FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003245710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192662
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAANAGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT32131.667687452637261No Hit
CAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC31921.6567875346461678No Hit
CAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT30111.5628406224372215No Hit
CAGGAAANAGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC29181.5145695570480946No Hit
CAGGAAANAGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT26651.3832514974411145No Hit
CAGGAAANAGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG24071.2493382192648264No Hit
CAGGAAANAGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG22891.1880910610291597No Hit
CAGGAAANAGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC21581.1200963345132926No Hit
CAGGAAANAGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC21071.0936251051063521No Hit
CAGGAAANAGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT18990.9856640126231432No Hit
CAGGAAANAGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT18250.947254777797386No Hit
CAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC12700.6591855166042084No Hit
CAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT12620.6550331668933157No Hit
CAGGAAANAGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG12070.6264857626309288No Hit
CAGGAAANAGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC12000.6228524566338978No Hit
CAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC11750.6098763637873581No Hit
CAGGAAANAGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG11440.5937860086576491No Hit
CAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT11370.5901527026606181No Hit
CAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT10110.5247531947140588No Hit
CAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG9930.5154104078645504No Hit
CAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG9820.5097009270120729No Hit
CAGGAAANAGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT9610.49880100902097974No Hit
CAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC9530.4946486593100871No Hit
CAGGAAANAGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA8760.45468229334274535No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA8040.4173111459447114No Hit
CAGGAAANAGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA7540.39135896025163236No Hit
CAGGAAANAGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT7460.38720661054073974No Hit
CAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC7250.37630669254964655No Hit
CAGGAAANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT7040.36540677455855336No Hit
CAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT6980.36229251227538384No Hit
CAGGAAANAGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC6410.3327070205852737No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG6290.3264784960189347No Hit
CAGGAAANAGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA5870.30467866003674826No Hit
CAGGAAANAGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC5170.2683456000664376No Hit
CAGGAAANAGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG5100.2647122940694065No Hit
CAGGAAANAGCTATGANNAATGCAAGGGACCACCAGGCTGAGGCAGGAGA4810.24966002636742066No Hit
CAGGAAANNGCTATGANNGGGGAGACTGGGCCATACGATAGGCTTAATCC4720.24498863294266643No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT4650.24135532694563536No Hit
CAGGAAANNGCTATGANNGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG4520.23460775866543482No Hit
CAGGAAANAGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTCT4290.22266975324661842No Hit
CAGGAAANNGCTATGANNCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG4170.21644122868027948No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC4100.21280792268324839No Hit
CAGGAAANAGCTATGANNTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC3940.20450322326146306No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCGCTTGATCCCG3870.20086991726443199No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCATA3850.19983182983670886No Hit
CAGGAAANAGCTATGANNCGGAGTCACACTCTGTCACCCACGCCCGGCCT3750.19464139269809302No Hit
CAGGAAANNGCTATGANNCGAGGGGCAGAGATGGCGTAGGTGCCAGTTCT3580.18581764956244615No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACACACGGCTCCTCA3500.1816652998515535No Hit
CAGGAAANNGCTATGANNCGGGACTTCCCGAGCACAGGGCTCGGGCCAGA3330.17284155671590662No Hit
CAGGAAANAGCTATGANNTGCTGCCGAGAGAGGACAGTGATCCCCCAAAA3220.1671320758634292No Hit
NAGGAAANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3210.16661303214956763No Hit
CAGGAAANAGCTATGANNGAGGCGGAAGTGGTGCTCATCCACGTACTGTG3210.16661303214956763No Hit
CAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3180.1650559010079829No Hit
CAGGAAANAGCTATGANNTGCCAGTCTTCCCCTTCCCTGTGAGAGAAGCA3170.16453685729412132No Hit
NAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT3160.1640178135802597No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC3150.16349876986639816No Hit
CAGGAAANNGCTATGANNCCGTAGCCACACCCCTTAAGCCAGAGCCGACT3140.16297972615253656No Hit
CAGGAAANNGCTATGANNGCCCAGGCCCAGTGACTCATGAGTAACCTTGA3120.1619416387248134No Hit
CAGGANANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT3100.16090355129709025No Hit
NAGGAAANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC3070.15934642015550549No Hit
NAGGANANNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC3070.15934642015550549No Hit
CAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2970.15415598301688968No Hit
NAGGANANNGCTATGANNTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC2920.15156076444758176No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACAGCT2890.15000363330599703No Hit
NAGGAAANNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2850.1479274584505507No Hit
CAGGAAANAGCTATGANNTGAAGTGGCTCCATCCTTCCATTTGTTTATAT2840.14740841473668914No Hit
CAGGAAANNGCTATGANNGGGCCAGCCAGGAGCAGACGCACGGCTCCTCA2800.1453322398812428No Hit
CAGGAAANNGCTATGANNATCGCACCACTGCACTCCAGCACTGGCCGTCG2790.14481319616738123No Hit
CAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2780.14429415245351965No Hit
NAGGAAANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2750.1427370213119349No Hit
NAGGANANNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT2720.14117989017035015No Hit
CAGGAAANAGCTATGANNCCCCAGGTGGCCTTGCCAAAATTATCTGCGTA2720.14117989017035015No Hit
NAGGAAANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2710.14066084645648858No Hit
CAGGANANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2680.13910371531490384No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC2600.13495136560401116No Hit
CAGGAAANAGCTATGANNAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC2590.1344323218901496No Hit
CAGGAAANAGCTATGANNTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG2580.133913278176288No Hit
CAGGAAANAGCTATGANNGCTTCTATATGAGCGCATCTCACCTGAATTTG2510.13027997217925694No Hit
CAGGAAANAGCTATGANNAACCAACACTGCTGCTAATTGTGTTTGTGTCC2490.12924188475153378No Hit
NAGGAAANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2470.12820379732381063No Hit
CAGGAAANAGCTATGANNCACCGCTGTCCCTTCCCAAGGGGCTGAAGGTG2420.1256085787545027No Hit
NAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2410.12508953504064113No Hit
NAGGANANNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT2400.12457049132677954No Hit
CAGGAAANAGCTATGANNGTTTTCACTGGCTCCCGGGGGAGCTGTCACTA2340.12145622904361005No Hit
CAGGAAANNGCTATGANNGCAAAACACCTTAACACTCGGCTTCTATTTGC2330.12093718532974847No Hit
CAGGAAANAGCTATGANNCCTGGACGCAATCACCCAGGCCAGCTAAGTGA2260.11730387933271741No Hit
CAGGANANNGCTATGANNCGTCAGTGGACCAAGCACGAGAGGAAGCGCCG2250.11678483561885582No Hit
NAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2220.11522770447727107No Hit
CAGGAAACNGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2220.11522770447727107No Hit
CAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2170.11263248590796315No Hit
NAGGAAANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2150.11159439848024001No Hit
CAGGAAANNGCTATGANNCGGAGTCACACTCTGTCACCCAGGCTGGAGTG2150.11159439848024001No Hit
CAGGAAANAGCTATGANNGGAGGCTGAGGTGGGAGGATCTCTTGAGCCCA2130.11055631105251684No Hit
CAGGAAACNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2130.11055631105251684No Hit
NAGGAAANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2120.11003726733865525No Hit
CAGGANANNGCTATGANNCCAGGTAGCCAGACTCCCACTCAGGAAGTCTC2110.10951822362479369No Hit
CAGGAAANNGCTATGANNCTGGCCTCCTTCAATGCATTTAATGGCCTTAT2090.10848013619707052No Hit
CAGGAAACAGCTATGANCCCGTAGCCACGCCCCTATTGCAGGAAACAGCT2080.10796109248320893No Hit
NAGGAAANAGCTATGANNACAGTGTCCCCATCAGGCTCCGGGACAGCATC2080.10796109248320893No Hit
NAGGANANNGCTATGANNGGTTCTCGCGCTGGGTCTCGTGCAGCCGGGCG2080.10796109248320893No Hit
CAGGAAACAGCTATGANCACAGTGTCCCCATCAGGCTCCGGGACAGCATC2080.10796109248320893No Hit
CAGGAAANNGCTATGANNTCCTCTTCATTTTCACGGTGCCATTCTGATTC2010.10432778648617785No Hit
CAGGAAANAGCTATGANNCTTGAGCTCCTCGGACACGGCAGGAAACACCT2010.10432778648617785No Hit
NAGGAAANAGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1970.10225161163073153No Hit
CAGGAANNNGCTATGANNCCGTAGCCACGCCCCTATTGCAGGAAACAGCT1960.10173256791686995No Hit
CAGGAANNNGCTATGANNTGGGGATGAGGACAGCAGGGCAGTCACCATCT1960.10173256791686995No Hit
NAGGAAANNGCTATGANNCAGGGCTCCAAGAATGTTCCACACATCTTTCT1960.10173256791686995No Hit
NAGGANANNGCTATGANNACGTTCTGGGCTGTGGTGTGCCAGGCCACAAT1960.10173256791686995No Hit
CAGGANANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1950.10121352420300839No Hit
CAGGAANNNGCTATGANNGGGACCAGTCCAATAACCCCTGAGGGGTATAT1940.1006944804891468No Hit
NAGGAAANNGCTATGANNTTTTTGTGCCCTTCTGCCCGCAGAACTCGCCC1930.10017543677528522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)