FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003245726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003245726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130060
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAACAGCTATGACCTCCTCGCTTCGCGTTCTTCCACTAAAGAACCT50503.882823312317392No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCACTGGCCGTCGT42893.297708749807781No Hit
CAGGAAACAGCTATGACCTGGGATTCCTCTTCTTAGCTTCTTTCATCAAC41653.2023681377825617No Hit
CAGGAAACAGCTATGACCCGACATATCCGAGCCTCACGTTCTAGTTTCTG41203.167768722128249No Hit
CAGGAAACAGCTATGACCTTGGCTGGGGCGACTGACGCAGAAGAGACCCA40603.1216361679224973No Hit
CAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA38392.951714593264647No Hit
CAGGAAACAGCTATGACCGGGACCAGTCCAATAACCCCTGAGGGGTATAT33682.5895740427495No Hit
CAGGAAACAGCTATGACCTGAGGGGCTGCTATCCCTTTATGAGAGGGAGA26962.072889435645087No Hit
CAGGAAACAGCTATGACCCCTCTGTTCACCAGTGCCGTCTCTTCTTTTAG24781.9052744886975244No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCTGAGGCAGGAGA20591.5831154851606952No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGGGCACGGGAACA20051.541596186375519No Hit
CAGGAAACAGCTATGACCGCAGCTGGGAGATGCCTGGAAGGCAGAGATAG19101.4685529755497462No Hit
CAGGAAACAGCTATGACCGCAAAACACCTTAACACTCGGCTTCTATTTGC18511.4231892972474243No Hit
CAGGAAACAGCTATGACCGCCCAGCAATGGAGAGGCTACCAGCTGATCCA17451.3416884514839305No Hit
CAGGAAACAGCTATGACCCGGTGAGGTGGAGGGAGTCAGGACTGGCCGTC15161.1656158695986467No Hit
CAGGAAACAGCTATGACCAGAGACCAGATGCATAAGCACAGCAGCATTCC14861.1425495924957711No Hit
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTAGA13000.9995386744579424No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTTGGC12940.9949254190373674No Hit
CAGGAAACAGCTATGACCCATCAGGCCCCACCTCCAACCAGGAAACAGCT12300.9457173612178994No Hit
CAGGAAACAGCTATGACCTGACAGCACAAGGAACACTGACCTACAGATAA11710.9003536829155775No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGTCTGCAGCCTGG11660.8965093033984316No Hit
CAGGAAACAGCTATGACCGGCTGGCACAAAGAACAGCACTGACTGTTTAT10860.8349992311240966No Hit
CAGGAAACAGCTATGACCAAGTTGGTCAGCCCTGGTGACTCATTGGATAT10730.8250038443795172No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAGGGACGCAG10680.8211594648623712No Hit
CAGGAAACAGCTATGACCTGAAGTGGCTCCATCCTTCCATTTGTTTATAT9950.7650315239120407No Hit
CAGGAAACAGCTATGACCTGGGAAGAGCTCCCGGATCAGGAAGGCTCGGC9590.7373519913885899No Hit
CAGGAAACAGCTATGACCTGCCCAGTTGTTTCCAGAACCTTGTTTTTGAC9580.7365831154851608No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGATGTTGACAT9170.7050592034445641No Hit
CAGGAAACAGCTATGACCAGCTGGACGGGGTCCTTCTTCCCTCTCTCCTT9110.7004459480239889No Hit
CAGGAAACAGCTATGACCGCTGCTGACCCAACCCTACCCTGTCCAGCCCC8870.6819929263416884No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCCTGCCCAGGCC8680.667384284176534No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGCTGCCTGGCTTG8640.6643087805628172No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACAGTAGTCT8490.6527756420113794No Hit
CAGGAAACAGCTATGACCGCTTCTCGGCCATGTGGGTTAAACTCGGGGAT7510.577425803475319No Hit
CAGGAAACAGCTATGACCCCTGGACGCAATCACCCAGGCCAGCTAAGTGA6710.5159157312009842No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACACACTGGGGCC6630.5097647239735507No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGGCTCTTTT6600.5074580962632631No Hit
CAGGAAACAGCTATGACCGCTACGGGCAATCACTCTCCTACTCCCAACCA6420.49361833000153776No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCAGGCTGCTGACC6260.48131631554667076No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAACAGGGAC5760.44287252037521146No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGC5520.42441949869291096No Hit
CAGGAAACAGCTATGACCTCCCACTTGCTCTTTGAAGGCAATTTCATTCT5430.4174996155620483No Hit
CAGGAAACAGCTATGACCGGGCAGGCCGAGGACATTAGAAATTTGGGACG5210.40058434568660617No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAGCAGTAATGGA4920.37828694448715977No Hit
CAGGAAACAGCTATGACCGCAGCAAGAGACCAATACCGCATTGTAGTCAA4770.366753805935722No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAATGAAACTTGGTT4730.36367830232200526No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCCATGCCTGCCGT4390.3375365216054129No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATCAC4300.3306166384745502No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGC4090.3144702445025373No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCACGGTGGCTCAT4060.31216361679224974No Hit
CAGGAAACAGCTATGACCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGC3910.3006304782408119No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACACACGGC3870.2975549746270952No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAAAGGCTCCGTGA3760.2890973396893741No Hit
CAGGAAACAGCTATGACCAAGGACAAGGTGAGGCAGCAAGTGCTGATGTA3700.284484084268799No Hit
CAGGAAACAGCTATGACCTGCTTTGGGGCCATGGAGTTCACTTGGTAAGG3600.2767953252345072No Hit
CAGGAAACAGCTATGACCCGCTTCTGGGGACTTTGCTCTTGGATTCTTTT3390.2606489312624942No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGTGAGGACAGAT3300.25372904813163155No Hit
CAGGAAACAGCTATGACCTTCCTGATGGGATTCAGCCATCAACAGTAGGA3280.25219129632477316No Hit
CAGGAAACAGCTATGACCTGGGTTGCCTGGGACTGAGAGGGTCCTGGGAC3220.24757804090419808No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGACTGAGGGGCAG3220.24757804090419808No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCAACAGGGACGCA3140.24142703367676455No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCTCCCAACTCTT3070.23604490235276027No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACACTCCCCGGTGAC2970.22835614331846837No Hit
CAGGAAACAGCTATGACCCCGTAGCCACGCCCCTATTGTCTGACCACGCT2960.2275872674150392No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCCTCTCCCAACT2900.2229740119944641No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAGAAGTCTTTTAA2780.21374750115331384No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGGGGGCCTGGCT2750.2114408734430263No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCAGCATGATCCT2680.20605874211902198No Hit
CAGGAAACAGCTATGACCCGTTAGCTGCCTCTCCTGCCCTGGGACAACGG2650.20375211440873442No Hit
CAGGAAACAGCTATGACCAGGACCACCCCTCCATCCTGGCCTGGCTTGTC2470.18991234814700908No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTCTAGG2460.18914347224357989No Hit
CAGGAAACAGCTATGACCTCTCTTGGGGGCACTCTCCCCTGCCCCCTGCA2350.18068583730585883No Hit
CAGGAAACAGCTATGACCGGAAGGGGAATGGGAAGGAGCAGACGCACGGC2250.172997078271567No Hit
CAGGAAACAGCTATGACCTCCAGTGGTGGGGCTCAACAGTGGGTGGAGGT2200.16915269875442104No Hit
CAGGAAACAGCTATGACCATTAGCCAGGTGTGGTGGCGTGCGCCTGTTTT2040.15685068429955404No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCACAACAGGGACG1990.15300630478240812No Hit
CAGGAAACAGCTATGACCGCCCTGGCCACTGTGGTTAAACCCCGTCTCTA1790.1376287867138244No Hit
CAGGAAACAGCTATGACCTCCCTTAAACTTGGGATTGCTCCACTGCAGTC1680.12917115177610333No Hit
CAGGAAACAGCTATGACCGGTAGCAACGTCCCACCCAGCTGAATAGTTGT1620.12455789635552822No Hit
CAGGAAACAGCTATGACCACCCAGTTAGAGCCATGGTTCCACTAGACTCT1540.11840688912809472No Hit
CAGGAAACAGCTATGACCAGAAAGAAGAAGCAGAGCAAAAACGTATGTAT1530.11763801322466554No Hit
CAGGAAACAGCTATGACCAGTGGGAGGAGCTCTCGGTCCCCTCTTTTAGG1520.11686913732123635No Hit
CAGGAAACAGCTATGACCAATGCAAGGGACCACCAGGCCTTCCGTCGCAA1520.11686913732123635No Hit
CAGGAAACAGCTATGACCTTCCCTGTGCCTGGCCCTATAGTGAGTCGTAT1510.11610026141780716Clontech Universal Primer Mix Long (95% over 22bp)
CAGGAAACAGCTATGACCAGACAGGGTCTTGCTATGTTGCCCAGGCTGGT1400.1076426264800861No Hit
AAGGAAACAGCTATGACCAGGCACCAACAGTACAAAGCAGATACAAAGCA1400.1076426264800861No Hit
CAGGAAACAGCTATGACCTGTTGTCTTTCAGGGGCCGCGCCATTGAACTG1320.10149161925265263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)