGACCACCCCTCCATCCTGCTGCCTGGCTTG870.10700975387756609No Hit
NAGGANNNNGNTATGANNCATGGTTGGGGAAAAACTCCTCATCTTCATGT860.10577975670655958No Hit
NNGGANNNNGNTATGANNACAGGAGACAGGATTGGGGCAGCCCCGGCCCT850.10454975953555308No Hit
NNGGANNNNGNTATGANNCTCAGCTCTGCAGCCTCCGCACAGACCCCACA840.10331976236454656No Hit
NNGGANNNNGNTATGNNNCTAGGGTGGGGTGAGGGGAGTCAGCAGGTGCT840.10331976236454656No Hit
NAGGANNNNGNTATGNNNTGCAATCTTGAGCTCCTGGGCATGTCTCTGTT830.10208976519354006No Hit
NNGGANNNNGNTATGNNNGGCGGGCCCTGATATAGAGCCACTGGCCGTCG830.10208976519354006No Hit
NNGGANNNNGNTATGNNNCCCTGCGGCTCCTTTCAAGTGCAGAGGAAGCC820.10085976802253355No Hit
NNGGANNNNGNTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA820.10085976802253355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)